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gCH132 (crCD55-4_crB2M-1_crKIT-2_crKIT-3_crCD81-1)

Plasmid

#217341

Purpose

crRNA array targeting CD81, B2M, KIT, CD55

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Inserts

crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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gCH134 (crCD55-4_crB2M-1_crB2M-3_crKIT-2_crKIT-3_crCD81-1)

Plasmid

#217342

Purpose

crRNA array targeting CD81, B2M, KIT, CD55

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Inserts

crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pST-KiT

Plasmid

#44562

Purpose

for integrative inducible expression of mycobacterial proteins

Depositor

Vinay Nandicoori

Article

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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SI16-Kit a

Plasmid

#58948

Purpose

expresses recombinant mouse anti-Kit a receptor (zebrafish) IgG1 antibody in mammalian cells

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 . 8():76.

Insert

Kit receptor a mouse monoclonal antibody (kita Zebrafish)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
gCH198 (crCD55-4_crB2M-1_crB2M-3_crCLTA-4_crFOLH1-1_crCD151-3_crHBG-3_crKIT-2_crKIT-3_crCD81-1)

Plasmid

#217345

Purpose

crRNA array targeting CD55, B2M, CLTA, FOLH1, CD151, HBG1/HBG2, KIT, CD81

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Inserts

crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
crCLTA-4 gRNA: ggctctgcaacaccgcctagacc (CLTA Human)
crFOLH1-1 gRNA: gctccagacctggggtccagttt (FOLH1 Human)
crCD151-3 gRNA: cgggaggccgcacccaccgcctg (CD151 Human)
crHBG-3 gRNA: ttcttcatccctagccagccgcc (HBG1, HBG2 Human)
crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pC-KIT5

Plasmid

#118987

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Use

Tags

Expression

Mammalian

Mutation

mutK2-mutSP-K1

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pC-KIT1

Plasmid

#118983

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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eDrcKIT

Plasmid

#184001

Purpose

Optically controlled eDrcKIT expression in mammalian cells

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrcKIT (KIT Human)

Use

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pC-KIT2

Plasmid

#118984

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Use

Tags

Expression

Mammalian

Mutation

mutK2-SP-K1

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pC-KIT4

Plasmid

#118986

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Use

Tags

Expression

Mammalian

Mutation

K2-SP-mutK1

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pC-KIT6

Plasmid

#118988

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Use

Tags

Expression

Mammalian

Mutation

mutK2-SP-mutK1

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pC-KIT8

Plasmid

#118990

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Use

Tags

Expression

Mammalian

Mutation

mutK2-mutSP-mutK1

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_KIT

Plasmid

#183306

Purpose

All-in-One CRISPRko system with a guide RNA that targets KIT gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

KIT

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pC-KIT3

Plasmid

#118985

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Use

Tags

Expression

Mammalian

Mutation

K2-mutSP-K1

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pC-KIT7

Plasmid

#118989

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Use

Tags

Expression

Mammalian

Mutation

K2-mutSP-mutK1

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
SI14-Kit b

Plasmid

#46304

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 . 8():76.

Insert

Kit receptor b mouse monoclonal antibody (kitb Zebrafish)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only
pCS2-zfKitlgb

Plasmid

#168200

Purpose

Express zebrafish Kitlgb, mRNA synthesis

Depositor

Petr Bartunek

Article

Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

Insert

Kitlgb (kitlgb Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Tags

Expression

Mammalian

Mutation

I85V, V222I, and D233N- please see depositor comm…

Promoter

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pSrTA1662-toolkit

Plasmid

#154372

Purpose

Expresses SrTA1662 in plants

Depositor

Brande Wulff

Article

Gaurav et al Nat Biotechnol. 2022 Mar;40(3):422-431.

Insert

SrTA1662

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Native promoter

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-zfKitlga

Plasmid

#168199

Purpose

Express zebrafish Kitlga, mRNA synthesis

Depositor

Petr Bartunek

Article

Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

Insert

Kitlga (kitlga Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Tags

Expression

Mammalian

Mutation

F198L - please see depositor comments

Promoter

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-gRNA:kit-1

Plasmid

#60969

Purpose

Expression vector of rat Kit-1 guide RNA

Depositor

Tomoji Mashimo

Article

Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

Insert

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA (Kit Rat)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only