We narrowed to 5,944 results for: pCas

Showing: 1961 - 1980 of 5944 results

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pCAGGS puro HA-hUSP27X F313V

Plasmid

#225722

Purpose

Transfection of USP27X (F313V) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X F313V (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

F313V

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X K181E

Plasmid

#225721

Purpose

Transfection of USP27X (K181E) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X K181E (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

K181E

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X Y144C

Plasmid

#225720

Purpose

Transfection of USP27X (Y144C) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X Y144C (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

Y144C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X G76S

Plasmid

#225719

Purpose

Transfection of USP27X (G76S) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X G76S (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

G76S

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-PCaspase

Plasmid

#214034

Purpose

Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PCaspase

Tags

Strep-tag II

Expression

Bacterial

Mutation

WT

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-dPCaspase

Plasmid

#214035

Purpose

Bacterial expression plasmid for strep-tagged catalytically dead PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PCaspase

Tags

Strep-tag II

Expression

Bacterial

Mutation

H79A, C146A

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-PspCas13b_gRNA[ccdbCam]-2xPP7

Plasmid

#196846

Purpose

backbone for gRNA cloning of dpspCas13b tagged by 2xPP7

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Type

Empty backbone

Use

CRISPR

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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gzk328-dpspCas13b_gRNA[ccdbCam]-10xPBSc_Loop

Plasmid

#196844

Purpose

backbone for gRNA cloning of dpspCas13b tagged by 10xPBSc_Loop

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Type

Empty backbone

Use

CRISPR

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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gzk326-dpspCas13b_gRNA[ccdbCam]-5xPBSc_Loop

Plasmid

#196845

Purpose

backbone for gRNA cloning of dpspCas13b tagged by 5xPBSc_Loop

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Type

Empty backbone

Use

CRISPR

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-AnkG ZU5in-3xHA

Plasmid

#213730

Purpose

Mammalian cell expression vector for overexpression of AnkG ZU5in isoform in motor neurons.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

AnkG ZU5in (Ank3 Mouse)

Expression

Mammalian

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9HF1-P2A-puro

Plasmid

#214357

Purpose

Expresses SpCas9HF1 and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SpCas9HF1 and puromycin resistance genes

Expression

Mammalian

Mutation

N497A/R661A/Q695A/Q926A

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-AnkB ZU5ex-3xHA

Plasmid

#213732

Purpose

Mammalian cell expression vector for overexpression of AnkB ZU5ex isoform in motor neurons.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

AnkB ZU5ex (Ank2 Mouse)

Expression

Mammalian

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-AnkG ZU5ex-3xHA

Plasmid

#213731

Purpose

Mammalian cell expression vector for overexpression of AnkG ZU5ex isoform in motor neurons.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

AnkG ZU5ex (Ank3 Mouse)

Expression

Mammalian

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCaUra3-CUbo(K48R)-yeGFP

Plasmid

#212772

Purpose

C-terminal PCR-based tagging with CUbo(K48R)-yeGFP in budding yeast with CaUra3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCaUra3-CUbo(K63R)-yeGFP

Plasmid

#212773

Purpose

C-terminal PCR-based tagging with CUbo(K63R)-yeGFP in budding yeast with CaUra3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro

Plasmid

#211821

Purpose

PiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishment

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

SpCas9-ABE8e, tracrRNA and Puromycin resistance gene

Expression

Mammalian

Promoter

chicken β-actin promoter, U6 promoter and hPGK pr…

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGIG-FLAG-SHTN1LRRR>GGG

Plasmid

#209570

Purpose

Mammalian expression of Shtn1 long isoform with RG mutation

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform with RG mutation (4930506M07Rik Mouse)

Expression

Mammalian

Mutation

RRR to GGG

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 BBS exon37a pCAGGS

Plasmid

#206086

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and alternative exon 37a

Depositor

Annette Dolphin

Article

Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV/B-actin

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA EIVA (E1658A) pCAGGS

Plasmid

#206082

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain IV which reduces trafficking to the cell surface

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

E1658A

Promoter

CMV/B-actin

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA EIA (E314A) pCAGGS

Plasmid

#206081

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain I which reduces trafficking to the cell surface

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

E314A

Promoter

CMV/B-actin

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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