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Plasmid#199715PurposeExpresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.DepositorInsertmCherry-LgBiT-FLAG-NES
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad-LgBiT-PhoCl1-SmBiT-MBP
Plasmid#164034Purposeluciferase-based PhoCl screening constructDepositorInsertLgBiT-PhoCl1-SmBiT-MBP
UseTagsHis tag, T7 tag, Xpress TagExpressionBacterialMutationPromoteraraBad promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQC.Flip-nluc(LgBiT1-8)CP[Casp3A]-Fluc
Plasmid#200123PurposeRetroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A] protease reporter and P2A firefly luciferase in mammalian cellsDepositorInsertFlip-nluc(LgBiT1-8)CP[Casp3A] P2A firefly luciferase
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCh-LgBiT-2A-EGFP-Vin-PILATeS
Plasmid#216761PurposePiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS (700 pM) in mammalian cells. Requires transposaseDepositorInsertmCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT
Plasmid#216763PurposePiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposaseDepositorInsertmCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQC.Flip-nluc(LgBiT1-8)CP[CoVA]-Fluc
Plasmid#200125PurposeRetroviral expression of Flip-nluc(LgBiT1-8)CP[CoVA] protease reporter and P2A firefly luciferase in mammalian cellsDepositorInsertFlip-nluc(LgBiT1-8)CP[CoVA] P2A firefly luciferase
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-eGFP-P2A-LgBiT-NLS
Plasmid#187834PurposeExpression of eGFP-P2A-LgBiT-NLS from human synapsin promoter via adeno-associated virus for neuronal expression.DepositorInserteGFP-P2A-LgBiT-NLS
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQC.Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)]-Fluc
Plasmid#200124PurposeRetroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] protease reporter and P2A firefly luciferase in mammalian cellsDepositorInsertFlip-nluc(LgBiT1-8)CP[Casp3A(D5A)] P2A firefly luciferase
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(SA)]-Fluc
Plasmid#200128PurposeRetroviral expression of SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(SA)] protease reporter and P2A firefly luciferase in mammalian cellsDepositorInsertFlip-nluc(LgBiT1-8)CP[CoVA(SA)] P2A firefly luciferase
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA]-Fluc
Plasmid#200126PurposeRetroviral expression of SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA] protease reporter and P2A firefly luciferase in mammalian cellsDepositorInsertFlip-nluc(LgBiT1-8)CP[CoVA] P2A firefly luciferase
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)]-Fluc
Plasmid#200127PurposeRetroviral expression of SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)] protease reporter and P2A firefly luciferase in mammalian cellsDepositorInsertFlip-nluc(LgBiT1-8)CP[CoVA(Q5A)] P2A firefly luciferase
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAF256
Plasmid#105496PurposepRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and LgBiTDepositorInsertHupA-SmBiT/LgBiT
UseAtc-dependent expression in c. difficileTagsExpressionMutationPromoterPtetAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAF257
Plasmid#105497PurposepRPF185 derived plasmid encoding an anhydrotetracyclin inducible SmBiT and HupA-LgBiTDepositorInsertSmBiT/HupA-LgBiT
UseAtc-dependent expression in c. difficileTagsExpressionMutationPromoterPtetAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAP118
Plasmid#105495PurposepRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and HupA-LgBiT fusionsDepositorInsertHupA-SmBiT/HupA-LgBiT
UseAtc-dependent expression in c. difficileTagsExpressionMutationPromoterPtetAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWP001
Plasmid#178596PurposepAF256 derived plasmid encoding a xylose inducible CD16/50L CBD-SmBiT and LgBiTDepositorInsertCBD-SmBiT/LgBiT
UseTagsExpressionBacterialMutationPromoterPxylAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαq-LgB97
Plasmid#134360PurposeNanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.DepositorInsertGαq-LgB97 (GNAQ Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK172-6xHis-0N4R-P301S-tau-HiBiT
Plasmid#187835PurposeExpression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.DepositorInsert6XHis-0N4R-P301S-tau-HiBiT
UseTags6xHistidine and HiBiTExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi1-LgB91
Plasmid#134342PurposeNanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.DepositorInsertGαi1-LgB91 (GNAI1 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαs-LgB113
Plasmid#134362PurposeNanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.DepositorInsertGαs-LgB113 (GNAS Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi3-LgB91
Plasmid#134359PurposeNanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.DepositorInsertGαi3-LgB91 (GNAI3 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only