Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 55,028 results for: ran

Showing: 1921 - 1940 of 55028 results

  7. pSL0747 (pDonor_L-MmeI)

    Plasmid
    #130647
    Purpose
    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchCAST donor DNA (L*)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Mutation
    TGTTGATG --> TGTTGGAG
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130647-plasmid-map-sequence-id-255647

    Enlarge

  8. pSL0746 (pDonor_R-MmeI)

    Plasmid
    #130646
    Purpose
    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchCAST donor DNA (R*)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Mutation
    TGTTGATA --> TGTTGGAA
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130646-plasmid-map-sequence-id-255642

    Enlarge

  9. pFS_1061_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_GGC(BbsI)-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid
    #184738
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 and transcriptionally insulated FsCRISPR Array 2
    Depositor
    Randall Platt
    Article
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184738-plasmid-map-sequence-id-361531

    Enlarge

  11. EGFP-uTEV1-MAVS-mTA4

    Plasmid
    #171002
    Purpose
    HiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)
    Depositor
    Alice Ting
    Article
    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:
    Insert
    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Mutation
    MAVS(tmd)[Y536delinsFIVLI]
    Promoter
    pTRE-tight (TetOn)
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    171002-plasmid-map-sequence-id-335829

    Enlarge

  12. pRS405 SEC66-VAC8-RFP

    Plasmid
    #166843
    Purpose
    Expresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.
    Depositor
    Daniel Klionsky
    Article
    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554
    Insert
    VAC8 (VAC8, SEC66 Budding Yeast)
    Tags
    RFP and SEC66 transmembrane domain
    Expression
    Yeast
    Mutation
    SEC66 transmembrane domain
    Promoter
    VAC8 promoter
    Available Since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166843-plasmid-map-sequence-id-329331

    Enlarge

  13. pFS_1064_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23106-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid
    #184739
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23106
    Depositor
    Randall Platt
    Article
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184739-plasmid-map-sequence-id-361530

    Enlarge

  14. pDRF'- AmTryoshka1;3-LS-F138I-T78H

    Plasmid
    #100963
    Purpose
    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    AmTryoshka1;3-LS-F138I-T78H (AMT1;3 Mustard Weed)
    Expression
    Yeast
    Mutation
    F138I, T78H
    Promoter
    PMA
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100963-plasmid-map-sequence-id-193742

    Enlarge

  19. pFS_1112_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23109-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid
    #184740
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23109
    Depositor
    Randall Platt
    Article
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184740-plasmid-map-sequence-id-361528

    Enlarge

Showing: 1921 - 1940 of 55028 results