178,101 results
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Plasmid#80147PurposeGCaMP6 is a genetically encoded fluorescent Ca2+ sensor . TPC2-GCaMP6 allows the visualization of Ca2+ release from intracellular Ca2+ stores in real timeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTPCN2 (TPCN2 Human)
TagsGCaMP6mExpressionMammalianPromoterCMVAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGWB402-omega
Plasmid#74797PurposeGateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.DepositorTypeEmpty backboneExpressionPlantPromoter2xCaMV35S enhancer and omegaAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHDE-35S-Cas9-mCherry-UBQ
Plasmid#78932PurposeFor CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants. Enable production of two gRNAsDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCIP
Plasmid#79009PurposeFor expression in mouse ESCs or other eukaryotic cells. Contains pCAGGS-IRES-Puro-bpA, with a NheI-MfeI cloning site in between pCAGGS and IRES.DepositorTypeEmpty backboneExpressionMammalianPromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Synapsin sHRPa-NRX in FSW lentiviral vector
Plasmid#73147PurposeLarge fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.DepositorInsertsHRPa-NRX
UseLentiviralTags2x HA tag and Flag tagExpressionMammalianPromoterSynapsinAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG sHRPb-NLG
Plasmid#73144PurposeSmall fragment of split HRP fused to the extracellular terminus of NLG1. CAG promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsHRPb-NLG (Nlgn1 Synthetic, Mouse)
TagsV5 tagExpressionMammalianPromoterCAGAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUCP20T-ecfp
Plasmid#78463PurposeBroad range bacterial fluorescence labelingDepositorInserteCFP
ExpressionBacterialAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cytosolic FAK biosensor
Plasmid#78300PurposeFRET biosensor. Visualization of cytosolic FAK activity in live cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCytosolic FAK biosensor
ExpressionMammalianPromoterCMVAvailable sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lyn FAK biosensor
Plasmid#78299PurposeFRET biosensor. Visualization of FAK activity at lipid rafts in live cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLyn Fak biosensor
ExpressionMammalianPromoterCMVAvailable sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRubiC-T2A-Cas9
Plasmid#75347PurposeUbiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.DepositorInsertCas9
UseCRISPR and RetroviralTagsmCherry (via t2a)ExpressionMammalianPromoterUbiquitinAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only