We narrowed to 13,577 results for: ckb

1. pAV10.F3

   Plasmid

   #63199

   Purpose

   BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJT050 lenti pEF-rTetR-LibCloneSite-T2A-mCherry-BSD-WPRE

   Plasmid

   #161925

   Purpose

   Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

   Type

   Empty backbone

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pEF

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI

   Plasmid

   #226009

   Purpose

   DjCas13d guide RNA cloning backbone (lenti)

   Depositor

   Silvana Konermann

   Article

   Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-SauriCas9-KKH-Puro

   Plasmid

   #135966

   Purpose

   Expresses SauriCas9-KKH and puromycin resistance genes, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMY-MCS-P2A-CD90.2

   Plasmid

   #163355

   Purpose

   Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated CD90.2 surface marker

   Depositor

   Teunis Geijtenbeek, Louis Ates

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2TAL3-RR

   Plasmid

   #37276

   Purpose

   TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)

   Depositor

   David Grunwald

   Article

   Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

   Type

   Empty backbone

   Use

   Tags

   C terminal Tal From pTAL3, FokI RR, HA, and N-ter…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMY-MCS-P2A-eGFP

   Plasmid

   #163356

   Purpose

   Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated GFP marker

   Depositor

   Teunis Geijtenbeek, Louis Ates

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKSB-AeU6-3gRNA

   Plasmid

   #183914

   Purpose

   gRNA-expressing plasmid under Aedres aegypti AAEL017763 U6 promoter

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

   Insert

   gRNA scaffold for protospacer cloning into BbsI sites
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Aedes aegypti U6 promoter (AAEL017763)

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI

   Plasmid

   #226011

   Purpose

   CasRx guide RNA cloning backbone (lenti)

   Depositor

   Silvana Konermann

   Article

   Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCfB2904(XI-3 MarkerFree)

    Plasmid

    #73276

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB2909(XII-5 MarkerFree)

    Plasmid

    #73281

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX304_zeo_empty

    Plasmid

    #160093

    Purpose

    Control lentiviral vector for mammalian gene expression

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. JN302: pMVP/SB/Puro-DEST

    Plasmid

    #121865

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Puro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-GG-hUBC-EBFP2

    Plasmid

    #212314

    Purpose

    Lentiviral backbone with EBFP2 reporter

    Depositor

    Andrew Wilson

    Article

    Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-CAG-dDIO-MCS--4x6T

    Plasmid

    #196422

    Purpose

    Empty vector for astrocyte-selective Dre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Type

    Empty backbone

    Use

    AAV; Dre/rox; astrocyte-selective

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZDonor-Nm-Cas9-gRNA-hU6

    Plasmid

    #61366

    Purpose

    empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNA

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAV10.KH

    Plasmid

    #63208

    Purpose

    pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. LC101: pMVP/PB/Neo-DEST

    Plasmid

    #121875

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ Neo selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2+/N-Halo

    Plasmid

    #184417

    Purpose

    vector backbone to clone insert downstream of Halo tag (Halo tag codon optimized for Xenopus), for mRNA synthesis

    Depositor

    Ann Miller

    Article

    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

    Type

    Empty backbone

    Use

    For mrna synthesis

    Tags

    Halo-tag

    Expression

    Mutation

    Codon optimized for expression in Xenopus laevis …

    Promoter

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLCHKO

    Plasmid

    #155048

    Purpose

    Lentiviral backbone for cloning and expressing U6 driven hybrid guide (hg)RNAs with BfuAI cloning sites and puromycin selection.

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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