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Addgene
Showing: 1 - 20 of 121 results

  1. FGB

    Plasmid
    #52030
    Purpose
    Expresses full-length Fibrinogen beta chain precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    FGB (FGB Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Stop Codon at amino acid 492, before CD4, bio and…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    52030-plasmid-map-sequence-id-80105

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  7. pET28a-FgßF3-ddFLN4-HIS-ybbr

    Plasmid
    #101743
    Purpose
    mutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces
    Depositor
    Hermann Gaub
    Article
    Milles et al Science. 2018 Mar 30;359(6383):1527-1533
    Insert
    FgßF3 peptide, ddFLN4 fingerprint (FGB Human, Dictyostelium discoideum)
    Use
    Tags
    HIS and ybbr
    Expression
    Bacterial
    Mutation
    C18S in ddFLN4, added 9F in FgßF3
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    101743-plasmid-map-sequence-id-199091

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  14. pFGL815

    Plasmid
    #52322
    Purpose
    A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 69-bp MCS (multiple cloning site) which contains 12 unique restriction sites.
    Depositor
    Naweed Naqvi
    Article
    Yang et al Fungal Genet Biol. 2014 Apr 24. pii: S10
    Type
    Empty backbone
    Use
    Agrobacterium
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    52322-plasmid-map-sequence-id-81025

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  15. pPEE13

    Plasmid
    #128359
    Purpose
    Recycable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mCherry ORF and a flexable linker at the 5' end.
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Insert
    Acetamidase (amdS)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    TEF1
    Availability
    Academic Institutions and Nonprofits only
    128359-plasmid-map-sequence-id-257614

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  16. pPEE16

    Plasmid
    #128360
    Purpose
    Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 1, a small gene-free region. Contains the amdS2 counterselectable marker.
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Type
    Empty backbone
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    TEF1
    Availability
    Academic Institutions and Nonprofits only
    128360-plasmid-map-sequence-id-254521

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  17. pPEE19

    Plasmid
    #128362
    Purpose
    Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 3, a small gene-free region. Contains the amdS2 counterselectable marker.
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Type
    Empty backbone
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    TEF1
    Availability
    Academic Institutions and Nonprofits only
    128362-plasmid-map-sequence-id-254049

    Enlarge

  18. pPEE20

    Plasmid
    #128363
    Purpose
    Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 4, a small gene-free region. Contains the amdS2 counterselectable marker.
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Type
    Empty backbone
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    TEF1
    Availability
    Academic Institutions and Nonprofits only
    128363-plasmid-map-sequence-id-254548

    Enlarge

  19. pPEE21

    Plasmid
    #128364
    Purpose
    Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 5, a small gene-free region. Contains the amdS2 counterselectable marker.
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Type
    Empty backbone
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    TEF1
    Availability
    Academic Institutions and Nonprofits only
    128364-plasmid-map-sequence-id-254518

    Enlarge

  20. pPEE22

    Plasmid
    #128365
    Purpose
    Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 6, a small gene-free region. Contains the amdS2 counterselectable marker.
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Type
    Empty backbone
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    TEF1
    Availability
    Academic Institutions and Nonprofits only
    128365-plasmid-map-sequence-id-254576

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Showing: 1 - 20 of 121 results