We narrowed to 438 results for: 1864

1. scramble shRNA

   Plasmid

   #1864

   Purpose

   3rd gen lentiviral negative control vector containing scrambled shRNA

   Depositor

   David Sabatini

   Article

   Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

   Insert

   scramble
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   April 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pDGB3omega1_KanR_BastaR

   Plasmid

   #186426

   Purpose

   optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)

   Depositor

   Lukas Fischer

   Article

   Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

   Inserts

   KanR
   BastaR
   

   Use

   Synthetic Biology; Binary vector for escherichia …

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   CaMV 35S and Pnos

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPR v2 sgKEAP1-2

   Plasmid

   #186459

   Purpose

   knock out KEAP1 in mammalian cells

   Depositor

   Boyi Gan

   Article

   Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

   Insert

   Keap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCambia-PUP-IT

   Plasmid

   #186478

   Purpose

   Expresses POI-PUP-IT fusion in Plants

   Depositor

   Jen Sheen

   Article

   Ye et al Nature. 2022 Sep 14. pii: 10.1038/s41586

   Insert

   FLAG-Pup(E)
   

   Tags

   Triple Flag Tag

   Expression

   Plant

   Promoter

   35S

   Available Since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPLV_C1

   Plasmid

   #186415

   Purpose

   Endogenous murine RNase inhibitor production for cell-free lysate

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Sci Rep. 2022 Aug 12;12(1):13785. doi: 1

   Insert

   Murine RNase Inhibitor
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. 2Bc-T MBP_BoMoC(ed)_6xH

   Plasmid

   #186461

   Purpose

   Recombinant B. mori truncated R2 RT expression

   Depositor

   Kathleen Collins

   Article

   Upton et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

   Insert

   B. mori truncated R2 RT
   

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQCXIP mScarlet-MOSPD2 WT

   Plasmid

   #186472

   Purpose

   Expression of human MOSPD2 fused to mScarlet in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
   

   Use

   Retroviral

   Tags

   mScarlet

   Expression

   Mammalian

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. H3K9me3Probe_FullLength

   Plasmid

   #186463

   Purpose

   Expresses GFP tagged H3K9me3 recognition motif in mammalian cells.

   Depositor

   Chongli Yuan

   Article

   Mendonca et al Cytometry A. 2022 Apr;101(4):339-350. do

   Insert

   dimeric CDY1 (CDY1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-6P-acatenin

   Plasmid

   #186458

   Purpose

   Expresses human alpha-catenin with an N-terminal GST tag and a PRescission Protease cleavage site

   Depositor

   Tina Izard

   Article

   Rangarajan et al Commun Biol. 2023 Mar 16;6(1):276. doi:

   Insert

   CTNNA1 (CTNNA1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-EIN-FTO(32-505)

   Plasmid

   #186466

   Purpose

   Expresses human RNA demythelase protein FTO 32-505 amino acid sequence with N-terminal fusion with the N-domain of EI ( e.coli protein)

   Depositor

   Vincenzo Venditti

   Article

   Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

   Insert

   Fat mass obesity associated protein (FTO Human)
   

   Tags

   6 Histidine-EIN (N-terminal domain of E.coli Enzy…

   Expression

   Bacterial

   Mutation

   amino acid 32-505

   Promoter

   T7

   Available Since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2-mCherry-Cenexin

    Plasmid

    #186435

    Purpose

    Expresses mCherry Cenexin in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al Mol Biol Cell. 2022 Aug 1;33(9):br14. do

    Insert

    Cenexin (ODF2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. ZS006:eGFP-KRAS

    Plasmid

    #186433

    Purpose

    eGFP-KRAS

    Depositor

    Adam Cohen

    Article

    Shi et al Cell. 2018 Dec 13;175(7):1769-1779.e13.

    Insert

    eGFP-KRAS
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. dCas9 fused to scFv (anti-spike)

    Plasmid

    #186421

    Purpose

    Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2

    Depositor

    Stephen Elledge

    Article

    Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

    Insert

    dCas9-scFv fusion (anti-SARS-CoV-2 spike)
    

    Use

    CRISPR

    Tags

    6His

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15b MOSPD2 [282-490]

    Plasmid

    #186469

    Purpose

    Expression of human MOSPD2 (MSP domain from residue 282 to 490), fused to a 6HIS tag, in bacteria

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    MSP domain [282-490]

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. dCas9 fused to nanobody (anti-beta catenin)

    Plasmid

    #186420

    Purpose

    Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

    Depositor

    Stephen Elledge

    Article

    Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

    Insert

    dCas9-nanobody fusion (anti-beta catenin)
    

    Use

    CRISPR

    Tags

    6His

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pQCXIP GFP-MOSPD2 WT

    Plasmid

    #186467

    Purpose

    Expression of human MOSPD2 fused to EGFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDGB1alpha2_KanR_BastaR_Rb7_GFP

    Plasmid

    #186427

    Purpose

    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Inserts

    KanR
    BastaR
    Rb7
    eGFP
    

    Use

    Synthetic Biology; Binary vector for escherichia …

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    CaMV 35S and Pnos

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQCXIP EGFP-ER [TM(SAC1)]

    Plasmid

    #186475

    Purpose

    Expression of the Endoplasmic Reticulum marker GFP-ER [TM(SAC1)] in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    SAC1 (SACM1L Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C-terminal transmembrane region of the protein: 5…

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGB1alpha11_SulR

    Plasmid

    #186424

    Purpose

    transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter; suitable for quintuple assembly

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Insert

    SulR
    

    Use

    Synthetic Biology; Binary vector for escherichia …

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTarget

    Plasmid

    #186452

    Purpose

    used as source for acquisition of new spacers

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge