We narrowed to 23,181 results for: ARC;

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PB-U6insert-EF1puro

Plasmid

#104537

Purpose

A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

Depositor

George Church

Article

Kalhor et al Science. 2018 Aug 9. pii: science.aat980

Type

Empty backbone

Use

CRISPR, Mouse Targeting, and Synthetic Biology ; …

Expression

Mammalian

Promoter

U6

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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PX855

Plasmid

#62887

Purpose

N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa 2-535)

Use

CRISPR

Tags

FRB and NES PTK2

Expression

Mammalian

Mutation

Aspartic acide 10 to Alanine (D10A)

Promoter

CBh

Available Since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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pAC572

Plasmid

#127973

Purpose

AMA1 plasmid with pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)

Depositor

Uffe Mortensen

Article

Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

Insert

pyrG

Use

Fungal expression

Expression

Bacterial

Promoter

native promoter

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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p246 eSpCas9_2gRNAs_mH11

Plasmid

#164854

Purpose

gRNA vector for targeting mouse H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

Insert

gRNAs for targeting mouse H11 locus

Expression

Mammalian

Promoter

U6

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1

Plasmid

#221472

Purpose

Gateway cloning of NKX2-1 short isoform overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway Cloning

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMXLK_aFb

Plasmid

#114230

Purpose

POC12368, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type a-b

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Lifeact-Halo

Plasmid

#193328

Purpose

mammalian transient expression - expression of Lifeact-Halo

Depositor

Klaus Hahn

Article

Herron et al Nat Commun. 2022 Jul 27;13(1):4363. doi:

Insert

Lifeact

Expression

Mammalian

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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PX852

Plasmid

#62884

Purpose

C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa536-1368)

Use

CRISPR

Tags

FKBP12, NLS SV40, and NLS nucleoplasmin

Expression

Mammalian

Promoter

CBh

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pOGG039

Plasmid

#113996

Purpose

SC module. Terminator for T7RNAP

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Terminator for T7RNAP

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pGL1_37

Plasmid

#199044

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

RSF1010 ORI plus NGS barcode 14

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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