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Addgene
Showing: 181 - 200 of 2059 results

  3. G protein alpha-s(alpha3beta5/G226A/A366S)

    Plasmid
    #55797
    Purpose
    This highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.
    Depositor
    Catherine Berlot
    Article
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Insert
    alpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
    Use
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T, G226A, A366S i…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    55797-plasmid-map-sequence-id-112181

    Enlarge

  4. G protein alpha-s(alpha3beta5/G226A)

    Plasmid
    #55798
    Purpose
    In addition to the mutations in alpha-s(alpha3beta5), this dominant negative G protein alpha-s mutant contains the G226A mutation, which impairs activating conformation changes in Switch II.
    Depositor
    Catherine Berlot
    Article
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Insert
    alpha-s (alpha3beta5/G226A) (Gnas Rat)
    Use
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T, G226A in alpha…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    55798-plasmid-map-sequence-id-112177

    Enlarge

  5. G protein alpha-s(alpha3beta5)

    Plasmid
    #55799
    Purpose
    This G protein alpha-s mutant exhibits increased affinity for and decreased ability to be activated by Gs-protein-coupled receptors as well as decreased ability to activate adenylyl cyclase.
    Depositor
    Catherine Berlot
    Article
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Insert
    G protein alpha-s with alpha-i2 substitutions in alpha3/beta5 loop (Gnas Rat)
    Use
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T in alpha-s sequ…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    55799-plasmid-map-sequence-id-119340

    Enlarge

  6. DsRed2-Cx43-7

    Plasmid
    #55834
    Purpose
    Localization: Gap Junctions, Excitation: 558, Emission: 583
    Depositor
    Michael Davidson
    Insert
    Cx43
    Use
    Tags
    DsRed2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. mRuby-CEBPA237-C-4

    Plasmid
    #55851
    Purpose
    Localization: Nucleus, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Insert
    CEBPA
    Use
    Tags
    mRuby
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. mRuby-Cx26-7

    Plasmid
    #55854
    Purpose
    Localization: Gap Junctions, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Insert
    Cx26
    Use
    Tags
    mRuby
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    55854-plasmid-map-sequence-id-221616

    Enlarge

  9. mRuby-Cx43-7

    Plasmid
    #55856
    Purpose
    Localization: Gap Junctions, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Insert
    Cx43
    Use
    Tags
    mRuby
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    55856-plasmid-map-sequence-id-201674

    Enlarge

  10. mRuby-Lysosomes-20

    Plasmid
    #55872
    Purpose
    Localization: Lysosome Membrane, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Insert
    LAMP1
    Use
    Tags
    mRuby
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. mRFP1-Cx43-7

    Plasmid
    #55919
    Purpose
    Localization: Gap Junctions, Excitation: 584, Emission: 607
    Depositor
    Michael Davidson
    Insert
    Cx43
    Use
    Tags
    mRFP1
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  14. mStrawberry-Cx26-7

    Plasmid
    #55936
    Purpose
    Localization: Gap Junctions, Excitation: 574, Emission: 596
    Depositor
    Michael Davidson
    Insert
    Cx26
    Use
    Tags
    mStrawberry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  15. mStrawberry-Cx43-7

    Plasmid
    #55937
    Purpose
    Localization: Gap Junctions, Excitation: 574, Emission: 596
    Depositor
    Michael Davidson
    Insert
    Cx43
    Use
    Tags
    mStrawberry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  16. mPlum-CathespinB-6

    Plasmid
    #55955
    Purpose
    Localization: Lysosomes, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    CathespinB (Ctsb Rat)
    Use
    Tags
    mPlum
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    55955-plasmid-map-sequence-id-173178

    Enlarge

  17. mPlum-Cx26-7

    Plasmid
    #55959
    Purpose
    Localization: Gap Junctions, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    Cx26 (Gjb2 Rat)
    Use
    Tags
    mPlum
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  18. mPlum-Cx43-7

    Plasmid
    #55961
    Purpose
    Localization: Gap Junctions, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    Cx43 (Gja1 Rat)
    Use
    Tags
    mPlum
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    55961-plasmid-map-sequence-id-335175

    Enlarge

  19. mPlum-Lysosomes-20

    Plasmid
    #55982
    Purpose
    Localization: Lysosome Membrane, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    LAMP1 (Lamp1 Rat)
    Use
    Tags
    mPlum
    Expression
    Mammalian
    Mutation
    D50E
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    55982-plasmid-map-sequence-id-147991

    Enlarge

Showing: 181 - 200 of 2059 results