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Showing: 1 - 20 of 92 results

  8. pDNA2.0 6H-TEV-KRAS G13D

    Plasmid
    #111851
    Purpose
    Expresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.
    Depositor
    Kenneth Westover
    Article
    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.
    Insert
    KRAS G13D (KRAS Human)
    Use
    Tags
    6xHis-tag and TEV protease cleavage sequence
    Expression
    Bacterial
    Mutation
    Changed Glycine 13 to Aspartic Acid
    Promoter
    T5
    Availability
    Academic Institutions and Nonprofits only
    111851-plasmid-map-sequence-id-213465

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  10. pDNA2.0 6H-FLAG-TEV-KRAS G12C

    Plasmid
    #111848
    Purpose
    Expresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.
    Depositor
    Kenneth Westover
    Article
    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.
    Insert
    KRAS G12C (KRAS Human)
    Use
    Tags
    6xHis-tag, FLAG-tag, and TEV protease cleavage se…
    Expression
    Bacterial
    Mutation
    Changed Glycine 12 to Cysteine
    Promoter
    T5
    Availability
    Academic Institutions and Nonprofits only
    111848-plasmid-map-sequence-id-213454

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  12. pcDNA216-CoV2-RBD-alpha variant

    Plasmid
    #194599
    Purpose
    Mammalian expression of RBD domain from SARS-CoV-2 alpha
    Depositor
    Ben Murrell
    Insert
    CoV2-RBD-alpha variant (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    194599-plasmid-map-sequence-id-381522

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  13. pcDNA216-CoV2-RBD-gamma variant

    Plasmid
    #194600
    Purpose
    Mammalian expression of RBD domain from SARS-CoV-2 gamma
    Depositor
    Ben Murrell
    Insert
    CoV2-RBD-gamma variant (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    194600-plasmid-map-sequence-id-381528

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  14. pcDNA216-CoV2-RBD-delta variant

    Plasmid
    #194601
    Purpose
    Mammalian expression of RBD domain from SARS-CoV-2 delta
    Depositor
    Ben Murrell
    Insert
    CoV2-RBD-delta variant (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    194601-plasmid-map-sequence-id-381523

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  18. pJ151-HDR

    Plasmid
    #113631
    Purpose
    Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.
    Depositor
    Alex Kentsis
    Inserts
    Left homology arm
    Puromycin resistance gene
    Venus fluorescence gene
    Thymidine kinase
    Right homology arm
    Use
    Cre/Lox; Homology recombination selection vector
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    EF1a promoter, EF1a promoter (T2A cleaving sequen…
    Availability
    Academic Institutions and Nonprofits only
    113631-plasmid-map-sequence-id-222216

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  19. pJ251-GERC

    Plasmid
    #47441
    Purpose
    Expresses mCherry and sfGFP from divergent promoters. The backbone is cloned into DNA2.0 pJ251, and the design is based off of pZS2-123.
    Depositor
    George Church
    Insert
    Gene Expression Reporter Construct
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    47441-plasmid-map-sequence-id-67511

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  20. pScarlessHD-DsRed

    Plasmid
    #64703
    Purpose
    Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini.
    Depositor
    Kate O'Connor-Giles
    Insert
    piggyBac-3xP3-DsRed
    Use
    Tags
    Expression
    Mutation
    Promoter
    3xP3
    Availability
    Academic Institutions and Nonprofits only
    64703-plasmid-map-sequence-id-108921

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Showing: 1 - 20 of 92 results