We narrowed to 7,126 results for: ef1a

1. pLV Cenpv-V5

   Plasmid

   #175125

   Purpose

   Lentiviral expression of mouse Cenpv-V5

   Depositor

   Larry Gerace

   Insert

   Cenpv (Cenpv Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSBbi-Pur-AktAR2-D

   Plasmid

   #125196

   Purpose

   SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of AktAR2 variant of FRET-based AKT activity reporter

   Depositor

   Eric Davis

   Insert

   AktAR2-D
   

   Use

   Transposon

   Tags

   6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…

   Expression

   Mammalian

   Mutation

   FoxO1 Thr-24 changed to Val rendering this inacti…

   Promoter

   EF1a/RPBSA

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. p11L2w

   Plasmid

   #37331

   Depositor

   John Schiller

   Article

   Kieback et al Virology. 2006 Feb 5;345(1):199-208. Epu

   Insert

   HPV11 L2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. iF53 PB-Zim3-dCas9-mScarlet-Hygro

   Plasmid

   #204726

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Zim3-dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Aiolos AA130-189 in Artichoke

   Plasmid

   #74451

   Purpose

   Monitor post-translational degradation of Aiolos AA130-189 (Lentiviral, SFFV.AiolosAA130-189-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

   Depositor

   Benjamin Ebert

   Insert

   Aiolos (IKZF3) AA130-189 (IKZF3 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   SFFV and EF1a

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. iF58 PB-Zim3-dCas9-mScarlet-Puro

   Plasmid

   #204728

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Zim3-dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKAM-E-Flag-GFP

   Plasmid

   #101866

   Purpose

   Lentiviral Evi-1-Flag with GFP marker

   Depositor

   Archibald Perkins

   Insert

   Evi-1 (Mecom Mouse)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV Cbx1-V5

   Plasmid

   #175106

   Purpose

   Lentiviral expression of mouse Cbx1-V5

   Depositor

   Larry Gerace

   Insert

   Cbx1 (Cbx1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. iD20 LoxStopLox-Zim3-dCas9-mApple-hygro

   Plasmid

   #204720

   Purpose

   Cre-inducible CRISPRi by Zim3-dCas9

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Zim3-dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV Lnpk-V5

    Plasmid

    #175121

    Purpose

    Lentiviral expression of mouse Lnpk-V5

    Depositor

    Larry Gerace

    Insert

    Lnpk (Lnpk Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. ELL1 CtoA

    Plasmid

    #127279

    Purpose

    Testing if ELL1 C to A mutation knock out the action on Myc as it does in human

    Depositor

    Christine Milcarek

    Insert

    ELL1 (NM_007924 plus CEYLHNK to AEYLHNK) (ELL Mouse)
    

    Use

    Tags

    Twin strep-tag

    Expression

    Mammalian

    Mutation

    CEYLHNK to AEYLHNK by Q5 mutagenesis

    Promoter

    EF1a

    Available since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

    Plasmid

    #193139

    Purpose

    Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

    Promoter

    EF1a

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKAM-E-Flag-BFP

    Plasmid

    #101867

    Purpose

    Lentiviral Evi-1-Flag with dual BFP and puro markers

    Depositor

    Archibald Perkins

    Insert

    Evi-1 (Mecom Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKAM-ME-Flag-BFP

    Plasmid

    #101868

    Purpose

    Lentiviral Mds1-Evi-1-Flag with dual BFP and puro markers

    Depositor

    Archibald Perkins

    Insert

    Mds1-Evi-1 (Mecom Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. MLCK_pLVX-EF1-Cry2-mCherry

    Plasmid

    #64369

    Purpose

    lentiviral expression of Myosin Light Chain Kinase and Cry2-mCherry

    Depositor

    Matthieu Piel

    Insert

    Myosin Light Chain Kinase (MYLK Human)
    

    Use

    Lentiviral

    Tags

    Cry2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

16. PB-TO-MYOD1-shOct4

    Plasmid

    #182309

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expression

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    MYOD1-shOct4 (MYOD1 Human)
    puro-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    shOct4

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJW1511

    Plasmid

    #62365

    Purpose

    pEF1a-puro-T2A-ATHmmuRPL10A

    Depositor

    Jonathan Weissman

    Article

    Jan et al Science. 2014 Nov 7;346(6210):1257521. d

    Insert

    Puro-T2A-AVITEVHAmmuRPL10A
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. iE61 PB-Zim3-XTEN-dCas9-mScarlet-puro-BFP

    Plasmid

    #204722

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-hACE2-cMYC-Flag

    Plasmid

    #158957

    Purpose

    Used for expression of human ACE2 with AAV packaging

    Depositor

    Akiko Iwasaki

    Insert

    Human ACE2 (ACE2 Human)
    

    Use

    AAV

    Tags

    Flag and MYC

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiGuide-Puro PRMT5-CRISPRi

    Plasmid

    #164637

    Purpose

    lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2

    Depositor

    Günter Schneider

    Insert

    target guide sequence PRMT5 inhibition (PRMT5 Human)
    

    Use

    CRISPR and Lentiviral; Interference

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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