We narrowed to 13,104 results for: CRISPR-Cas9

1. KA2963_eSpCas9-PGKHygdtkCh

   Plasmid

   #124205

   Purpose

   Expression of eSpCas9(1.1) and sgRNA

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMB1052:miniTol2-U6-(empty)SaCas9_gRNA-CAG-Cre-P2A-NeoR-WPRE

   Plasmid

   #168108

   Purpose

   Tol2 transposon vector of empty U6-S.aureus Cas9 gRNA cassette and CAG-driven Cre-NeoR

   Depositor

   Erich Jarvis

   Article

   Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

   Plasmid

   #87350

   Purpose

   S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Use

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/S355C/C574S/S867C

   Promoter

   T7

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT2K-CAGGS-U6-stuffer-dCAs9-VP160-T2A-IRES-mCherry

   Plasmid

   #114726

   Purpose

   dCas9-VP160 expression in mammalian cells

   Depositor

   Martine Roussel

   Article

   Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

   Insert

   dCas9-VP160
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRI006-pYFAC-riboB-PgpdA-dSpCas9-VPR-TtrpC

   Plasmid

   #140199

   Purpose

   Episomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Insert

   dSpCas9-VPR
   

   Use

   CRISPR and Synthetic Biology; Expression in asper…

   Tags

   VPR (VP64-p65-Rta), NLS

   Expression

   Mutation

   Promoter

   PgpdA

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLD003-pCMV-APOBEC-Cas9(D10A)-rPB(8kD)

   Plasmid

   #165445

   Purpose

   Expresses ACX, rPB(8kD) in mammalian cells

   Depositor

   Wei Leong Chew

   Article

   Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

   Insert

   APOBEC-nCas9-rPB(8kD)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-2)

   Plasmid

   #159660

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-3)

   Plasmid

   #159661

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-1)

   Plasmid

   #159659

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variant

    Plasmid

    #101202

    Purpose

    Bacterial expression plasmid for SpCas9∆REC3 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant M1–N497,GGS,V713–D1368
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    M1–N497, GGS, V713–D1368

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant

    Plasmid

    #101233

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 2 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant G582A/V583A/E584A/D585A/N588A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    G582A, V583A, E584A, D585A and N588A

    Promoter

    T7

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-eCas9-T2A-EGFP-U6-gRNA-no ITR and f1 ori

    Plasmid

    #234724

    Purpose

    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in neurons. The plasmid lacks AAV2 ITR and f1 ori elements, enabling more efficient transfection and expression.

    Depositor

    Kirill Martemyanov

    Article

    Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    T2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDGB3 alpha2 P35S:dCas9:SRDX:Tnos (GB1188)

    Plasmid

    #160608

    Purpose

    TU for the expression of the dCas9 with the repressor domain SRDX as a CT fusion (CRISPR tools)

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    P35s:dCas9:SRDX:tNOS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

    Plasmid

    #199252

    Purpose

    Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    High specificity eSpCas9(1.1)-D10A nickase
    

    Use

    CRISPR

    Tags

    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A K848A K1003A R1060A

    Promoter

    CAG promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT3TS-spCas9-ABE-Umax-rest1

    Plasmid

    #222144

    Purpose

    Adenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKMD111e - intein-Cas9(S219-G521R)

    Plasmid

    #64192

    Purpose

    Expresses intein-Cas9(S219-G521R) in mammalian cells

    Depositor

    David Liu

    Article

    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

    Insert

    mammalian codon-optimized S. pyogenes intein-Cas9(S219-G521R) - NLS - 3x FLAG
    

    Use

    CRISPR

    Tags

    NLS - 3x FLAG (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-Flag-wtSpCas9 (without sgRNA)

    Plasmid

    #92353

    Purpose

    Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFlag-NLS-wild-type SpCas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAVS1_dCas9-XTEN-KRAB-p2A-mCherry

    Plasmid

    #222107

    Purpose

    Donor vector for CRISPRi integration at AAVS1 with an mCherry fluorophore

    Depositor

    Jonathan Weissman

    Article

    She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

    Insert

    CRISPRi-kox1(KRAB) (cas9 S. pyogenes, Human)
    

    Use

    AAV and CRISPR

    Tags

    HA and P2A-mCherry

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1_dCas9-XTEN-KRAB-p2A-GFP

    Plasmid

    #222108

    Purpose

    Donor vector for CRISPRi integration at AAVS1 with a GFP fluorophore

    Depositor

    Jonathan Weissman

    Article

    She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

    Insert

    CRISPRi-kox1(KRAB) (cas9 S. pyogenes, Human)
    

    Use

    AAV and CRISPR

    Tags

    HA and P2A-GFP

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. A3H HapII R175/6E-Cas9n-UGI

    Plasmid

    #119142

    Purpose

    Base editor made from APOBEC3H Haplotype II RNA binding mutant RR175/6EE

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3H Haplotype II RR175/6EE-Cas9 nickase-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    A3H Hap II RR175/6EE

    Promoter

    CMV

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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