8,139 results

1. p426-SNR52p-gRNA.CAN1.Y-SUP4t

   Plasmid

   #43803

   Purpose

   Encodes a gRNA that targets Can1.Y in yeast.

   Depositor

   George Church

   Article

   Dicarlo et al Nucleic Acids Res

   Insert

   CAN1.y gRNA (CAN1 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   SNR52

   Available Since

   March 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-HaloTag-KanMX6

   Plasmid

   #87029

   Purpose

   Tag S. pombe genes in C terminal with HaloTag

   Depositor

   Julien Berro

   Insert

   HaloTag
   

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYZ463

   Plasmid

   #187971

   Purpose

   TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

   Insert

   Cas9 (codon optimized for human)
   

   Use

   Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPL5071_TEF1*-Cre_URA3

   Plasmid

   #60930

   Purpose

   Constitutive expression vector for Cre-recombinase containing URA3 selection marker.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   Cre
   

   Tags

   Driven from a mutant TEF1 promoter with 0.16x act…

   Expression

   Yeast

   Promoter

   mutant TEF1

   Available Since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYZ960

   Plasmid

   #187972

   Purpose

   TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2µ-URA3

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

   Insert

   Cas9 (codon optimized for human)
   

   Use

   Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYZ462

   Plasmid

   #187970

   Purpose

   TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

   Insert

   Cas9 (codon optimized for human)
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYZ959

   Plasmid

   #192603

   Purpose

   TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with CEN-URA3

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

   Insert

   Cas9 (codon optimized for human)
   

   Use

   Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Promoter

   ScTEF1

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDZ645 pKAN 1x-mCherry-12xPP7 V4

   Plasmid

   #73173

   Purpose

   Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   1x-mCherry
   

   Expression

   Mammalian and Yeast

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNB655

   Plasmid

   #60142

   Purpose

   Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus
   

   Expression

   Yeast

   Mutation

   A4V

   Promoter

   MET17pr

   Available Since

   Feb. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG416-GAL-SUP35

    Plasmid

    #181705

    Purpose

    Galactose inducible expression of SUP35

    Depositor

    Alejandro Chavez

    Insert

    SUP35 (SUP35 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG416-GAL-RNQ1

    Plasmid

    #181706

    Purpose

    Galactose inducible expression of RNQ1

    Depositor

    Alejandro Chavez

    Insert

    RNQ1 (RNQ1 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGKB

    Plasmid

    #85077

    Purpose

    expression in Pichia pastoris

    Depositor

    Sheng Yang

    Article

    Yang et al Yeast. 2014 Apr;31(4):115-25.

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    GAP

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCYC1m_yeGFP

    Plasmid

    #64389

    Purpose

    encodes yeast enhanced GFP under control of minimal pCYC1 promoter

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    yeast enhanced GFP
    

    Expression

    Yeast

    Promoter

    pCYC1

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKanB

    Plasmid

    #223523

    Purpose

    Used for expression of intracellular recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

    Depositor

    Geoff Lin-Cereghino

    Article

    Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    Sept. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pND003

    Plasmid

    #200949

    Purpose

    Yeast surface display destination vector for Golden Gate cloning

    Depositor

    Timothy Whitehead

    Article

    Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

    Type

    Empty backbone

    Use

    Yeast surface display

    Tags

    AGA2, HA, and Myc

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSC-humanADAR1

    Plasmid

    #198235

    Purpose

    Expresses human ADAR1 in yeast

    Depositor

    Brenda Bass

    Article

    Eggington et al Nat Commun. 2011;2:319. doi: 10.1038/nco

    Insert

    human ADAR1 (ADAR Human)
    

    Tags

    His10_TEV

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFA6a-link-ytdTomato-URA3

    Plasmid

    #168054

    Purpose

    Replaces Addgene plasmid 118461. Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ytdTomato
    

    Tags

    ytdTomato

    Expression

    Yeast

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVT36b

    Plasmid

    #184437

    Purpose

    CRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1’-tRNA_Leu promoter, and sgRNA scaffold

    Depositor

    Huimin Zhao

    Article

    Tran et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. YEp181-CUP1-His-Ubi

    Plasmid

    #99539

    Purpose

    Yeast episomal vector for expression of His-tagged ubiquitin; LEU2 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    UBI3(1-76) (RPL40A Budding Yeast)
    

    Tags

    7xHis

    Expression

    Yeast

    Promoter

    CUP1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. FRP2371

    Plasmid

    #127577

    Purpose

    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, His3MX selection marker

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

    Insert

    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    

    Use

    Synthetic Biology; Shuttle vector

    Expression

    Yeast

    Available Since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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