We narrowed to 62,913 results for: cloning

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lenti-EF1a-dCas9-VP64-Puro

Plasmid

#99371

Purpose

3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

Depositor

Kristen Brennand

Publication

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

dCas9-VP64-T2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pTubb3-MC

Plasmid

#87112

Purpose

minicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITI

Depositor

Juan Belmonte

Publication

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

GFP

Use

CRISPR

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pRetroX-Tight-MCS_PGK-OgNLuc

Plasmid

#70186

Purpose

Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

Depositor

Antonio Amelio

Publication

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

PGK

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDEST47-ARL8B-GFP

Plasmid

#67404

Purpose

Mammalian expression of hArl8b with C-term GFP tag

Depositor

Richard Kahn

Publication

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8B (ARL8B Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDDGFP_LEU2D

Plasmid

#58352

Purpose

Used for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiae

Depositor

Simon Newstead

Publication

Parker et al Protein Sci. 2014 Jun 20. doi: 10.1002/p

Type

Empty backbone

Tags

GFP-His

Expression

Yeast

Promoter

GAL1

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pENTR-LUC (w158-1)

Plasmid

#17473

Purpose

Gateway entry vector, Firefly Luciferase

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Gateway Cloning

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pENTR/pSM2(CMV) GFP (w513-1)

Plasmid

#19170

Purpose

Gateway entry vector for miRNA expression under CMV promoter and GFP reporter

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and RNAi

Tags

GFP

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry

Plasmid

#188779

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

Zim3 KRAB-NLS fusion

Promoter

SFFV

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307-DPP4-puro

Plasmid

#158451

Purpose

Constitutive lentiviral expression of human DPP4.

Depositor

Alejandro Chavez, Sho Iketani

Insert

DPP4 (DPP4 Human)

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCT5-bac2.0

Plasmid

#119872

Purpose

Cumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

Depositor

Claudia Schmidt-dannert

Publication

Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

Inserts

CymR
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pveg promoter with CuO sequence and PxylR promoter

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCIpA102-G-HLA-A2_GFP

Plasmid

#85162

Purpose

for mRNA expression of HLA.A2-EGFP

Depositor

Tone Fredsvik Gregers, Sébastien Walchli

Publication

Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

Insert

HLA.A2 (HLA-A Human)

Use

Mrna expression

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pT2/LTR7-GFP

Plasmid

#62541

Purpose

LTR7-driving reporter

Depositor

Zsuzsanna Izsvak

Publication

Wang et al Nature. 2014 Dec 18;516(7531):405-9. doi

Insert

LTR7-GFP

Use

Sleeping beauty transposon

Expression

Mammalian

Promoter

LTR7

Available since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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GFP-ASH1L

Plasmid

#65369

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Publication

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

ASH1L (ASH1L Human)

Tags

EmGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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human Alpha-synuclein

Plasmid

#51437

Purpose

human codon optimized Alpha-synuclein

Depositor

Michael J Fox Foundation MJFF

Insert

Alpha-synuclein (SNCA Human)

Use

Cloning

Available since

March 31, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pRS316-RGR-GFP

Plasmid

#51056

Purpose

Express self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.

Depositor

Yunde Zhao

Publication

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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tetO-NKX2.5

Plasmid

#46029

Depositor

John Gearhart

Publication

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

NKX2.5 (NKX2-5 Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Promoter

tetO

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p6600 MSCV-IP N-HAonly JUN

Plasmid

#34898

Depositor

Peter Howley

Publication

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

JUN (JUN Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pDONR223-NTRK1

Plasmid

#23891

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NTRK1 (NTRK1 Human)

Use

Gateway Cloning

Mutation

V263L relative to NM_001012331

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pENTRY-GFP

Plasmid

#15301

Depositor

William Hahn

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

hrGFP

Expression

Mammalian

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles
Cytoplasmic PancACe

Plasmid

#215708

Purpose

Expresses acetyl-CoA biosensor in the cytoplasm

Depositor

Katharine Diehl

Publication

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and cytoplasmic localization sequence

Expression

Mammalian

Promoter

CMV

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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