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62,262 results

Showing: 9871 - 9880 of 62262 results

  3. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

    Plasmid
    #71828
    Purpose
    Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    BACH2-sgRNA8 (BACH2 Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    U6
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71828-plasmid-map-sequence-id-131023

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  4. R777-E235 Hs.RASAL2

    Plasmid
    #70519
    Purpose
    Gateway ORF clone of human RASAL2 [NM_170692.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASAL2 (RASAL2 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70519-plasmid-map-sequence-id-125968

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  5. R777-E239 Hs.RASGRF1

    Plasmid
    #70523
    Purpose
    Gateway ORF clone of human RASGRF1 [NM_001145648.1] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASGRF1 (RASGRF1 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70523-plasmid-map-sequence-id-125972

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  6. R777-E117 Hs.KSR1

    Plasmid
    #70401
    Purpose
    Gateway ORF clone of human KSR1 [NM_014238.1] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    KSR1 (KSR1 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70401-plasmid-map-sequence-id-125850

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  7. R777-E063 Hs.ETS1

    Plasmid
    #70347
    Purpose
    Gateway ORF clone of human ETS1 [NM_005238.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ETS1 (ETS1 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70347-plasmid-map-sequence-id-125796

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  8. PMXS-GOT1

    Plasmid
    #72872
    Purpose
    The retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly.
    Depositor
    David Sabatini
    Article
    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10
    Insert
    GOT1 (GOT1 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    sgRNA resistant human GOT1, 7 silent mutations c1…
    Available Since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 9871 - 9880 of 62262 results