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Plasmid#141069PurposeFluorescent biosensor for 2GSH/GSSG ratio with mitochondrial localizationDepositorInsertGrx1-roCherry
UseTagsdMTSExpressionMammalianMutationChanged Alanine 150 to Cysteine, inserted Threoni…PromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS0812 hU6-BpiI-OgeuIscB omegaRNA scaffold
Plasmid#176541Purposehuman U6-driven expression of OgeuIscB omegaRNA scaffold with BpiI site for guide cloningDepositorInsertOgeuIscB omegaRNA scaffold
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP Lamp1-Venus
Plasmid#89937PurposeExpresses Lamp1 tagged with Venus in mammalian cellsDepositorInsertrat Lamp1 (Lamp1 Rat)
UseRetroviralTagsVenusExpressionMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS133
Plasmid#211504PurposeAAV genome to express mTagBF2 under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seqDepositorInsertmTagBF2
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
MBC2-null
Plasmid#162749PurposeMSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genesDepositorInsertmurine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBC1-null
Plasmid#162748PurposeMSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genesDepositorInsertmurine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS134
Plasmid#211505PurposeAAV genome to express Venus under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seqDepositorInsertVenus
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS132
Plasmid#211503PurposeAAV genome to express mCherry under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seqDepositorInsertmCherry
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDF0317 HvsCas7-11 mammalian expression
Plasmid#172511PurposeEncodes human codon optimized HvsCas7-11 protein alone for expression in mammalian cellsDepositorInsertHvsCas7-11 mammalian expression
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry
Plasmid#191500PurposeGROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eyeDepositorInsertCaSR-EGFP-SV40
UseCreation of transgenic fish using tol2 transgenes…TagsExpressionMutationPromotermyo6bAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IB-mGFP-FTH1
Plasmid#192800PurposeStable expression of mGFP-FTH1 in mammalian cellsDepositorInsertFTH1 (FTH1 Human)
UseTagsmonomeric EGFP (A206K)ExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pAAV.CAG.Flex.Twitch2B.WPRE.SV40
Plasmid#100039PurposeAAV expression of FLEXed Twitch2B driven by CAG promoter. Twitch 2B is a fluorescent reporter for calcium signalingDepositorInsertTwitch2B
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGST2-GST-TEV-OPTN
Plasmid#198428PurposeUsed for the expression and purification of OPTN (WT) (SMC Internal No. 1587)DepositorInsertoptineurin (OPTN Human)
UseTagsGST and TEVExpressionBacterialMutationPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB
Plasmid#176587PurposeBacterial expression of His14-MBP-TEV-AmaTnpBDepositorInsertsMBP tag
TnpB
UseTagsHis14 tagExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
MacroH2A1.2-c002
Plasmid#173088PurposeExpression of recombinant fusion protein in E. coliDepositorInsertMACROH2A1 (MACROH2A1 Human)
UseTagsmCeruleanExpressionBacterialMutationQ162-N372, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer
Plasmid#172501PurposeEncodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacersDepositorInsertDisCas7-11 full locus with Golden gate acceptor site for spacer
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
MacroH2A1.1-c002
Plasmid#173087PurposeExpression of recombinant fusion protein in E. coliDepositorInsertMACROH2A1 (MACROH2A1 Human)
UseTagsmCeruleanExpressionBacterialMutationQ162-N369, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBMN HA-NDP52
Plasmid#199307PurposeFor expression of the mammalian NDP52 tagged with HADepositorInsertNDP52 (CALCOCO2 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only