We narrowed to 3,245 results for: Streptococcus
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Plasmid#183679PurposeExpresses eNme2-C-BE4 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertrAPOBEC1-neNme2-C-2xUGI
UseCRISPRExpressionMammalianMutationNme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET42b-AncBE4max
Plasmid#165157PurposeExpresses NLS-AncBE4max-NLS with an N-terminal His Tag (6xHis) for bacterial expressionDepositorInsertAncBE4max
Tags6xHisExpressionBacterialAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SCP1-dSa-VPR mini.
Plasmid#99685PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoterDepositorPublicationInsertdCas9
UseAAVTagsVPR miniMutationdead Cas9Available sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40_scFv_GCN4_sfGFP-VPR-GB1_NLS
Plasmid#79373PurposeRecruits VPR to a compatible Cas9 protein for transcriptional activationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGCN4-sfGFP-VPR
UseCRISPRExpressionMammalianAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAG-Cas9
Plasmid#89995PurposeExpresses high levels of WT Cas9 for genome editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthCas9
Tags3xFlag, SV40 NLS, and T2A peptideExpressionMammalianPromoterCAGAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLEX_307_ms2_VPR
Plasmid#79376PurposeRecruits VPR to compatible gRNAs for transcriptional activationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertms2-VPR
UseCRISPRExpressionMammalianAvailable sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gRNA-his-HYB
Plasmid#64333Purposehis3 deletion gRNA cassette carried by pRS42HDepositorInsertgBlock product of his3 deletion gRNA cassette
ExpressionYeastAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gRNA-leu-HYB
Plasmid#64332Purposeleu2 deletion gRNA cassette carried by pRS42HDepositorInsertgBlock product of leu2 deletion gRNA cassette
ExpressionYeastAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti_dCas9-KRAB-MeCP2_Blast
Plasmid#221501PurposeLentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vectorDepositorInsertdCas9-KRAB-MeCP2-T2A-Blast
UseCRISPR and LentiviralExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AD59_pEP.DonorCLYBL.TS
Plasmid#199227PurposeDonor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertExpression unit for PuroR.T2A.EGFP selectable and reporter markers
UseGene targeting donor plasmidExpressionMammalianPromoterHuman PGK1 gene promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AY27_pU6.gRNA.CLYBL
Plasmid#199238PurposeExpression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locusDepositorInsertS. pyogenes gRNA spacer
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterRNA polymerase III promoter for human U6 snRNAAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
nontarget S. pyogenes scaffold CARGO array
Plasmid#191319PurposeContains an array of nontargeting guide RNAsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertArray of guide RNAs
UseCRISPRTagsmCherryExpressionMammalianAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBHM2404
Plasmid#173441PurposepRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR templateDepositorInsertmNeonGreen-CBP-2XFLAG-NAT1
TagsCBP-2xFLAGExpressionBacterial and YeastAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMDlg/pRRE-int-MS2M
Plasmid#166032PurposeExpression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at C terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.DepositorInsertGag-Pol-MS2
UseLentiviralMutationD64V mutation within the integrase within PolAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
miniCGBE1-NG (pBM1182)
Plasmid#140257PurposeCMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBE4max(R33A)_NG-ΔUGI
ExpressionMammalianMutationR33A in rAPOBEC1, NG mutations in SpCas9, D10A in…PromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-saCBE N-terminal
Plasmid#137182PurposeAAV genome: expresses the N-terminal of S. aureus v5 AAV-CBE from the Cbh promoter, U6-sgRNADepositorInsertv5 AAV-saCBE N-terminal
UseAAVMutationCas9 D10APromoterCbhAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PX458-3xHA-FnCas9
Plasmid#130969Purpose3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFnCas9
UseCRISPRTags3xHA-NLS and NLS-T2A-EGFPExpressionMammalianPromoterCbhAvailable sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pACT1:Cas9-GFP, U6:sgTK
Plasmid#122852PurposeExpresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK geneDepositorInsertsCas9-GFP
U6-sgTK
UseCRISPR; Cas9-gfp plasmid for genome editing of cr…TagseGFPPromoterCryptosporidium parvum U6 and Cryptosporidium par…Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BB3cH_pGAP_23*_pPFK300_Cas9
Plasmid#104912PurposehCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on HygDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRExpressionYeastAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ciCas9_pcDNA5
Plasmid#100550PurposeExpresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertciCas9
UseCRISPR; Frt, flp-inTagsFLAGExpressionMammalianAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only