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Addgene
Showing: 101 - 120 of 4498 results
  1. pHR_SFFV*/Puro2a-GFP

    Plasmid
    #198222
    Purpose
    Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFP
    Depositor
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    Codon-optimized puromycin resistance gene, follow…
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pAH-CTX1-rhadCas9

    Plasmid
    #129391
    Purpose
    Derived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.
    Depositor
    Insert
    Burkholderia cenocepacia codon-optimized dCas9
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Entire dCas9 codon optimized for the GC-rich B. c…
    Promoter
    PrhaBAD
    Availability
    Academic Institutions and Nonprofits only
  3. pCAGGS SARS-CoV-2 BQ.1.1 Spike

    Plasmid
    #193710
    Purpose
    Expresses codon-optimized full length SARS-CoV-2 BQ.1.1 Spike
    Depositor
    Insert
    SARS-CoV-2 BQ.1.1 Spike (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains the following mutations: T19I, LPPA24S, …
    Promoter
    chicken β-actin promoter, CMV enhancer
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. pORFE0005

    Plasmid
    #112073
    Purpose
    AtDeadCAS9 D10A H840A without stop codon module CDS1ns
    Insert
    AtDeadCAS9 D10A H840A without stop codon
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pET-HIS-Sangamo

    Plasmid
    #40786
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    N Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  6. pET-Sangamo-HIS

    Plasmid
    #40787
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    C Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus,Codon Optimized FokI Endonuclease
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  7. pFastBac_Dual_SNF2L-opt_p48

    Plasmid
    #102243
    Purpose
    Expression of human codon optimized human SNF2L and pRbAp48 from pFastBac dual
    Depositor
    Inserts
    SNF2L (SMARCA1 Human)
    pRbAp48 (RBBP4 Human)
    Use
    Tags
    Expression
    Insect
    Mutation
    codon optimized for expression
    Promoter
    Polyhedrin and p10
    Availability
    Industry, Academic Institutions, and Nonprofits
  8. pCAG-BE4max-P2A-EGFP (pRZ33)

    Plasmid
    #140083
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP
    Depositor
    Insert
    Human codon optimized pCAG BE4max with P2A-EGFP
    Use
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A (SpCas9)
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
  9. pLSU1/t35s[TMV]:mNeonGreen-I{PIV-198}:NosT

    Plasmid
    #212166
    Purpose
    This binary vector expresses mNeonGreen containing the PIV intron (198bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTR
    Depositor
    Insert
    Tomato codon optimized version of mNeonGreen-I{PIV-198}
    Use
    Tags
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato and PIV int…
    Promoter
    truncated 35sCaMV
    Availability
    Academic Institutions and Nonprofits only
  10. pLSU1/t35s[TMV]:mNeonGreen-I{PIV-216}:NosT

    Plasmid
    #212167
    Purpose
    This binary vector expresses mNeonGreen containing the PIV intron (216bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTR
    Depositor
    Insert
    Tomato codon optimized version of mNeonGreen-I{PIV-216}
    Use
    Tags
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato and PIV int…
    Promoter
    truncated 35sCaMV
    Availability
    Academic Institutions and Nonprofits only
  11. pLSU1/t35s[TMV]:mNeonGreen-I{PIV-390}:NosT

    Plasmid
    #212168
    Purpose
    This binary vector expresses mNeonGreen containing the PIV intron (390bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTR
    Depositor
    Insert
    Tomato codon optimized version of mNeonGreen-I{PIV-390}
    Use
    Tags
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato and PIV int…
    Promoter
    truncated 35sCaMV
    Availability
    Academic Institutions and Nonprofits only
  12. pCRISPR-aBEST

    Plasmid
    #131464
    Purpose
    Expression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes
    Depositor
    Inserts
    Streptomyces codon optimized spCas9n (D10A)
    streptomyces codon optimized tadA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    tipA
    Availability
    Academic Institutions and Nonprofits only
  13. pBAD-bHs

    Plasmid
    #134259
    Purpose
    For L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tag
    Depositor
    Insert
    Hyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    codon optimized for Salmonella typhimurium
    Promoter
    AraC
    Availability
    Academic Institutions and Nonprofits only
  14. pCAGGS SARS-CoV-2 BA.2.75 Spike

    Plasmid
    #190012
    Purpose
    Expresses codon-optimized full length SARS-CoV-2 BA.2.75 Spike
    Depositor
    Insert
    SARS-CoV-2 BA.2.75 Spike (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains the BA.2 mutations (T19I, LPPA24S, G142D…
    Promoter
    chicken β-actin promoter, CMV enhancer
    Availability
    Industry, Academic Institutions, and Nonprofits
  15. pmCellFree_KA1_SARS-COV-2_ORF7a

    Plasmid
    #169393
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF7a with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _ORF7a (ORF7a SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Availability
    Academic Institutions and Nonprofits only
Showing: 101 - 120 of 4498 results