We narrowed to 2,230 results for: Kap

1. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

   Plasmid

   #213031

   Purpose

   Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration

   Depositor

   Donna Romero, Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
   

   Use

   Luciferase

   Tags

   Firefly luciferase antisense intron (3' UTR)

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE bidirectional tet-on

   Available since

   July 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

   Plasmid

   #213026

   Purpose

   Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1

   Plasmid

   #213025

   Purpose

   Expresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cells

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   LINE-1 ORF2p (1-1275)
   

   Use

   Tags

   3C-3xFlag (ORF2p)

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A

   Plasmid

   #177335

   Purpose

   bacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinase

   Depositor

   Christopher Overall

   Article

   Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:

   Insert

   SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
   

   Use

   Tags

   Factor Xa site-3xFlag-Myc-6xHis

   Expression

   Bacterial

   Mutation

   silent mutation C to T at position 10546 (elimina…

   Promoter

   T7

   Available since

   Nov. 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213028

   Purpose

   Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Mutation

   E43S D145N double mutant EN- endonuclease catalyt…

   Promoter

   CMV

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213027

   Purpose

   Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Mutation

   D702Y (RT- reverse transcriptase catalytic dead)

   Promoter

   CMV

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213029

   Purpose

   Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. FGFR2 3xFlag

   Plasmid

   #110063

   Purpose

   FGFR2 expression in mammalian cells (JCOPCO paper)

   Depositor

   Martin Fernandez-Zapico

   Article

   Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

   Insert

   fibroblast growth factor receptor 2 (FGFR2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Dec. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. FGFR2 F276C 3xFlag

   Plasmid

   #110064

   Purpose

   FGFR2 F276C expression in mammalian cells (JCOPO paper)

   Depositor

   Martin Fernandez-Zapico

   Article

   Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

   Insert

   fibroblast growth factor receptor 2 (FGFR2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   mutated Phenylalanine 276 to Cysteine (F276C)

   Promoter

   CMV

   Available since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. FGFR2 K41E 3xFlag

    Plasmid

    #110105

    Purpose

    expresses FGFR2 with a single amino acid change in mammalian cells

    Depositor

    Martin Fernandez-Zapico

    Article

    Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

    Insert

    fibroblast growth factor receptor 2 (FGFR2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mutated Lysine 41 to Glutamic acid (K41E)

    Promoter

    CMV

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PA-GFP-α-Tubulin

    Plasmid

    #213900

    Purpose

    Imaging of Tubulin using Photoactivatable GFP

    Depositor

    Lukas Kapitein

    Insert

    PA-GFP-α-Tubulin
    

    Use

    Tags

    Photoactivatable GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET12a-HAL

    Plasmid

    #47097

    Depositor

    Marina Ramirez-Alvarado

    Insert

    HAL
    

    Use

    Tags

    ompT

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET11a-p65(19-325)

    Plasmid

    #44744

    Depositor

    Gourisankar Ghosh

    Insert

    p65 (Rela Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AA 19-325

    Promoter

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pET12a-JOH1

    Plasmid

    #47098

    Depositor

    Marina Ramirez-Alvarado

    Insert

    JOH1 (IGKV3-20 Human)
    

    Use

    Tags

    ompT

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pET12a-DAH

    Plasmid

    #47096

    Depositor

    Marina Ramirez-Alvarado

    Insert

    DAH (IGKV3-20 Human)
    

    Use

    Tags

    ompT

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pET12a-KIo18/08 C214S

    Plasmid

    #47099

    Depositor

    Marina Ramirez-Alvarado

    Insert

    KIo18/08 C214S (IGKV3-20 Human)
    

    Use

    Tags

    ompT

    Expression

    Bacterial

    Mutation

    C214S

    Promoter

    T7

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pET12a-AL-103 delProIns

    Plasmid

    #47095

    Depositor

    Marina Ramirez-Alvarado

    Insert

    AL-103 delProIns (IGKV3-20 Human)
    

    Use

    Tags

    ompT

    Expression

    Bacterial

    Mutation

    delete aa95

    Promoter

    T7

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

18. AbVec_IGKC

    Plasmid

    #183702

    Purpose

    Encode human Ig kappa chain constant region. For cloning variable region and mAb expression.

    Depositor

    David Nemazee

    Insert

    IGKC (IGKC )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS313_MSN5-LY00045

    Plasmid

    #232904

    Purpose

    Plasmid carrying MSN5 from LY00045 (S288C) with its native promoter and terminator.

    Depositor

    Jolanda Van Leeuwen

    Insert

    MSN5 (MSN5 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK637

    Plasmid

    #72437

    Purpose

    Acetobacter aceti 1023 mutS, partial gene

    Depositor

    T. J. Kappock

    Insert

    DNA mismatch repair protein MutS (partial gene)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deletes nt 1-767 and nt 2086-2655 of mutS gene

    Promoter

    n/a

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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