We narrowed to 22,079 results for: his

1. pAc5.1B-lambdaN-HA-DmTral-LSmTex-del46-49-V5His6_B

   Plasmid

   #145995

   Purpose

   Insect Expression of DmTral-LSmTex-del46-49

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSmTex-del46-49 (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAc5.1B-EGFP-DmTral-LSm-E23K-V5His6_B

   Plasmid

   #145983

   Purpose

   Insect Expression of DmTral-LSm-E23K

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSm-E23K (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation A198C compared to the sequenc…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAc5.1B-EGFP-DmTral-LSm-R21E-V5His6_B

   Plasmid

   #145984

   Purpose

   Insect Expression of DmTral-LSm-R21E

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSm-R21E (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation A198C compared to the sequenc…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAc5.1B-EGFP-DmTral-LSmTex-del46-49-V5His6_B

   Plasmid

   #145985

   Purpose

   Insect Expression of DmTral-LSmTex-del46-49

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSmTex-del46-49 (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAc5.1B-lambdaN-HA-DmTral-LSm-E23K-V5His6_B

   Plasmid

   #145990

   Purpose

   Insect Expression of DmTral-LSm-E23K

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSm-E23K (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation A198C compared to the sequenc…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAc5.1B-lambdaN-HA-DmTral-LSm-R21ER42EF44A-V5His6_B

   Plasmid

   #145991

   Purpose

   Insect Expression of DmTral-LSm-R21ER42EF44A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSm-R21ER42EF44A (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation A198C compared to the sequenc…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAc5.1B-lambdaN-HA-DmTral-LSm-R21ES13AI15A-V5His6_B

   Plasmid

   #145992

   Purpose

   Insect Expression of DmTral-LSm-R21ES13AI15A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSm-R21ES13AI15A (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation A198C compared to the sequenc…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1B-lambdaN-HA-DmTral-LSm-R21E-V5His6_B

   Plasmid

   #145993

   Purpose

   Insect Expression of DmTral-LSm-R21E

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSm-R21E (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation A198C compared to the sequenc…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAc5.1B-lambdaN-HA-DmTral-LSm-R42E-V5His6_B

   Plasmid

   #145994

   Purpose

   Insect Expression of DmTral-LSm-R42E

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSm-R42E (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation A198C compared to the sequenc…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAc5.1B-lambdaN-HA-Dm4E-T_1-510-V5His6_D

    Plasmid

    #146151

    Purpose

    Insect Expression of Dm4E-T_1-510

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    Dm4E-T_1-510 (4E-T Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAc5.1B-EGFP-Dm4E-T_1-510-V5His6_D

    Plasmid

    #146149

    Purpose

    Insect Expression of Dm4E-T_1-510

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    Dm4E-T_1-510 (4E-T Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23K-V5His6_C

    Plasmid

    #146073

    Purpose

    Insect Expression of DmTral-LSm-R21EE23K

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSm-R21EE23K (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A198C compared to the sequenc…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAc5.1B-lambdaN-HA-DmTral-LSm-R21EF44A-V5His6_C

    Plasmid

    #146074

    Purpose

    Insect Expression of DmTral-LSm-R21EF44A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSm-R21EF44A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A198C compared to the sequenc…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1B-lambdaN-HA-DmTral-LSm-R42EF44A-V5His6_C

    Plasmid

    #146075

    Purpose

    Insect Expression of DmTral-LSm-R42EF44A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSm-R42EF44A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A198C compared to the sequenc…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc5.1B-lambdaN-HA-DmTral-LSm-S13AI15A-V5His6_C

    Plasmid

    #146076

    Purpose

    Insect Expression of DmTral-LSm-S13AI15A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSm-S13AI15A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A198C compared to the sequenc…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1B-lambdaN-HA-DmTral-LSm-S70AD71K-V5His6_C

    Plasmid

    #146077

    Purpose

    Insect Expression of DmTral-LSm-S70AD71K

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSm-S70AD71K (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A198C compared to the sequenc…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1B-EGFP-DmTral-LSm-F44A-V5His6_C

    Plasmid

    #146047

    Purpose

    Insect Expression of DmTral-LSm-F44A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSm-F44A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A198C compared to the sequenc…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc5.1B-EGFP-DmTral-LSm-R21EE23KR42EF44A-V5His6_C

    Plasmid

    #146048

    Purpose

    Insect Expression of DmTral-LSm-R21EE23KR42EF44A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSm-R21EE23KR42EF44A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A198C compared to the sequenc…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-EGFP-DmTral-LSm-R21EE23K-V5His6_C

    Plasmid

    #146050

    Purpose

    Insect Expression of DmTral-LSm-R21EE23K

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSm-R21EE23K (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A198C compared to the sequenc…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc5.1B-EGFP-DmTral-LSm-R21EF44A-V5His6_C

    Plasmid

    #146051

    Purpose

    Insect Expression of DmTral-LSm-R21EF44A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSm-R21EF44A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A198C compared to the sequenc…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAc5.1B-EGFP-DmTral-LSm-R21ER42EF44A-V5His6_C

    Plasmid

    #146052

    Purpose

    Insect Expression of DmTral-LSm-R21ER42EF44A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSm-R21ER42EF44A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A198C compared to the sequenc…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAc5.1B-EGFP-DmTral-LSm-R21ES13AI15A-V5His6_C

    Plasmid

    #146053

    Purpose

    Insect Expression of DmTral-LSm-R21ES13AI15A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSm-R21ES13AI15A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A198C compared to the sequenc…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAc5.1B-EGFP-DmTral-LSm-R42EF44A-V5His6_C

    Plasmid

    #146054

    Purpose

    Insect Expression of DmTral-LSm-R42EF44A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSm-R42EF44A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A198C compared to the sequenc…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAc5.1B-EGFP-DmTral-LSm-S13AI15A-V5His6_C

    Plasmid

    #146055

    Purpose

    Insect Expression of DmTral-LSm-S13AI15A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSm-S13AI15A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A198C compared to the sequenc…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAc5.1B-lambdaN-HA-DmTral-LSm-D30KT35A-V5His6_C

    Plasmid

    #146067

    Purpose

    Insect Expression of DmTral-LSm-D30KT35A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSm-D30KT35A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A198C compared to the sequenc…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAc5.1B-lambdaN-HA-DmTral-LSm-del46-60-V5His6_C

    Plasmid

    #146068

    Purpose

    Insect Expression of DmTral-LSm-del46-60

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSm-del46-60 (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAc5.1B-lambdaN-HA-DmTral-LSm-F44A-V5His6_C

    Plasmid

    #146069

    Purpose

    Insect Expression of DmTral-LSm-F44A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSm-F44A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A198C compared to the sequenc…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAc5.1B-lambdaN-HA-DmTral-LSm-K73AD74A-V5His6_C

    Plasmid

    #146070

    Purpose

    Insect Expression of DmTral-LSm-K73AD74A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSm-K73AD74A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A198C compared to the sequenc…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KS13AI15A-V5His6_C

    Plasmid

    #146072

    Purpose

    Insect Expression of DmTral-LSm-R21EE23KS13AI15A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSm-R21EE23KS13AI15A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A198C compared to the sequenc…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAc5.1B-EGFP-DmTral-LSm-del46-60-V5His6_C

    Plasmid

    #146046

    Purpose

    Insect Expression of DmTral-LSm-del46-60

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSm-del46-60 (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAc5.1B-EGFP-DmSmaug-del584-762-V5His6_C

    Plasmid

    #146006

    Purpose

    Insect Expression of DmSmaug-del584-762

    Depositor

    Elisa Izaurralde

    Insert

    DmSmaug-del584-762 (smg Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    7 silent mutations and a deletion of the AA 961-9…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAc5.1B-lambdaN-HA-DmSmaug-del_584-762-V5His6_C

    Plasmid

    #146015

    Purpose

    Insect Expression of DmSmaug-del584-762

    Depositor

    Elisa Izaurralde

    Insert

    DmSmaug-del584-762 (smg Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    7 silent mutations and a deletion of the AA 961-9…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAc5.1B-lambdaN-HA-DmUPF3-K31E-siRNAres-V5His6_N

    Plasmid

    #147007

    Purpose

    Insect Expression of DmUPF3-K31E-siRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmUPF3-K31E-siRNAres (Upf3 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    5 silent and one non silent mutation P305S compar…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAc5.1B-lambdaN-HA-DmUPF1_92-1180-siRNAres-V5His6_N

    Plasmid

    #146990

    Purpose

    Insect Expression of DmUPF1_92-1180-siRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmUPF1_92-1180-siRNAres (Upf1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    1 non silent mutations: G995E compared to the seq…

    Promoter

    Available since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAc5.1B-lambdaN-HA-DmCup_1-417-Y342AL347A-V5His6_M

    Plasmid

    #146901

    Purpose

    Insect Expression of DmCup_1-417-Y342AL347A

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

    Insert

    DmCup_1-417-Y342AL347A (cup Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pACEBac1-gamma-tubulin(N229A)-TEV-His6

    Plasmid

    #178077

    Purpose

    Insect cell expression of gamma-tubulin(N229A)-TEV-His6

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    gamma-tubulin with an N229A point mutation (TUBG1 Human)
    

    Use

    Tags

    TEV-His6

    Expression

    Insect

    Mutation

    Changed Asparagine 229 to Alanine

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. 6xHis(TEV)-Drp1(K569A,K571A)-StrepII

    Plasmid

    #174426

    Purpose

    Expresses human Drp1 Isoform 3 with K569A, K571A mutations in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Use

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    K569A, K571A

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. HisGB1-Fusion + CBP TAZ1(340-439)

    Plasmid

    #173762

    Purpose

    Bacterial coexpression of CBP TAZ1 and CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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39. HisGB1-FusionL21A + CBP TAZ1(340-439)

    Plasmid

    #173763

    Purpose

    Bacterial Coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246L231A):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. HIsGB1-FusionL63A + CBP TAZ1(340-439)

    Plasmid

    #173764

    Purpose

    Bacterial coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246):Hif1a(796-826L822A) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

    Plasmid

    #176573

    Purpose

    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Use

    Tags

    Avitag, Hexahistidine tag, and mEGFP

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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42. pET29b-mVenus-dspB(E184Q W330Y)-6xHis

    Plasmid

    #176577

    Purpose

    Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Use

    Tags

    Hexahistidine tag and mVenus

    Expression

    Bacterial

    Mutation

    E184Q W330Y

    Promoter

    T7

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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43. 6xHis(TEV)-Drp1(K557A,K560A)-StrepII

    Plasmid

    #174425

    Purpose

    Expresses human Drp1 Isoform 3 with K557A, K560A mutations in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Use

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    K557A, K560A

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET28a-anticalin-FLN-ELP-His-ybbr

    Plasmid

    #168039

    Purpose

    Expresses anticalin targeting CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    3xELP linker, 6x Histag, ddFLN4 (C18S), and ybbr …

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. pET28a-SdrG-FLN-ELP-His-ybbr

    Plasmid

    #168047

    Purpose

    Expresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    

    Use

    Tags

    3xELP linker, 6x Histag, ddFLN4, and ybbr tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KR42EF44A-V5His6_C

    Plasmid

    #146071

    Purpose

    Insect Expression of DmTral-LSmR21EE23KR42EF44A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSmR21EE23KR42EF44A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    R21E, E23K, R42E, F44A. Additionally, one silent …

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C

    Plasmid

    #146049

    Purpose

    Insect Expression of DmTral-LSmR21EE23KS13AI15A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSmR21EE23KS13AI15A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    S13A, I15A, R21E, E23K, one silent mutation A198C…

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCu-His6-Atg13[571–700]-Ss(424)

    Plasmid

    #166852

    Purpose

    Expresses 6xHis tagged ATG13 [571–700] fragment with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast.

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

    Insert

    Atg13[571–700] (ATG13 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    pCu

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. pQE80L-His-SpyTag-XMod(XL2V)-Doc

    Plasmid

    #157676

    Purpose

    Expression of XMod-Doc mutant XL2V (All LEUs were mutated to VAL in XMod region) with His6x tag and SpyTag.

    Depositor

    Michael Nash

    Article

    Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

    Insert

    XMod-Doc mutant XL2V with Histag and SpyTag
    

    Use

    Tags

    Histag and SpyTag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. T7pr-His6-MBP-TEV-Cas9-BirA*

    Plasmid

    #159993

    Purpose

    Bacterial expression of Cas9-BirA*

    Depositor

    Jacob Corn

    Article

    Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

    Insert

    Cas9-BirA*
    

    Use

    Tags

    6X-His, MBP, 3X-FLAG, NLS

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    T7

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge