We narrowed to 63,667 results for: cloning
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Plasmid#137880PurposeDonor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFPDepositorInsertTRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
UseAAVExpressionMammalianAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TEVSH
Plasmid#125194PurposeHis-tagged TEV protease with solubility increasing mutationsDepositorInsertTEV_SH
TagsV5 His-tagExpressionBacterialPromoterT7lacAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKC-4.06
Plasmid#112084PurposeNMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3XFLAG firefly luciferase-beta-globin 39PTC (HBB Mouse, Human, Photinus pyralis)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-tdMCP-YFP
Plasmid#99151PurposeExpresses dimeric MS2-coat binding protein tagged with YFP, 2nd generation lentiviral vectorDepositorInsertDimeric MS2-coat binding protein
UseLentiviralTagseYFPExpressionMammalianPromoterSFFVAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LVXN-Neo-NSD2
Plasmid#86010Purposeexpress wild-type NSD2 in mammalian cellsDepositorInsertNSD2 (NSD2 Human)
UseLentiviralTagsFlag tag D Y K D D D D KExpressionBacterialMutationK1002R (please see depositor comments below)PromoterCMVAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLgw V5-EcoDam
Plasmid#59210PurposeMammalian DamID control lentiviral vector for expression of V5-Dam onlyDepositorInsertEcoDam
UseLentiviral; DamidTagsV5ExpressionMammalianPromoterHeat Shock Minimal Promoter and heat shock minima…Available sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-GCN5
Plasmid#65386PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGCN5 (KAT2A Human)
TagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-ERK2
Plasmid#37145DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertERK2 (MAPK1 Rat)
TagsEGFPExpressionMammalianMutationaa1-6 deleted as a result of cloning strategyPromoterCMVAvailable sinceNov. 14, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag-HA-USP43
Plasmid#22583DepositorPublicationInsertUSP43 (USP43 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationnot FL, missing first 108 aaAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP29
Plasmid#22599DepositorPublicationAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAd 2B Ngn3-I-nGFP
Plasmid#19410DepositorAvailable sinceNov. 10, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry
Plasmid#188779PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsZim3 KRAB-NLS fusionPromoterSFFVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI
Plasmid#189925PurposeAAV genome encoding SauriABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSauriABE8e
UseAAVMutationSauriCas9 D15APromoterEFSAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUCmini-iCAP-AAV.MaCPNS2
Plasmid#185137Purposenon-standard AAV2 rep-AAV-MaCPNS2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-MaCPNS2 VP1 gene
UseAAVExpressionMammalianPromoterp41Available sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSecUAG-Evol2
Plasmid#163148PurposeExpresses machinery necessary for selenocysteine incorporation at UAG codonsDepositorInsertsallo-tRNA UTu2D
thioredoxin
Selenophosphate synthase
Selenocysteine synthase
Cysteine desulfurase
ExpressionBacterialMutationCysteine 32 switched to Selenocysteine (UAG), Cys…PromoterEM7, araBAD, native As SelD promoter, and native …Available sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223 SARS-CoV-2 ORF8
Plasmid#141278PurposeGateway-compatible Entry vector, with insert of ORF8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertORF8 (ORF8 SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pT077
Plasmid#137879PurposeDonor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFPDepositorInsertTRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
UseAAVExpressionMammalianAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
R4pGWB601_UBQ10p-Turbo-YFP-NLS
Plasmid#127368PurposeBinary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plantsDepositorInsertTurboID (BirA mutant)
TagsGS linker, NLS, V5, and YFPExpressionPlantMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterArabidopsis UBQ10 promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac2.0
Plasmid#119872PurposeCumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR
sfGFP
UseSynthetic BiologyExpressionBacterialPromoterPveg promoter with CuO sequence and PxylR promoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZS157 CRISPEY RT/Cas9
Plasmid#114454PurposeYeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expressionDepositorInsertSpCas9, Ec86-RT
UseCRISPRTagsSV40-NLSExpressionYeastMutationS. cerevisiae codon optimizedPromoterGAL1-GAL10Available sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by