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pGL4.26-Ccnd2PromoterRegion2(0.5kb)

Plasmid

#228059

Purpose

0.5 kb from mouse Ccnd2 promoter in front of firefly luciferase reporter

Depositor

Kenneth Chen

Article

Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

Insert

Ccnd2 promoter (Ccnd2 Mouse)

Use

Luciferase

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.26-Igf2Promoter

Plasmid

#228060

Purpose

0.9 kb from mouse Igf2 promoter in front of firefly luciferase reporter

Depositor

Kenneth Chen

Article

Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

Insert

Igf2 promoter (Igf2 Mouse)

Use

Luciferase

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-His:CRY2:nls:VPR

Plasmid

#135989

Purpose

Expresses the CRY2 blue light-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Inserts

CRY2 domain fused to the VPR activation domain
CRY2 domain fused to the VPR activation domain

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-FNTA-FLAG

Plasmid

#86858

Purpose

To express FNTA with a FLAG tag at C terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

FNTA (FNTA Human)

Tags

FLAG

Expression

Mammalian

Mutation

The sequence of the insert is isoform 2, which di…

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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eDrcKIT

Plasmid

#184001

Purpose

Optically controlled eDrcKIT expression in mammalian cells

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrcKIT (KIT Human)

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

Plasmid

#124660

Purpose

reporter protein which emits bright blue fluorescence

Depositor

Yutaka Kuroda

Article

Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

Insert

Gaussia luciferase-C9D-TGinsert

Tags

IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

Expression

Bacterial

Mutation

changed glutamic acid 100 to alanine, and glycine…

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR4 PARP2 E614Q

Plasmid

#192545

Purpose

pENTR plasmid Arabidopsis thaliana PARP2 CDS, E614Q mutation, no stop codon

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

poly(ADP-ribose) polymerase 2 E614Q (AT3G18250 Mustard Weed)

Use

Gateway entry vector

Mutation

changed Glu 614 to Gln

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pPICZ-aC-KHC-BZ

Plasmid

#160427

Purpose

Expression of c-luciferase from sp. Kornikeria hastingsi carriebowae (Belize)

Depositor

Todd Oakley

Article

Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

Insert

luciferase

Use

Luciferase

Tags

6x Histidine tag, Yeast alpha secretion factor, c…

Expression

Yeast

Promoter

AOX

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGBT9-Hook2

Plasmid

#198524

Purpose

Expression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

Insert

Hook2 (HOOK2 Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

His488Gln substitution

Promoter

ADH1

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pZNpF-∆48∆11

Plasmid

#245369

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆48∆11 (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

11-bp deletion, H96Wfs*4, plus synthetic 48-bp up…

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO-puro SNAP-CD86-eGFP-FKBP(f36v)

Plasmid

#200283

Purpose

Membrane protein fusion with extracellular SNAPtag and intracellular FKBP(f36v) dimerization domain and GFP reporter under Tetracycline-inducible promoter

Depositor

Kristina Ganzinger

Article

Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

Insert

SNAPtag-eGFP-CD86tm-eGFP-FKBP(f36v)

Use

Lentiviral

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-ε ear

Plasmid

#196670

Purpose

Expresses GST-AP-4 ε ear (residues 840-1137) in bacteria

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

Insert

AP-4 ε ear (840-1137) (AP4E1 Human)

Tags

GST

Expression

Bacterial

Mutation

silent mutations in codons 905, 1129 and 1137

Promoter

tac

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1-TSF-ε

Plasmid

#196583

Purpose

Expresses the two Strep/one FLAG (TSF)-tagged human AP-4 ε subunit in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

Insert

AP-4 ε subunit (AP4E1 Human)

Tags

two Strep/one FLAG (TSF)

Expression

Mammalian

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pEE071

Plasmid

#176828

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

710

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE073

Plasmid

#176830

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

712

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE074

Plasmid

#176831

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

713

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE055

Plasmid

#176812

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

606

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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eDrMet

Plasmid

#183999

Purpose

Optically controlled eDrcMet expression in mammalian cells

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrMet (MET Human)

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pKLD-CAG-DYSF-CPL

Plasmid

#131458

Purpose

Encodes Dysferlin protein (Therapeutic gene) protein to cure LGMD2B along with luciferase enzyme for in vivo live imaging

Depositor

Michele Calos

Article

Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

Insert

DYSF (DYSF Human)

Expression

Bacterial and Mammalian

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pPICZ-aC-VTS-US

Plasmid

#160425

Purpose

Expression of c-luciferase from sp. Vargula tsujii (USA)

Depositor

Todd Oakley

Article

Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

Insert

luciferase

Use

Luciferase

Tags

6x Histidine tag, Yeast alpha secretion factor, c…

Expression

Yeast

Mutation

See depositor comments below

Promoter

AOX

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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