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pLenti6.3_HES7
Plasmid#124075PurposeHES7 with normal stop codonDepositorInsertHES7 (HES7 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
4LD6
Plasmid#60088PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPWWP2B:JMC079-A09:C228600 (PWWP2B Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti4/V5-DEST-P85(R574fs)
Plasmid#40227DepositorInsertP85(R574fs) (PIK3R1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationIntroduced frame shift mutation at amino acid 574PromoterCMVAvailable sinceOct. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
Plekhm1- PH2 pGEX 4T2-GST
Plasmid#73834PurposeExpress human Plekhm1 (PH2 domain) in bacterial cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTagsGstExpressionBacterialMutationto vector was cloned PH2 domain of human Plekhm1…PromoterAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Plekhm1- PH1 pGEX 4T2-GST
Plasmid#73833PurposeExpress human Plekhm1 (PH1 domain) in bacterial cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTagsGstExpressionBacterialMutationto vector was cloned PH1 domain of human Plekhm1…PromoterAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Plekhm1 -C101 pGEX 4T2-GST
Plasmid#73832PurposeExpress human Plekhm1 (S956 to end domain) in bacterial cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTagsGstExpressionBacterialMutationto vector was cloned 101 aa from human Plekhm1 (S…PromoterAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
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4JXT B
Plasmid#60106PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRPRD1B:DCC020-G07:C222611 (RPRD2 Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
4MYJ
Plasmid#60104PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertPF14_0346:MAC04G-A11:C228843
UseBaculovirus expressionTags6xHis-TEV and MHHHHHHSSGRENLYFQGExpressionMutationSee commentsPromoterPolyhedrinAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pTALE64
Plasmid#35189PurposeGolden Gate Compatible TALE Transcription Factor using VP64 DomainDepositorInsertTranscription Activator Like Effector Transcription Factor
UseTALENTagsExpressionMammalianMutation+63 C Terminus, VP64 DomainPromoterCMVAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
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Flag-TRIM24-F869D/F979A/N980A shRescue
Plasmid#28145DepositorInsertTRIM24 (TRIM24 Human)
UseTagsFLAGX2ExpressionMammalianMutationF869D, F979A, N980A and aa 308 and 309 mutated fo…PromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBOBI-hPLD4-flag
Plasmid#222641Purposelentiviral vector for full length human PLD4 expression (with flag tag)DepositorInsertPLD4 (PLD4 Human)
UseLentiviralTagsflagExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
LLhKdgR-pHG165a 2mut
Plasmid#220270PurposeConstitutive expression of a chimeric LacI:KdgR transcription repressorDepositorInsertLLhKdgR
UseTagsExpressionBacterialMutationV52A/Q55VPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
LLhRafR-pHG165a G62A
Plasmid#220273PurposeConstitutive expression of a chimeric LacI:RafR transcription repressorDepositorInsertLLhRafR
UseTagsExpressionBacterialMutationG62APromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
LLhRafR-pHG165a 3mut
Plasmid#220274PurposeConstitutive expression of a chimeric LacI:RafR transcription repressorDepositorInsertLLhRafR
UseTagsExpressionBacterialMutationV52N/Q55L/Q62HPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
LLhSalR-pHG165a 3mut
Plasmid#220276PurposeConstitutive expression of a chimeric LacI:SalR transcription repressorDepositorInsertLLhSalR
UseTagsExpressionBacterialMutationV52L/Q55G/Y62RPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
LLhScrR-pHG165a 3mut
Plasmid#220278PurposeConstitutive expression of a chimeric LacI:ScrR transcription repressorDepositorInsertLLhScrR
UseTagsExpressionBacterialMutationV52I/Q62S/G120SPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
EP003: CAG::GEM7-HaloTag
Plasmid#209303PurposeExpression plasmid for GEM7 (encapsulin from R. rubrum) with C-terminal HaloTag suitable for expression in mice via in utero electroporationDepositorInsertGEM7
UseAAVTagsHaloTagExpressionMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EP004: pAAV TRE::GEM7-HaloTag
Plasmid#209304PurposeDoxycycline-gated expression plasmid (TetOFF) for GEM7 (encapsulin from R. rubrum) with C-terminal HaloTag suitable for expression in miceDepositorInsertGEM7
UseAAVTagsHaloTagExpressionMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pAG Retro EGFP Neo
Plasmid#234464PurposeControl retroviral vector expressing Green Fluorescent Protein. Neomycin selection.DepositorInsertGreen Fluorescent Protein
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMarch 21, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS2-zH2B-GFP
Plasmid#226561PurposeExpress H2B-GFP in zebrafish.DepositorInserth2b (h2bc1 Zebrafish)
UseSynthetic Biology; Zebrafish expressionTagsEGFPExpressionMutationPromoterAvailable sinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP1
Plasmid#233685PurposeEngineered SUMO protease expression plasmid designed for efficient protease-mediated elution.DepositorInsertUlp1-like SUMO protease
UseTagsCAHS-107838 and HisTagExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZP05
Plasmid#232319PurposepZP05 was constructed by cloning the oriFn-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, facilitating replication in Fusobacterium nucleatumDepositorInsertOri-repA
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
3'dmDR-CMV-CasRx-bGHpolyA
Plasmid#229556PurposeExpresses CasRx (RfxCas13d) with double processed direct repeats (15nt) and a non-target spacer in between at 3' endDepositorInsertCasRx
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
1203U-U6-gRNA-pCMV-wtCas9-NG-P2A-mcherry-3'dmDR
Plasmid#221448PurposeExpresses wtCas9-NG with double processed direct repeats (15nt) and a non-target spacer in between at 3' end, mCherry tag, and the gRNA targeting HEK293T-site3DepositorInsertswtCas9-NG
mCherry
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLaG2-T7RNAPC
Plasmid#216337PurposeArabinose-inducible PBad promoter driving expression of the anti-eGFP nanobody LaG2 translationally fused to the C-terminal portion (residues 180-883) of T7RNAPDepositorInsertLaG2-T7RNAPC fusion
UseTagsExpressionBacterialMutationg2658a, g3915aPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBS-ACTB-C200-T7
Plasmid#229850PurposeDonor vector for the ACTB site containing T7 sequence for detection of HR products.DepositorInsertPartial sequence of ACTB gene
UseHr donor vector for human actb gene.TagsExpressionMutationPromoterAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCG-scr
Plasmid#229847PurposeExpresses wild-type Cas9 and scrambled gRNA.DepositorInsertguide RNA with scrambled sequence
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Venus_hs_NC_chr1
Plasmid#214682PurposeLentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)DepositorInsertdgRNA_Chr1
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Venus_hs_NC_chr2
Plasmid#214683PurposeLentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 2 (negative control)DepositorInsertdgRNA_Chr2
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Venus_hs_TP73
Plasmid#214685PurposeLentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human TP73DepositorInsertdgRNA_TP73 (TP73 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_BFP_hs_NC_chr1
Plasmid#214686PurposeLentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)DepositorInsertdgRNA_Chr1
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_BFP_hs_TP73
Plasmid#214687PurposeLentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human TP73DepositorInsertdgRNA_TP73 (TP73 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only
