161,152 results

1. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

   Plasmid

   #202553

   Purpose

   Nontargeting control vector with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Expression

   Mutation

   Puromycin-resistance gene in the pLV hU6-sgRNA hU…

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEVA63_HelperΔgIII

   Plasmid

   #190433

   Purpose

   M13 helper genes cloned into the pSEVA631 vector, except the minor coat protein coding gene gIII (also called gp3, or g3p). This plasmid does not carry an M13 packaging signal.

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Insert

   M13 genes I-V and VII-XI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF2-FL-hMer_gR1

   Plasmid

   #225993

   Purpose

   Expresses a chimeric contruct having N-terminal human MerTK and C-terminal human Interferon Gamma Receptor

   Depositor

   Sergei Kotenko, Raymond Birge

   Article

   Tsou et al J Biol Chem. 2014 Sep 12;289(37):25750-6

   Insert

   human MerTK N-Terminal human Interferon Gamma R1 Receptor C-Terminal fusion (MERTK, IFNGR1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28b-ptetO::bac::gfp

   Plasmid

   #217882

   Purpose

   expresses the bac-BGC in bacterial cells, produces bacillamide D

   Depositor

   Tobias A. M. Gulder

   Article

   Hohmann et al Commun Biol. 2024 May 28;7(1):655. doi:

   Inserts

   Dehydrogenase
   Non-ribosomal peptide synthetase
   Aminotransferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ptetO

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL

   Plasmid

   #216253

   Purpose

   FRET-based calcium sensor located in the ER lumen, Tet-inducible

   Depositor

   Edward Avezov

   Article

   Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

   Insert

   ER-targeted D4 second generation cameleon (FRET calcium sensor)
   

   Use

   Lentiviral

   Tags

   REAch and mTurquoise

   Expression

   Mutation

   Promoter

   TRE3G

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRDB_118

   Plasmid

   #216089

   Purpose

   Cas9 [Sp] CRISPRa targeting CD47, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD47 guide
   

   Use

   CRISPR and Lentiviral; Positive controls

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2/shCol1a1/GFP4_Seq1.3

   Plasmid

   #206136

   Purpose

   Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Article

   Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

   Insert

   COL1A1 (Col1a1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT2/NT/GFP4

   Plasmid

   #206133

   Purpose

   Non-targeting shRNA control

   Depositor

   Maria Castro

   Insert

   Mammalian Non-Target
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7-EGFP-C1-HIV-Rev_O

   Plasmid

   #147113

   Purpose

   Mammalian Expression of HIV-Rev

   Depositor

   Elisa Izaurralde

   Article

   Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

   Insert

   HIV-Rev
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. H1.224.M.luc

    Plasmid

    #193705

    Purpose

    Expresses a truncated PolIII H1 promoter of 224 nucleotides (H1.224) with the TATA box mutated

    Depositor

    Elena Herrera-Carrillo

    Article

    Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

    Insert

    Truncated PolIII H1 promoter (H1.224) with the TATA box mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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