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Plenti-CMV-MCS-RFP-SV-puro

Plasmid

#109377

Purpose

This plasmid expresses dsRed2 and puromycin resistance

Depositor

Jonathan Garlick, Behzad Gerami-Naini

Type

Empty backbone

Use

Lentiviral

Tags

dsRed2

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-mEGFP-CaMKIIa (T286A)

Plasmid

#127390

Purpose

Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286A)

Depositor

Ryohei Yasuda

Article

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)

Tags

mEGFP

Expression

Mammalian

Mutation

changed Threonine 286 to Alanine

Promoter

pCAG

Available Since

Jan. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_mCherry_mito Grx1-roGFP2

Plasmid

#155044

Purpose

Fluorescent redox reporter

Depositor

Vanessa LaPointe

Insert

Glutaredoxin-1 (GLRX Human)

Use

Lentiviral

Tags

mitochondrial targeting sequence and roGFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-sumo-h-cGAS

Plasmid

#127161

Purpose

expression of human cGAS protein

Depositor

Thomas Tuschl

Article

Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

Insert

MB21D1 (CGAS Human)

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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ADORA1-DuET

Plasmid

#213173

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

ADORA1 (ADORA1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-EGFP-C1-centrin-1

Plasmid

#73331

Purpose

Lentiviral vector encoding centrin-1 with an N-terminal EGFP tag

Depositor

Manuel Thery

Insert

centrin-1 (CETN1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR wt

Plasmid

#214699

Purpose

Mammalian expression of human CALR wt

Depositor

Norio Komatsu

Article

Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

Insert

CALR wt (CALR Human)

Use

Retroviral

Promoter

MSCV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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RMCE K>R DNA-PKcs

Plasmid

#233917

Purpose

Low copy number provides expression of human DNA-PKcs with a single K>R substitution that ablates kinase activity.

Depositor

Katheryn Meek

Article

Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

Insert

human DNA-PKcs kinase inactive mutant K3753>R (PRKDC Human)

Expression

Mammalian

Mutation

K3753R

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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LRRK2-WT

Plasmid

#17609

Depositor

Ted Dawson

Article

West et al Proc Natl Acad Sci U S A. 2005 Nov 15;10

Insert

LRRK2 (LRRK2 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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TFORF2246

Plasmid

#141989

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TCF12 (TCF12 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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