We narrowed to 8,683 results for: sgRNA

1. B-HypaR-SpCas9

   Plasmid

   #126764

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Hypa2SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A; N692A; M694A; Q695A; H698A; amino acids 10…

   Promoter

   CBh

   Available Since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-sgCtrl_EF1a-Puro-T2A-BFP

   Plasmid

   #195500

   Purpose

   Lentiviral BFP expression vector bearing a sgRNA targeting a randomized human TSS sequence

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP, Puro, and T2A

   Expression

   Mammalian

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-sgT-REX17_EF1a-Puro-T2A-BFP

   Plasmid

   #195501

   Purpose

   Lentiviral BFP expression vector bearing a sgRNA targeting the promoter of human T-REX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP, Puro, and T2A

   Expression

   Mammalian

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJW1884

   Plasmid

   #154332

   Purpose

   sgRNA Target Vector for CRISPR

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   cxTi10882 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR
   

   Use

   CRISPR

   Expression

   Worm

   Mutation

   F+E mutations in sgRNA

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTargetF

   Plasmid

   #62226

   Purpose

   Constitutive expression of sgRNA without donor editing template DNA

   Depositor

   Sheng Yang

   Article

   Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

   Insert

   sgRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   pij23119

   Available Since

   March 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-TO-dCas9

   Plasmid

   #75381

   Purpose

   dCas9

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   Sp dCas9
   

   Use

   Lentiviral

   Tags

   no

   Expression

   Mammalian

   Promoter

   CMV-TO

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. U6>Control(F+E)

   Plasmid

   #60006

   Purpose

   U6 promoter driving control (F+E) sgRNA

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Insert

   Control (F+E) sgRNA
   

   Use

   CRISPR

   Promoter

   Ciinte.U6

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pV1093

   Plasmid

   #111428

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1

   Depositor

   Gerald Fink

   Article

   Vyas et al Sci Adv. 2015;1(3):e1500248.

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCFD6

   Plasmid

   #73915

   Purpose

   tissue-specific multiplex gRNA plasmid

   Depositor

   Simon Bullock

   Article

   Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

   Insert

   :tRNA:gRNA:tRNA:gRNA:tRNA
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   UAS-DSCP

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3s-Cas9-HN

    Plasmid

    #104171

    Purpose

    Cas9-WT mammalian expression

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

    Insert

    Cas9-WT
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTol2_NBT:Cas9green; erbb2_gRNA171

    Plasmid

    #199337

    Purpose

    pDEL134; transgenic construct to express cell-specific Cas9 in neurons; ubiquitous erbb2 sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    NBT
    Cas9
    erbb2 sgRNA
    

    Use

    Tol2 destination vector

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUCSh5vio-2300

    Plasmid

    #170987

    Purpose

    Encodes sgRNA target 2300 and Gentamicin resistance - violacein pathway transposon.

    Depositor

    Christopher Reisch

    Article

    Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

    Insert

    Sh-CAST sgRNA 2300 violacein
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    placI

    Available Since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAL-rfp_T

    Plasmid

    #74075

    Purpose

    pAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    sgRNA rfp (T)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    ptac

    Available Since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTO136_pADH1-tRNA-Ala-gRNA-tracrRNA-HDV-tAgTEF2

    Plasmid

    #171104

    Purpose

    Expresses sgRNA (ENO1 gRNA) in Candida auris.

    Depositor

    Teresa O'Meara

    Article

    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

    Insert

    sgRNA
    

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330_SYCP3_nterm

    Plasmid

    #222524

    Purpose

    Cas9/sgRNA plasmid for targeting SYCP3

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    Cas9, SYCP3 sgRNA (SYCP3 Human, Synthetic)
    

    Use

    CRISPR

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJMP2832

    Plasmid

    #160672

    Purpose

    Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

    Depositor

    Jason Peters

    Article

    Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

    Insert

    gmc6 (gfp) sgRNA
    

    Expression

    Bacterial

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJMP2820

    Plasmid

    #160669

    Purpose

    Mobile CRISPRi sfGFP 'test', empty sgRNA

    Depositor

    Jason Peters

    Article

    Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

    Insert

    empty sgRNA (BsaI)
    

    Expression

    Bacterial

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. ttAtCas12a+int

    Plasmid

    #182384

    Purpose

    Gives very high editing efficiency in barley. Also works in Brassica oleracea

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al bioRxiv 2022.04.28.489853

    Insert

    LbCas12a coding sequence with D156R and Arabidopsis introns
    

    Use

    CRISPR

    Mutation

    D156R + 8 Arabidopsis introns added

    Promoter

    None

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. zCREST3:redCas9; control_gRNA

    Plasmid

    #199334

    Purpose

    pDEL157; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous control sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    zCREST3
    Cas9
    control sgRNA
    

    Use

    Tol2 destination vector

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJW1219

    Plasmid

    #61250

    Purpose

    CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    sgRNA(F+E)
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6 promoter

    Available Since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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