Addgene

We narrowed to 11,638 results for: pcdna

Showing: 2941 - 2970 of 11638 results

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pCDNA3 Flag MKK7b2

Plasmid

#14623

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7b2 (Map2k7 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL (AA)

Plasmid

#24240

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(AA) (BCL2L11 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Threonine 56 to Alanine changed Serine …

Promoter

Available since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-PKCZeta KR HA Tagged

Plasmid

#24608

Depositor

Jeff Wrana

Article

Wang et al Science. 2003 Dec 5. 302(5651):1775-9.

Insert

PKCZeta (Prkcz Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

K281R mutation in the ATP binding domain causing …

Promoter

Available since

June 7, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pY013 (pcDNA3.1-hEeCpf1)

Plasmid

#69985

Purpose

Expresses humanized EeCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hEeCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-Strep/HA-UBE2QL1

Plasmid

#124665

Purpose

Expresses UBE2QL1 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Use

Tags

2xStrep/HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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ciCas9(L22)_pcDNA5

Plasmid

#100551

Purpose

Expresses ciCas9(L22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

ciCas9(L22)

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-P2X2-CFP

Plasmid

#22401

Depositor

Henry Lester

Article

Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

Insert

ATP-gated P2X channel 2 (P2rx2 Rat)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-nAChr-Alpha6

Plasmid

#48812

Purpose

this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

Depositor

Henry Lester

Article

Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

Insert

nAChr-alpha6 (Chrna6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HSPA14

Plasmid

#19461

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

HSPA14 (HSPA14 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Granulin 2-no linker

Plasmid

#176921

Purpose

express Granulin 2 without linker 3 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 2 (GRN Human)

Use

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Granulin 1+linker2

Plasmid

#176918

Purpose

express Granulin 1 with linker 2 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 1+linker2 (GRN Human)

Use

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nsp1

Plasmid

#157680

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp1 (ORF1ab SARS-CoV-2)

Use

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-Beta2-PAmcherry1

Plasmid

#114190

Purpose

studying clustering of Beta2 receptors by superresolution microscopy (PALM)

Depositor

Aleksandra Radenovic

Insert

Adrenergic receptor beta 2 (ADRB2 Human)

Use

Tags

3HA, FLAG, and PAmcherry1

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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HMGN1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176943

Purpose

Mammalian expression vector encoding HMGN1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

HMGN1 (HMGN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Cx43-Myc

Plasmid

#60693

Purpose

mammalian expression of Myc-tagged rat Cx43

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
ORF31-2xStrep pCDNA4

Plasmid

#129744

Purpose

Expresses KSHV ORF31 with Cterm 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF31 (ORF31 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Tags

2xStrep Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean-WW-NES

Plasmid

#84629

Purpose

Encodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

Cerulean-WW-NES

Use

Tags

6xHis, Cerulean, Nuclear export signal (NES), T7 …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Paxillin/pcDNA3

Plasmid

#67775

Purpose

Paxilin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

Paxillin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-CARD14-G117S

Plasmid

#69920

Purpose

Expresses mutant FLAG-CARD14 in mammalian cells

Depositor

Francesca Capon

Article

Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

Insert

CARD14 isoform 3 (CARD14 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

G117S

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) eGFP PPP1R8 G4

Plasmid

#129021

Purpose

Expresses an eGFP mRNA with the PPP1R8 G-quadruplex (G4) region in the 3' UTR

Depositor

Jeremy Wilusz

Article

Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) eGFP EML2 G4

Plasmid

#129023

Purpose

Expresses an eGFP mRNA with the EML2 G-quadruplex (G4) region in the 3' UTR

Depositor

Jeremy Wilusz

Article

Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-hMcl-1(T163A)

Plasmid

#25376

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

hMCL1 (MCL1 Human)

Use

Tags

V5 and His tag

Expression

Mammalian

Mutation

changed threonine 163 to alanine

Promoter

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO DNAJA1

Plasmid

#19464

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DNAJA1 (DNAJA1 Human)

Use

Tags

Expression

Mammalian

Mutation

D244G

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO DNAJB6a

Plasmid

#19475

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DNAJB6a (DNAJB6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pY019 (pcDNA3.1-hPmCpf1)

Plasmid

#69991

Purpose

Expresses humanized PmCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hPmCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pY009 (pcDNA3.1-hSsCpf1)

Plasmid

#69981

Purpose

Expresses humanized SsCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hSsCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3 14 mut B-Myb

Plasmid

#25967

Depositor

Rob Lewis

Article

Johnson et al J Biol Chem. 2002 Feb 8. 277(6):4088-97.

Insert

B-Myb (Mybl2 Mouse)

Use

Tags

Flag

Expression

Bacterial

Mutation

Changed threonines 267, 408, 443, 447 490, 497, 5…

Promoter

Available since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-sACE2v1-Fc(IgA1)

Plasmid

#145155

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 1

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Fc region of human IgA1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-deltaA1-VWF

Plasmid

#124823

Purpose

Expresses multimeric human VWF protein without A1 domain. This construct has no tag.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF without A1 domain (VWF Human)

Use

Tags

Expression

Mammalian

Mutation

Deleted A1 domain

Promoter

CMV

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-Green Falcan3

Plasmid

#199706

Purpose

green fluorescent indicator for cAMP imaging

Depositor

Tetsuya Kitaguchi

Article

Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

Insert

Green Falcan3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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