Addgene

Showing: 1 - 20 of 24 results

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Man2a1-myc

Plasmid

#163648

Purpose

Expresses myc tagged Man2a1 in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

mannosidase alpha class 2A member 1 (MAN2A1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Silent mutation was made to introduce a XbaI rest…

Promoter

Availability

Academic Institutions and Nonprofits only

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Man2a1-GFP

Plasmid

#163649

Purpose

Expresses GFP tagged Man2a1 in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

mannosidase alpha class 2A member 1 (MAN2A1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Silent mutation was made to introduce a XbaI rest…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.1 - circMAN2A1

Plasmid

#198837

Purpose

This contains the 400nt isoform of exons two and three from MAN2A1 circular RNA, 3X Flag tag inserted in exon two

Depositor

Stefan Stamm

Article

Arizaca Maquera et al Front Mol Neurosci. 2023 May 5;16:114107

Insert

circular mannosidase alpha class 2A member 1

Use

Tags

Expression

Mammalian

Mutation

circular RNA composed by 2 exons

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pPN112

Plasmid

#91639

Purpose

Express sgRNA targeting human MAN2A1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

MAN2A1 (MAN2A1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
pPN113

Plasmid

#91640

Purpose

Express sgRNA targeting human MAN2A1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

MAN2A1 (MAN2A1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF-ManII-GFP

Plasmid

#160905

Purpose

Expresses the Golgi marker ManII-GFP in mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

MAN2A1 (MAN2A1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF-1a

Availability

Academic Institutions and Nonprofits only

Enlarge
mPlum-MannII-N-10

Plasmid

#55984

Purpose

Localization: Mannosidase/Golgi, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Insert

MannII (Man2a1 Mouse)

Use

Tags

mPlum

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
mEos3.2-MannII-N-10

Plasmid

#57467

Purpose

Localization: Mannosidase/Golgi, Excitation: 507 / 572, Emission: 516 / 580

Depositor

Michael Davidson

Insert

MannII (Man2a1 Mouse)

Use

Tags

mEos3.2

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
mEos4b-MannII-N-10

Plasmid

#57513

Purpose

Localization: Mannosidase/Golgi, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Loren Looger, Michael Davidson

Insert

MannII (Man2a1 Mouse)

Use

Tags

mEos4b

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
PS-CFP-MannII-N-10

Plasmid

#57205

Purpose

Localization: Actin Capping Protein , Excitation: 400 / 490, Emission: 468 / 511

Depositor

Michael Davidson

Insert

MannII (Man2a1 Mouse)

Use

Tags

PS-CFP

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
StrKDEL-IRES-ManII-mScarlet-i

Plasmid

#117274

Purpose

Expresses an Str-KDEL Hook for the RUSH system and separately mannosidase II-mScarlet-i

Depositor

David Stephens

Article

McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180

Inserts

partial sequence of mannosidase II (Man2a1 Mouse)
Streptavidin-KDEL

Use

Tags

mScarlet-i

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV and IRES

Availability

Academic Institutions and Nonprofits only

Enlarge
Str-KDEL_ManII-SBP-EGFP

Plasmid

#65252

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Str-KDEL_ManII-SBP-mCherry

Plasmid

#65253

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Str-KDEL_ManII-SBP-tagBFP

Plasmid

#65254

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Ii-Str_ManII-SBP-EGFP

Plasmid

#65255

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated Mannosidase II

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Ii-Str_ManII-SBP-mCherry

Plasmid

#65256

Purpose

synchronize trafficking of ManII from the ER

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated Mannosidase II

Use

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Ii-Str_ManII-SBP-tagBFP

Plasmid

#65257

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated Mannosidase II

Use

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
ManII-SBP-EGFP_puromycin

Plasmid

#65260

Purpose

ManII reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated Mannosidae II

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
ManII-SBP-mCherry_puromycin

Plasmid

#65261

Purpose

ManII reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated Mannosidae II

Use

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Emerald-MannII-N-10

Plasmid

#54150

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

MannII (Man2a1 Mouse)

Use

Tags

Emerald

Expression

Mammalian

Mutation

aa 1-112 of MannII aa 1-112 of MannII

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits