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pCMV-OptoTGFBRs

Plasmid

#118942

Purpose

Mammalian expression plasmid of OptoTGFBRs (optogenetically-activated TGFb receptors)

Depositor

Won Do Heo

Publication

Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

Insert

OptoTGFBRs (Tgfb1, Tgfbr2 Mouse, Mustard Weed)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSV-CaMKIV(1-313)

Plasmid

#45063

Purpose

Expression of constit active CaMKIV

Depositor

Richard Maurer

Publication

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

Cam Kinase IV(1-313) (Camk4 Mouse)

Expression

Mammalian

Mutation

truncated at amino acid 313*

Promoter

RSV

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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D28 Cortactin GFP

Plasmid

#26723

Depositor

Anna Huttenlocher

Publication

Perrin et al Mol Biol Cell. 2006 Jan . 17(1):239-50.

Insert

Cortactin (Cttn Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion removed 28 amino acids (aa# 334-361) enc…

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pCIG PIK3CA E545K

Plasmid

#73055

Purpose

Human PIK3CA E545K

Depositor

Joseph Gleeson

Publication

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

PIK3CA (PIK3CA Human)

Expression

Mammalian

Mutation

E545K

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mApple-Actin-C-18

Plasmid

#54863

Purpose

Localization: Actin, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Publication

Thievessen et al J Cell Biol. 2013 Jul 8;202(1):163-77. d

Insert

Actin (ACTB Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pCMV4Neo-murine IL-17RC(FL)

Plasmid

#46864

Purpose

expresses mouse IL-17RC (all exons)

Depositor

Sarah Gaffen

Publication

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RC (Il17rc Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HIPK1-myc-his

Plasmid

#1850

Depositor

Philip Leder

Publication

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

HIPK1 (Hipk1 Mouse)

Tags

his and myc

Expression

Mammalian

Available since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
pCS2 mouse CV2 PC

Plasmid

#25776

Depositor

Edward De Robertis

Publication

Coffinier et al Mech Dev. 2002 Dec;119 Suppl 1(Suppl 1):

Insert

cross veinless 2 (Bmper Mouse)

Tags

PC

Expression

Mammalian

Available since

July 2, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-dnMCAK

Plasmid

#208650

Purpose

Mammalian expression plasmid expressing eGFP-dnMCAK driven by CMV promoter

Depositor

Samuel Bakhoum

Publication

Li et al Nature. 2023 Aug;620(7976):1080-1088. do

Insert

Kif2c (Kif2c Cricetulus griseus (Chinese hamster))

Tags

eGFP

Expression

Mammalian

Mutation

S92A , S106A , S108A, S112A , S186A, H530A, R534A…

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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HaloTag-WDR5

Plasmid

#200880

Purpose

Mammalian expression of human WDR5 with N-terminal HaloTag fusion

Depositor

Stefan Knapp

Publication

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

HaloTag-WDR5 (WDR5 Human)

Expression

Mammalian

Mutation

WT

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCZGY3196

Plasmid

#172696

Purpose

Prgef-1-gfp1-10 Expresses split GFP parts 1-10 in all C. elegans neurons (only shows active fluorescence when binds to GFP11)

Depositor

Yishi Jin

Publication

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

Prgef-1

Tags

GFP 1-10

Expression

Worm

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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R726-M01-892: His6-Halotag-tev-Hs.RAF1(52-131)

Plasmid

#159696

Purpose

E. coli protein expression of His6-Halotag-tev-Hs.RAF1(52-131)

Depositor

Dominic Esposito

Publication

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-Halotag-tev-Hs.RAF1(52-131) (RAF1 Human)

Tags

His6-Halotag-tev

Expression

Bacterial

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-FOXE1

Plasmid

#153120

Purpose

Expression of Flag tagged human FOXE1 in mammalian cells

Depositor

Stefan Koch

Publication

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXE1 (FOXE1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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Dynein-2_complex

Plasmid

#132536

Purpose

Full length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.

Depositor

Andrew Carter

Publication

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Inserts

DYNC2H1 (DYNC2H1 Human)
DYNC2LI1 (DYNC2LI1 Human)
WDR34 (WDR34 Human)
WDR60 (WDR60 Human)
DYNLL1 (DYNLL1 Human)
DYNLL2 (DYNLL2 Human)
DYNLRB1 (DYNLRB1 )
DYNLRB2 (DYNLRB2 Human)
DYNLT1 (DYNLT1 Human)
TCTEX1D2 (TCTEX1D2 Human)
DYNLT3 (DYNLT3 Human)
LC8-like (LOC392067 Human)

Tags

8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR221_SLC8A3

Plasmid

#132186

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC8A3 (SLC8A3 Human)

Expression

Mammalian

Available since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pHAGE-RAF1

Plasmid

#116785

Purpose

Lentiviral expression of RAF1

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RAF1 (RAF1 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EF1a_LMX1A_P2A_Hygro_Barcode

Plasmid

#120457

Purpose

Barcoded lentiviral vector to express LMX1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

LMX1A (LMX1A Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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alpha5-EGFP

Plasmid

#118956

Purpose

GABAA receptor expression (alpha5 subunit). EGFP tag at the C-terminus.

Depositor

Angel de Blas

Insert

GABRA5 (Gabra5 Rat)

Tags

EGFP

Expression

Mammalian

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt

Plasmid

#85111

Purpose

expression of recombinant human mature (delta19) ETFalpha wt without tag

Depositor

Pal Falnes

Publication

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human mature (delta19) ETFalpha (ETFA Human)

Expression

Bacterial

Promoter

T7

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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ADAMTS5_pCSdest

Plasmid

#53819

Depositor

Roger Reeves

Publication

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ADAMTS5 (ADAMTS5 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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