Showing: 41 - 60 of 72 results
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Plasmid#83413PurposeExpressed N-term tagged UnaG on Sec61BDepositorInsertUnaG-Sec61B (SEC61B Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(+)-SEC61B-mEGFP
Plasmid#115910Purposedonor to insert EGFP at the SEC61B locus in human cellsDepositorInsertSEC61B-mEGFP (SEC61B Human)
UseDonor templateTagsEGFPExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(+)-SEC61B-dTomato
Plasmid#115913Purposedonor to insert dTomato at the SEC61B locus in human cellsDepositorInsertSEC61B-dTomato (SEC61B Human)
UseDonor templateTagsdTomatoExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-SEC61B-dTomato
Plasmid#115920Purposedonor to insert dTomato at the SEC61B locus in human cellsDepositorInsertSEC61B-dTomato
UseDonor templateTagsdTomatoExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-SEC61B-mEGFP
Plasmid#115921Purposedonor to insert mEGFP at the SEC61B locus in human cellsDepositorInsertSEC61B-mEGFP
UseDonor templateTagsEGFPExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hSEC61B-C-PEN2
Plasmid#183648PurposeLentiviral expression of a ER-residential PEN2.DepositorUseLentiviralTagsHAExpressionMutationWTPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
FRB-mCherry-Sec61β
Plasmid#186574PurposeExpression of FRB-mCherry-tagged ER protein Sec61beta in mammalian cellsDepositorInsertSEC61B (SEC61B Human)
UseTagsFRB-mCherryExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLV-GFP-Sec61β
Plasmid#186597PurposeThird generation lentiviral vector expressing GFP-Sec61βDepositorInsertSec61β (SEC61B Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pADV-Sec61b-TurboID
Plasmid#179143PurposeRecombinant adenoviral plasmid for Sec61b-TurboID expressionDepositorInsertSec61b-V5-TurboID
UseAdenoviralTagsV5ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
AAVS1 EGFP-Sec61B
Plasmid#207553PurposeHomologous recombination donor to integrate and expression cassette for EGFP-Sec61B in the AAVS1 locus of human cellsDepositorInsertTET inducible EGFP-Sec61B
UseTagsEGFPExpressionMammalianMutationPromoterEndogenousAvailabilityAcademic Institutions and Nonprofits only -
AAVS1 SNAP-Sec61B
Plasmid#207580PurposeHomologous recombination donor to integrate and expression cassette for SNAPtag-Sec61B in the AAVS1 locus of human cellsDepositorInsertTET inducible SNAPTag-Sec61B
UseTagsSNAPTAGExpressionMammalianMutationPromoterEndogenousAvailabilityAcademic Institutions and Nonprofits only -
MCP-mCherry-SEC61B
Plasmid#207671PurposeTo express fluorescently labeled MS2 coat binding protein (MCP) fused with ER resident protein SEC61BDepositorInsertSEC61B (SEC61B Human)
UseTagsMCP-mCherryExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag
Plasmid#216250PurposeHDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlagDepositorInsertHDR template for human SEC61B
UseCRISPR; Hdr templateTagsBSD.P2A.miniIAA7.3xFlagExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Sec61 beta pAcGFP-C1 (802)
Plasmid#62008Purposemammalian expression of GFP-Sec61 betaDepositorInsertSec61 beta (SEC61B Human)
UseTagsAcGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCDNA3/flag-pbira(1-g78)-sec61b
Plasmid#154455Purposeexpresses flag-pbira(1-g78)-sec61b/biochemical assayDepositorInsertflag-pbira(1-g78)-sec61b (SEC61B Human)
UseTagsExpressionMammalianMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
Lenti-AcGFP-Sec61-IRES-Blast
Plasmid#154009PurposeLentiviral vector plasmid for integration and constitutive expression of AcGFP fused to Sec61 in mammalian cells – gift from D. Gerlich (IMBA, Vienna)DepositorInsertacGFP-Sec61 (SEC61B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCA22-SEC61B 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)
Plasmid#199453PurposeCRISPR donor plasmid to insert TriTag (mTagBFP) into the C-terminus of human SEC61B geneDepositorInsertHDR donor template
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-4xGFP11-SEC61B-IRES-Hygro
Plasmid#211468PurposeLentivirus backbone expressing SEC61B protein tagged with 4 tandem GFP11 repeats at the N-terminalDepositorInsert4xGFP11-SEC61B
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailabilityAcademic Institutions and Nonprofits only -
pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro
Plasmid#216251PurposeExpress Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminusDepositorInsertCas9 and sgSEC61B.N1
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
TRAPa-3xFLAG-pCDNA
Plasmid#201963PurposeMammalian expression of Translocon-associated protein subunit alphaDepositorInsertSSR1 (SSR1 Human)
UseTags3xFLAG-tagExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 41 - 60 of 72 results