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Addgene
Showing: 81 - 100 of 839 results

  1. pRDB_181

    Plasmid
    #216102
    Purpose
    CRISPRi, EF1a-driven dCas9-KRAB (ZNF10) (Cas only)
    Depositor
    John Doench
    Insert
    Cas9 [Sp]
    Use
    CRISPR and Lentiviral; Assembled vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216102-plasmid-map-sequence-id-431379

    Enlarge

  2. pRDB_182

    Plasmid
    #216103
    Purpose
    CRISPRi, EF1a-driven dCas9-KRAB (ZIM3) (Cas only)
    Depositor
    John Doench
    Insert
    Cas9 [Sp]
    Use
    CRISPR and Lentiviral; Assembled vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216103-plasmid-map-sequence-id-431400

    Enlarge

  11. pET15b-ACPs (from S. pneumonia)

    Plasmid
    #63687
    Purpose
    Inducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniae
    Depositor
    Michael Johnson
    Insert
    acpS
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only

  12. FU_tetO_dCas9-VP192-T2A-EGFP

    Plasmid
    #121173
    Purpose
    Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.
    Depositor
    Timo Otonkoski
    Insert
    dCas9-VP192-T2A-EGFP
    Use
    CRISPR and Lentiviral; Inducible by doxycycline
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121173-plasmid-map-sequence-id-234995

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  13. AA023

    Plasmid
    #215931
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Insert
    U6_v1; sgCD81_v1 [Sp]; trRNA_v2 [Sp]; CS2
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215931-plasmid-map-sequence-id-431459

    Enlarge

  14. AA145

    Plasmid
    #215937
    Purpose
    Fragmid fragment: (guide cassette) guide expression; Chen F+E trRNA (preferred)
    Depositor
    John Doench
    Insert
    U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215937-plasmid-map-sequence-id-431395

    Enlarge

  15. AA232

    Plasmid
    #215940
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNA
    Depositor
    John Doench
    Insert
    U6_v1; sgCD47_v4 [Sp]; trRNA_v14 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215940-plasmid-map-sequence-id-431480

    Enlarge

  16. AA233

    Plasmid
    #215941
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNA
    Depositor
    John Doench
    Insert
    U6_v1; sgCD274_v1 [Sp]; trRNA_v14 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215941-plasmid-map-sequence-id-431532

    Enlarge

  17. AA238

    Plasmid
    #215942
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Insert
    U6_v1; sgCD47_v4 [Sp]; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215942-plasmid-map-sequence-id-431479

    Enlarge

  18. AA242

    Plasmid
    #215943
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Insert
    U6_v1; sgCD47_v1 [Sp]; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215943-plasmid-map-sequence-id-431495

    Enlarge

  19. AA243

    Plasmid
    #215944
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Insert
    U6_v1; sgCD47_v5 [Sp]; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215944-plasmid-map-sequence-id-431494

    Enlarge

  20. AA244

    Plasmid
    #215945
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Insert
    U6_v1; sgCD59_v1 [Sp]; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215945-plasmid-map-sequence-id-431525

    Enlarge

Showing: 81 - 100 of 839 results