We narrowed to 7,566 results for: ef1a;

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pGIPZ EF1a spnull-3xFLAG-DR18 IRES mNeonGreen-P2A-Puro

Plasmid

#248037

Purpose

Constitutively expresses 3xFLAG tagged DR18, mNeonGreen and a puromycin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Insert

3xFLAG-tagged DR18

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

human EF1a

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pGIPZ EF1a ACCx2 uORF eIL12 IRES mNeonGreen-P2A-Puro

Plasmid

#248035

Purpose

Constitutively expresses an engineered version of IL12, mNeonGreen and a puromycin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Insert

Engineered IL12

Use

Lentiviral

Expression

Mammalian

Promoter

human EF1a

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pGIPZ EF1a spnull-3xFLAG-Neo2/15 IRES mNeonGreen-P2A-Puro

Plasmid

#248033

Purpose

Constitutively expresses 3xFLAG tagged Neoleukin 2/15, mNeonGreen and a puromycin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Insert

3xFLAG-tagged Neoleukin-2/15

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

human EF1a

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2742 - hPGK-EGFP-SUGT1(C-term)-IRES2-mCherry-EF1A-Puro

Plasmid

#250273

Purpose

Ratiometric protein stability reporter for measuring C-terminal degron activity.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

EGFP-SUGT1(C-term)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2744 - hPGK-EGFP-GOPC(C-term)-IRES2-mCherry-EF1A-Puro

Plasmid

#250275

Purpose

Ratiometric protein stability reporter for measuring C-terminal degron activity.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

EGFP-GOPC(C-term)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2741 - hPGK-EGFP-CycD1(C-term)-IRES2-mCherry-EF1A-Puro

Plasmid

#250272

Purpose

Ratiometric protein stability reporter for measuring C-terminal degron activity.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

EGFP-CycD1(C-term)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pFB-EF1a-DIO-Rpl22l1-myc-Flag-2A-tdTomato-WPRE-hGHpA

Plasmid

#246243

Purpose

Cre dependent flag-tagged Rpl22l1.

Depositor

D. James Surmeier

Article

Tanimura et al Neuron. 2019 Feb 6;101(3):444-458.e6. do

Insert

Rpl22l1 (Rpl22l1 Mouse)

Use

AAV

Tags

Myc-FLAG-T2A-tdTomato

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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XZ066-(PB)EF1a-LIDARST-CCR6V2-IRESv0-T2A-PuroR-P2A-BFP

Plasmid

#233418

Purpose

PiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

June 13, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-[EF1a-mRuby2-bGH]-UbC-PuroR-T2A-mGL-SV40pA

Plasmid

#235325

Purpose

PiggyBac integration vector for ComMAND base gene regulating mRuby2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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XZ079 (PB)EF1a-CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Plasmid

#233435

Purpose

PiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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LSM042 (PB)EF1a-AVPR-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Plasmid

#233426

Purpose

PiggyBac donor plasmid AVPR2-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

AVPR-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-FLAG-mouse Beta 2 Adrenergic Receptor (Adrb2)

Plasmid

#233041

Purpose

To Express a CRE dependent Flag tagged Mouse Beta 2 Adrenergic Receptor (Adrb2) from an EF1a promoter

Depositor

Jonathan Ploski

Insert

mouse Beta 2 Adrenergic Receptor (Adrb2 Mouse)

Use

AAV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR

Plasmid

#218916

Purpose

Lentiviral construct expressing msfGFP and nuclear mCherry proteins.

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

EF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR

Use

Lentiviral

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(PAMmut)

Plasmid

#176139

Purpose

EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

XRCC1-T2A-PolB (XRCC1, POLB Human)

Use

Lentiviral

Tags

EGFP and MYC

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pNP2922 [pNP2321 - Cloning backbone, bRNA Landing Pad, with Ef1a-ISCro4 Recombinase]

Plasmid

#247255

Purpose

Cloning backbone for cloning bridge RNAs for ISCro4

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

Plasmid

#183410

Purpose

piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells

Depositor

Azim Surani

Article

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Insert

KRAB-dCas9-ecDHFR

Use

CRISPR and Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE3G

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pGEP116[EF1a-mNeonGreen]-pAAVS1-SA-Neo-TRE3Gs-NGN2-2A-Zeo-CAG-TETON3G

Plasmid

#243805

Purpose

Integrates dox-inducible hNGN2 into the human AASV1 safe harbor locus for directed differentiation of neural cells from stem cells

Depositor

Michael Wells

Insert

NGN2 (NEUROG2 Human)

Use

TALEN

Promoter

TRE3GS

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

Plasmid

#180280

Purpose

The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI sgRNA (backbone) and BFP

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)

Plasmid

#214885

Purpose

Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

Plasmid

#105842

Purpose

Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment

Depositor

Michael Ward

Article

Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

Inserts

Neurogenin 2 (NEUROG2 Human)
ISL LIM Homeobox 1 (ISL1 Human)
LIM Homeobox 3 (LHX3 Human)
Optimized mApple
rtTA3G
SBP Delta-LNGFR

Use

CRISPR and TALEN

Promoter

CAG, EF-1alpha, and TRE3G

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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