Showing: 1 - 16 of 16 results
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Plasmid#132253PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertOCA2 (OCA2 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-OCA2_STOP
Plasmid#161419PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertOCA2 (OCA2 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
MELOPS
Plasmid#80151PurposeGenetically encoded melanosome-localized pH sensor.DepositorInsertOCA2 (OCA2 Human)
UseTagsmNectarine in first luminal loopExpressionMammalianMutationnonpathologic polymorphism D257A, pore mutation V…PromoterCMVAvailabilityIndustry, Academic Institutions, and Nonprofits -
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MLH1
Plasmid#28120PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMLH1:JMC01L-F09:C205690 (MLH1 Human)
UseTagsHis tag with TEV siteExpressionBacterialMutationcontains amino acid residues 1-347.PromoterAvailabilityAcademic Institutions and Nonprofits only -
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pCEP9 MLH1
Plasmid#16458DepositorInsertMLH1 (MLH1 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFB1_hMLH1
Plasmid#129426PurposeExpression human MLH1 protein using baculovirusDepositorInserthuman MLH1 (MLH1 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrin promoterAvailabilityAcademic Institutions and Nonprofits only -
373_MLH1 in pmiRGlo
Plasmid#78133Purposeluciferase reporter for miRNA activityDepositorInsertMLH1 (MLH1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation3'UTR containing miRNA binding sitesPromoterPGKAvailabilityAcademic Institutions and Nonprofits only -
Lenti-CMV-MLH1dn-IRES-puroR
Plasmid#207354PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).DepositorInsertMutL homolog 1 (MLH1 Human)
UseLentiviralTagsExpressionMutationΔ754-756 dominant negative mutantPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-hMLH1dn-Hygro
Plasmid#195512PurposeLentiviral expression plasmid of hMLH1dn with P2A-HygroR markerDepositorInserthMLH1dn-P2A-HygroR (MLH1 Human)
UseLentiviralTagsP2AExpressionMammalianMutationHuman MLH1 del754-756PromoterEF-1aAvailabilityAcademic Institutions and Nonprofits only -
pT7-hMLH1dn for IVT
Plasmid#178114PurposeTemplate for in vitro transcription of human MLH1dnDepositorInserthuman MLH1dn (codon optimized) (MLH1 Human)
UseTemplate for in vitro transcriptionTagsExpressionMutationDetailed in manuscriptPromoterT7 (inactivated)AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-hMLH1dn (og codon)
Plasmid#174823PurposeMammalian expression of human MLH1dn (original codon usage)DepositorInserthuman MLH1dn (original codon usage) (MLH1 Human)
UseTagsExpressionMammalianMutationdeletion of residues 754-756PromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLV-gag-hMLH1dn
Plasmid#195513PurposeMMLV-Gag fused to hMLH1dn with TSTLLMENSS cleavage site between Gag-NES and hMLH1dn. For production of virus like particles with base-editor RNP cargo.DepositorInserthMLH1dn (MLH1 Human)
UseVirus like particleTagsFLAG, NES, and Protease cleavate siteExpressionMammalianMutationHuman MLH1 del754-756PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD051-sgMLH1-1
Plasmid#125776Purposeconstitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9DepositorInsertsgMLH1-1 (MLH1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD051-sgMLH1-2
Plasmid#125777Purposeconstitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9DepositorInsertsgMLH1-2 (MLH1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 16 of 16 results