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pBigTB

Plasmid

#149432

Purpose

shuttle vector with blasticidin-S resistance into ROSA26 targetting vectors

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Type

Empty backbone

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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DS-ST1cas

Plasmid

#48647

Purpose

Bacterial S. thermophilus #1 Cas9 (ST1) + tracrRNA expression, cloDF13/spectinomycin

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
ST1 tracrRNA

Use

CRISPR

Promoter

proC

Available Since

Nov. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pBbA6k-RFP

Plasmid

#35291

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

LlacO-1

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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p413GPD-hTPI

Plasmid

#50719

Purpose

expression of human TPI1 in yeast

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Expression

Yeast

Promoter

GPD

Available Since

Jan. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST26-hOPTN-D474N

Plasmid

#23051

Depositor

Jon Ashwell

Article

Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43.

Insert

Optineurin (OPTN Human)

Tags

His

Expression

Mammalian

Mutation

D474N

Available Since

March 16, 2010

Availability

Academic Institutions and Nonprofits only
pColE1_sgRNA_pos9_AmpR

Plasmid

#119152

Purpose

Encodes sgRNA targeting position 9 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 9 in pColE1_70a_deGFP_KanR

Use

Crispr

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pBbE1k-RFP

Plasmid

#35333

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Trc

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-act5cB-GAL4QF

Plasmid

#61309

Purpose

Expresses chimeric transactivator GAL4_DBD:QF2w_AD in Drosophila

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

GAL4_DBD::QF2w_AD

Expression

Insect

Mutation

Changed EK on C-terminus of QF2 to KKKK

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGL001

Plasmid

#115674

Purpose

Fluorescent reporter for E.coli K12 fliC promoter activity

Depositor

Gene-Wei Li

Article

Parker et al J Bacteriol. 2019 Sep 6;201(19). pii: JB

Insert

fliC promoter fused with GFP

Tags

Green fluorescent protein (GFP)

Expression

Bacterial

Promoter

fliC promoter

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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GFP Q157TAG

Plasmid

#68297

Purpose

Q157TAG GFP reporter

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

GFP Q157TAG

Tags

His

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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