We narrowed to 450 results for: crispr library

1. library backbone 3'LTR gRNA and barcode

   Plasmid

   #127397

   Purpose

   Digestion by BstXI and BamHI to release the backbone

   Depositor

   Xiaowei Zhuang

   Article

   Wang et al Proc Natl Acad Sci U S A. 2019 May 28;11

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Puro-mCherry

   Expression

   Mutation

   Promoter

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Gly-tRNA-gRNA scaffold for library 2

   Plasmid

   #192365

   Purpose

   tRNA sequence provider (for checkpoint library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Insert

   Gly tRNA-gRNA scaffold
   

   Use

   For golden gate assembly

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Pro-tRNA-gRNA scaffold for library 2

   Plasmid

   #192366

   Purpose

   tRNA sequence provider (for checkpoint library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Insert

   Pro tRNA-gRNA scaffold
   

   Use

   For golden gate assembly

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Gly-tRNA-gRNA scaffold for library 1

   Plasmid

   #192368

   Purpose

   tRNA sequence provider (for immune library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Insert

   Gly tRNA-gRNA scaffold
   

   Use

   For golden gate assembly

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Pro-tRNA-gRNA scaffold for library 1

   Plasmid

   #192369

   Purpose

   tRNA sequence provider (for immune library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Insert

   Pro tRNA-gRNA scaffold
   

   Use

   For golden gate assembly

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Gln-tRNA-gRNA scaffold for library 1&2

   Plasmid

   #192367

   Purpose

   tRNA sequence provider (for checkpoint and immune library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Insert

   Gln tRNA-gRNA scaffold
   

   Use

   For golden gate assembly

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Immune checkpoint (library 2) Backbone

   Plasmid

   #192363

   Purpose

   Plasmid vector for in vivo immune checkpoint screening library (for checkpoint library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. T cell activation (library 1) Backbone

   Plasmid

   #192364

   Purpose

   Plasmid vector for in vitro Jurkat activation screening (for immune library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)

   Plasmid

   #217306

   Purpose

   sgRNA mcherry + Puromycin

   Depositor

   Luke Gilbert

   Insert

   pU6-sgRNA, Ef1alpha-Puro-T2A-mcherry
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6, Ef1alpha

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-BFP)

    Plasmid

    #217305

    Purpose

    sgRNA BFP + Puromycin

    Depositor

    Luke Gilbert

    Insert

    pU6-sgRNA, Ef1alpha-Puro-T2A-BFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    U6, Ef1alpha

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cre-responsive Cas9 expressing transposon library vector

    Plasmid

    #164138

    Purpose

    Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6, EF-1a

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPRia-v2

    Plasmid

    #84832

    Purpose

    CRISPRi/a V2 library parental plasmid

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Inserts

    sgRNA
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-dual-CRISPR-U6-H1(pBP43)

    Plasmid

    #218930

    Purpose

    The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.

    Depositor

    Baoxu Pang

    Article

    Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 and H1

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenticrispr-wt-ltr-puro

    Plasmid

    #173428

    Purpose

    Expresses Cas9 and guide RNA, contains intact LTR

    Depositor

    Jakob Reiser

    Article

    Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

    Insert

    S. pyogenes sgRNA cassette
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPR v2

    Plasmid

    #52961

    Purpose

    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Inserts

    Cas9
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCRISPR_mCherry

    Plasmid

    #229078

    Purpose

    Small lentiviral backbone for CRISPR guide libraries, improved titre for hard to transduce cells

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1s

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiCRISPR_DKO

    Plasmid

    #183192

    Purpose

    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

    Depositor

    Lang Li

    Article

    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, mU6

    Available Since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Dual-CRISPR-Cas9-U6-H1 (pBP48)

    Plasmid

    #218932

    Purpose

    The backbone dual-CRISPR plasmid containing Cas9 that is used for making dual-CRISPR plasmids.

    Depositor

    Baoxu Pang

    Article

    Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    2A-Puro and 3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. CRISPRoff-mScarletI

    Plasmid

    #217783

    Purpose

    Expresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencing

    Depositor

    Adriano Aguzzi

    Article

    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

    Insert

    CRISPRoff-mScarletI (DNMT3A-DNMT3L-dCas9-mScarletI-KRAB)
    

    Use

    Tags

    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and mScarletI

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVC-CRISPR

    Plasmid

    #120369

    Purpose

    Plasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing library

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Inserts

    CRISPR1
    CRISPR2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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