We narrowed to 15 results for: 62225

1. pCas

   Plasmid

   #62225

   Purpose

   Constitutive expression of cas9 and inducible expression of lambda RED and sgR

   Depositor

   Sheng Yang

   Article

   Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

   Has Service

   Cloning Grade DNA

   Insert

   cas9
   

   Use

   CRISPR; Lambda red recombinase

   Expression

   Bacterial

   Promoter

   native cas9 promoter

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pCasTet-λ

   Plasmid

   #128318

   Purpose

   Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

   Depositor

   Christopher Johnston

   Article

   Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

   Insert

   TetR and pTetR/TetO
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Mutation

   Constitutive expression of cas9 and anhydro-tetra…

   Promoter

   tet promoter

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLCNICK

   Plasmid

   #84653

   Purpose

   Genome editing for Lactobacillus casei Lc2W

   Depositor

   Sheng Yang

   Article

   Song et al Appl Environ Microbiol. 2017 Sep 1. pii:

   Inserts

   Cas9 nickase
   sgRNA
   homology arms of LC2W_2179
   

   Use

   CRISPR

   Mutation

   D10A

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHSP02

   Plasmid

   #117259

   Purpose

   Genome editing for Lactobacillus plantarum WCSF1

   Depositor

   Sheng Yang

   Article

   Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

   Insert

   Cas9, promotor P11-guide-RNA, homologous arms of Lp_0537
   

   Use

   Other

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHSB04X

   Plasmid

   #117258

   Purpose

   Genome editing for Lactobacillus brevis ATCC367

   Depositor

   Sheng Yang

   Article

   Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

   Insert

   Cas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
   

   Use

   Other

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR_EF1a-->HA-mtLaG2

   Plasmid

   #162225

   Purpose

   Lentiviral vector for the expression of HA GFP nanobody Lag2 in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2020 Oct 16;370(6514):327-331.

   Insert

   anti-GFP nanobody LaG2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-EGFP-SKL

   Plasmid

   #183727

   Purpose

   For exrpression of GFP targeted to peroxisomes

   Depositor

   Jeffrey Gerst

   Article

   Haimovich et al bioRxiv 2024.11.06.622258

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   SKL (peroxisomal targeting sequence 1)

   Promoter

   UbiC

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-RFP-SKL

   Plasmid

   #183726

   Purpose

   For expression of RFP targeted to peroxisomes

   Depositor

   Jeffrey Gerst

   Article

   Haimovich et al bioRxiv 2024.11.06.622258

   Insert

   mRFP1
   

   Use

   Lentiviral

   Tags

   SKL (peroxisomal targeting sequence 1)

   Promoter

   UbiC

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-mCardinal-SKL

   Plasmid

   #183728

   Purpose

   For exrpression of mCardinal targeted to peroxisomes

   Depositor

   Jeffrey Gerst

   Article

   Haimovich et al bioRxiv 2024.11.06.622258

   Insert

   mCardinal
   

   Use

   Lentiviral

   Tags

   SKL (peroxisomal targeting sequence 1)

   Promoter

   UbiC

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-mNeptune2-SKL

   Plasmid

   #183729

   Purpose

   For exrpression of mNeptune2 targeted to peroxisomes

   Depositor

   Jeffrey Gerst

   Article

   Haimovich et al bioRxiv 2024.11.06.622258

   Insert

   mNeptube2
   

   Use

   Lentiviral

   Tags

   SKL (peroxisomal targeting sequence 1)

   Promoter

   UbiC

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-PEX6-Tomato

    Plasmid

    #183730

    Purpose

    Expression of human PEX6 fused to Tomato, and PEX6 3'UTR

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Insert

    PEX6-Tomato-PEX63'UTR
    

    Use

    Lentiviral

    Promoter

    UbiC

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-7xHA-PEX6-Tomato

    Plasmid

    #183731

    Purpose

    Expression of human 7xHA- PEX6 fused to Tomato, and PEX6 3'UTR

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Insert

    PEX6-Tomato-PEX63'UTR
    

    Use

    Lentiviral

    Tags

    7xHA

    Promoter

    UbiC

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNX24-RFP-MS2SL

    Plasmid

    #227819

    Purpose

    Expresses RFP via CMV promoter. The mRNA is tagged with 24x MS2 stem-loops (version 3) at the 3'UTR. Also encodes Neo resistance gene

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Inserts

    3xHA-RFP-24xMS2SL
    Neo resistance
    

    Expression

    Mammalian

    Promoter

    CMV and SV40

    Available Since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-Cre-MS2_UbC-GFP (Cre_GFP)

    Plasmid

    #219536

    Purpose

    a. Expresses Cre recombinase tagged with 21x repeats of MS2 binding sequence under EF1A promotor and b. GFP reporter under UbC promotor

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Inserts

    Cre recombinase (cre )
    GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)

    Plasmid

    #219537

    Purpose

    a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Inserts

    Cre recombinase (cre )
    NLS-tdMCP-GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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