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pST_APOE_HUMAN_E114I (pBS0839)

Plasmid

#185274

Purpose

For the mammalian expression of the human protein APOE_HUMAN_E114I. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_E114I

Use

Tags

Expression

Mammalian

Mutation

E114I

Promoter

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_D125I (pBS0840)

Plasmid

#185275

Purpose

For the mammalian expression of the human protein APOE_HUMAN_D125I. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_D125I

Use

Tags

Expression

Mammalian

Mutation

D125I

Promoter

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_R163C (pBS0842)

Plasmid

#185276

Purpose

For the mammalian expression of the human protein APOE_HUMAN_R163C. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_R163C

Use

Tags

Expression

Mammalian

Mutation

R163C

Promoter

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_K164E (pBS0843)

Plasmid

#185277

Purpose

For the mammalian expression of the human protein APOE_HUMAN_K164E. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_K164E

Use

Tags

Expression

Mammalian

Mutation

K164E

Promoter

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_V153A_G131S_11s (pBS0850)

Plasmid

#185281

Purpose

For the mammalian expression of the human protein APOE_HUMAN_V153A_G131S_11s. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_V153A_G131S_11s

Use

Tags

Expression

Mammalian

Mutation

V153A, G131S

Promoter

Availability

Academic Institutions and Nonprofits only

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pEJS2217_Nme2-rAPOBEC-nt

Plasmid

#201523

Purpose

Mammalian expression of N-terminally fused Nme2-rAPOBEC-nt

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-rAPOBEC-nt

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS2214_Nme2-rAPOBEC-i1

Plasmid

#201524

Purpose

Mammalian expression of domain inlaid Nme2-rAPOBEC-i1

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-rAPOBEC-i1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pEJS2215_Nme2-rAPOBEC-i7

Plasmid

#201525

Purpose

Mammalian expression of domain inlaid Nme2-rAPOBEC-i7

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-rAPOBEC-i7

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pEJS2216_Nme2-rAPOBEC-i8

Plasmid

#201526

Purpose

Mammalian expression of domain inlaid Nme2-rAPOBEC-i8

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-rAPOBEC-i8

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pAct-dCBEevoAPOBEC1

Plasmid

#195514

Purpose

Ubiquitous expression of dCBEevoAPOBEC1 in Drosophila

Depositor

Michael Boutros

Article

Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

Insert

dCBE-evoAPOBEC1

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

act5C

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApoE2

Plasmid

#197146

Purpose

Expresses ApoE2 in Mammalian cells

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

ApoE2 (APOE Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-ApoE3

Plasmid

#197147

Purpose

Expresses ApoE3 in Mammalian cell

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

ApoE3 (APOE Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-ApoE4

Plasmid

#197148

Purpose

Expresses ApoE4 in Mammalian cell

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

ApoE4 (APOE Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_AC-vapol

Plasmid

#202118

Purpose

Level 0 plasmid containing the promoter from gene 47434 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

vapol promoter

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_EF-vapol

Plasmid

#202174

Purpose

Level 0 plasmid containing a terminator sequence from gene 47434 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

vapol terminator

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

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psiCheck-ApoE3'UTR

Plasmid

#41846

Depositor

Sohail Tavazoie

Article

Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

Insert

ApoE 3'UTR (APOE Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
APOH-bio-His

Plasmid

#52177

Purpose

Expresses full-length Beta-2-glycoprotein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

APOH (APOH Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pTC-ApoE-Tet

Plasmid

#85578

Purpose

Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (ApoE.HCR.hAAT promoter ) in mouse liver

Depositor

Ursula Ehmer

Article

Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg

Insert

CreER

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

ApoE.HCR.hAAT

Availability

Academic Institutions and Nonprofits only

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pXPA-fapO-RgTALsyn

Plasmid

#100946

Purpose

Expression of Tyrosine Ammonia Lyase from constitutive GAP promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

GAP (Constitutive)

Availability

Academic Institutions and Nonprofits only

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pEGFP-Alus-Apobec3G

Plasmid

#92348

Purpose

Plasmid for expression of GFP gene with a Apobec3G gene intron derived Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Apobec3G gene intron derived Alu element containing 3'UTR (APOBEC3G Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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