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Addgene
Showing: 81 - 100 of 129 results
  1. pGW-Dronpa-Cav1.2

    Plasmid
    #182333
    Purpose
    L-type voltage-gated calcium channel α1C subunit tagged with Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Insert
    Cav1.2-Dronpa (CACNA1C Rabbit)
    Use
    Tags
    Full-length rabbit Cav1.2.
    Expression
    Mammalian
    Mutation
    T1066Y, Q1070M
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  2. pH2B-6AA-ffDronpa

    Plasmid
    #214606
    Purpose
    Expresses reversibly switchable fluorescent protein ffDronpa targeted to the nucleus
    Depositor
    Insert
    ffDronpa-H2B
    Use
    Tags
    H2B targetting with a linker of 6AA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  3. pIA027

    Plasmid
    #200418
    Purpose
    amyE::(dronPA cm), for dronPA fusions. PALM-compatible integration vector for Bacillus subtilis. Includes dronPA photoswitchable FP with multi cloning sites (MCS) for fast and interchangeable cloning.
    Type
    Empty backbone
    Use
    Tags
    dronPA
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pIA035

    Plasmid
    #200426
    Purpose
    amyE::(Ppen-lacIΔ11-dronPA cm). PALM integration vector includes dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.
    Insert
    LacI
    Use
    Tags
    dronPA
    Expression
    Bacterial
    Mutation
    Promoter
    Ppen
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA3-Lyn-μDpTT

    Plasmid
    #182321
    Purpose
    Plasma membrane-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Insert
    Lyn-μDronpa-TagRFP-T
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  6. pcDNA3-DAKAP-μDpTT

    Plasmid
    #182324
    Purpose
    Mitochondria-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Insert
    DAKAP-μDronpa-TagRFP-T
    Use
    Tags
    N-terminal 30 amino acids from DAKAP1
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA3-CYP450-μDpTT

    Plasmid
    #182325
    Purpose
    Endoplasmic reticulum-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Insert
    DAKAP-μDronpa-TagRFP-T
    Use
    Tags
    N-terminal 27 amino acids from CYP450
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  8. pcDNA3-Lifeact-μDpTT

    Plasmid
    #182322
    Purpose
    Actin-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Insert
    Lifeact-μDronpa-TagRFP-T
    Use
    Tags
    N-terminal 17 amino acids from budding yeast Abp1…
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  9. pcDNA3-RIIα-μDpTT

    Plasmid
    #182327
    Purpose
    PKA RIIα subunit-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Insert
    RIIalpha-μDronpa-TagRFP-T (PRKAR2A Human)
    Use
    Tags
    Full-length human PKA type 2alpha regulatory subu…
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  10. pcDNA3-H2B-μDpTT

    Plasmid
    #182328
    Purpose
    Histone H2B-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Insert
    H2B-μDronpa-TagRFP-T (H2BC3 Human)
    Use
    Tags
    Full-length human histone H2B
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  11. pcDNA3-DrFLINC-AKAR-Kras

    Plasmid
    #182334
    Purpose
    Genetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.
    Depositor
    Insert
    DrFLINC-AKAR
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  12. pcDNA3-β2AR-μDpTT

    Plasmid
    #182326
    Purpose
    Beta-2 adrenergic receptor-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Insert
    beta2AR-μDronpa-TagRFP-T (ADRB2 Human)
    Use
    Tags
    Full-length human beta2 adrenergic receptor
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
Showing: 81 - 100 of 129 results