We narrowed to 259 results for: T7 rna polymerase

1. pMJO-6 (minus T7 RNA polymerase binding site)

   Plasmid

   #31991

   Depositor

   Mark Osborn

   Article

   Osborn et al Hum Gene Ther. 2011 Jun 13.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Oct. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato

   Plasmid

   #138610

   Purpose

   Express TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backbone

   Depositor

   Fei Chen

   Article

   Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Inserts

   Hyperactive AID
   T7 RNA Polymerase
   tdTomato
   

   Expression

   Mammalian

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS4333 yeTadA1.0-T7 RNAP

   Plasmid

   #137735

   Purpose

   pCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable marker

   Depositor

   Christina Smolke

   Article

   Cravens et al Nat Commun. 2021 Mar 11;12(1):1579. doi:

   Insert

   yeTadA1.0-T7 RNAP
   

   Expression

   Bacterial and Yeast

   Mutation

   E366G (See depositor comment below)

   Available Since

   Jan. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-T7 RNA POL

   Plasmid

   #138352

   Purpose

   Expressing T7 RNA Polymerase in mammalian cells

   Depositor

   Baisong Lu

   Insert

   T7 RNA POL
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS314-T7-GFP

   Plasmid

   #33130

   Depositor

   Michael Rosbash

   Article

   Dower et al RNA. 2002 May;8(5):686-97.

   Insert

   GFP with TDH3 5' and 3' UTR (gfp )
   

   Expression

   Yeast

   Promoter

   T7

   Available Since

   Dec. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p6XHis-T7(P266L)

   Plasmid

   #174866

   Purpose

   Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.

   Depositor

   Anna Pyle

   Article

   Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.

   Insert

   T7 RNA polymerase
   

   Tags

   6XHis-tag

   Expression

   Bacterial

   Mutation

   P266L

   Promoter

   lac promoter

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEF-BOS-T7-CA150

   Plasmid

   #21924

   Depositor

   Mariano Garcia-Blanco

   Article

   Sune et al Mol Cell Biol. 1999 Jul . 19(7):4719-28.

   Insert

   CA150 (TCERG1 Human)
   

   Tags

   T7

   Expression

   Mammalian

   Available Since

   Sept. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pBP-T7RNAP

   Plasmid

   #74096

   Purpose

   T7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNA

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   T7 RNAP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   None

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p70a-T7RNAP

   Plasmid

   #105683

   Purpose

   Expresses T7 polymerase in cell-free TXTL.

   Depositor

   Chase Beisel

   Article

   Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

   Insert

   T7 polymerase
   

   Expression

   Bacterial

   Promoter

   OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pZB578_pB1H1_T7-RNAP_LARP-I

    Plasmid

    #228507

    Purpose

    Bacterial Hybrid Driver Plasmid

    Depositor

    Timothy Whitehead

    Article

    Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

    Insert

    T7 RNAP, LARP-I
    

    Use

    Synthetic Biology

    Tags

    Umu Degron, GTG-start codon

    Mutation

    R378K; S430P, N433T, S633P, F849I, F880Y; W727G, …

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSC101-pT7-T3RNAP

    Plasmid

    #140872

    Purpose

    T7 promoter driven synthesis of T3 RNA polymerase

    Depositor

    Neal Devaraj

    Article

    Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

    Insert

    T3 RNA polymerase
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pZB573_pB1H1_T7-RNAP_LARP-I5CHO_1

    Plasmid

    #228508

    Purpose

    Bacterial Hybrid Driver Plasmid

    Depositor

    Timothy Whitehead

    Article

    Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

    Insert

    T7 RNAP, LARP-I5CHO (#1)
    

    Use

    Synthetic Biology

    Tags

    Umu Degron, GTG-start codon

    Mutation

    R378K; S430P, N433T, S633P, F849I, F880Y; W727G, …

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. T7RNAP Cyslight

    Plasmid

    #205077

    Purpose

    T7RNAP protein expression

    Depositor

    Smita Patel

    Article

    Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

    Insert

    T7RNAP Cyslight (T7p07 Bacteriophage T7)
    

    Expression

    Bacterial

    Mutation

    C125S, C347S, C510S, C515S, C530S, C723S

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. T7RNAP Cyslight V174C

    Plasmid

    #205078

    Purpose

    T7RNAP protein expression

    Depositor

    Smita Patel

    Article

    Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

    Insert

    T7RNAP Cyslight V174C (T7p07 Bacteriophage T7)
    

    Expression

    Bacterial

    Mutation

    C125S, C347S, C510S, C515S, C530S, C723S V174C

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT7RNAPNev-NB1

    Plasmid

    #216336

    Purpose

    Arabinose-inducible PBad promoter driving expression of the evolved N-terminal portion (residues 1-179) of T7RNAP translationally fused to anti-eGFP nanobody NB1

    Depositor

    Mark Styczynski

    Article

    McSweeney et al Sci Adv. 2025 Feb 21;11(8):eado6280. doi

    Insert

    T7RNAPN-NB1 fusion
    

    Expression

    Bacterial

    Available Since

    July 3, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pQE-WT-RGVG-M6

    Plasmid

    #70172

    Purpose

    Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

    Depositor

    Andrew Ellington

    Article

    Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

    Insert

    RGVG-M6 T7 RNA polymerase
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T5/Lac

    Available Since

    Dec. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pEcRNAP6

    Plasmid

    #128940

    Purpose

    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z

    Depositor

    Seth Darst

    Article

    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:

    Inserts

    RpoA (rpoA E. coli)
    RpoB
    RpoC-ppx(His)10
    RpoZ
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    PreScission protease site (GE Healthcare; LEVLFQG…

    Promoter

    T7

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-T7pol

    Plasmid

    #59926

    Purpose

    Expression vector for T7 RNA polymerase gene

    Depositor

    Ian Wickersham

    Insert

    T7 RNA polymerase
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEcRNAP5

    Plasmid

    #128939

    Purpose

    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, Z

    Depositor

    Seth Darst

    Article

    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:

    Inserts

    RpoAppx234-241-(His)10 (rpoA Escherichia coli)
    RpoB
    RpoC
    RpoZ
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Residues 234-241 are substituted for a PreScissio…

    Promoter

    T7

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

    Plasmid

    #217808

    Purpose

    Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge