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Showing: 1 - 14 of 14 results

  1. pGM1190

    Plasmid
    #69994
    Purpose
    OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces
    Depositor
    Günther Muth
    Inserts
    aac
    tsr
    rep
    sso
    TipA promoter
    dso
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    69994-plasmid-map-sequence-id-142397

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  2. pGM190

    Plasmid
    #69614
    Purpose
    inducible gene expression in Streptomyces
    Depositor
    Günther Muth
    Inserts
    aphII
    tsr
    rep
    sso
    TipA promoter
    dso
    Use
    Streptomyces- e. coli shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    69614-plasmid-map-sequence-id-132831

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  3. pGM1202

    Plasmid
    #69615
    Purpose
    inducible gene expression in Streptomyces, C-terminal His-tag encoding sequence
    Depositor
    Günther Muth
    Inserts
    aac
    tsr
    rep
    sso
    TipA promoter
    dso
    Use
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    69615-plasmid-map-sequence-id-129702

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  4. pGM190oriT

    Plasmid
    #115679
    Purpose
    E. coli-Streptomyces shuttle vector for gene expression
    Depositor
    Günther Muth
    Insert
    oriT, aphII, tsr
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tipA
    Availability
    Academic Institutions and Nonprofits only
    115679-plasmid-map-sequence-id-410983

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  5. pGM1192

    Plasmid
    #115683
    Purpose
    E. coli-Streptomyces shuttle vector for gene expression
    Depositor
    Günther Muth
    Insert
    mCherry, aac(3)IV, tsr
    Use
    Tags
    Strep-tagII on mCherry, codon optimized
    Expression
    Bacterial
    Mutation
    mCherry codon optimized for Streptomyces
    Promoter
    SF14*
    Availability
    Academic Institutions and Nonprofits only
    115683-plasmid-map-sequence-id-237128

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  6. pCRISPR-aBEST

    Plasmid
    #131464
    Purpose
    Expression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes
    Depositor
    Tilmann Weber
    Article
    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116
    Inserts
    Streptomyces codon optimized spCas9n (D10A)
    streptomyces codon optimized tadA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    tipA
    Availability
    Academic Institutions and Nonprofits only
    131464-plasmid-map-sequence-id-261686

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  9. pCRISPR-Cas3

    Plasmid
    #209427
    Purpose
    Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes
    Depositor
    Tilmann Weber
    Article
    Whitford et al bioRxiv 2023.05.09.539971
    Insert
    codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    PtipA; crRNA under control of PermE*
    Availability
    Academic Institutions and Nonprofits only
    209427-plasmid-map-sequence-id-416703

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  12. pCRISPR-mcBEST

    Plasmid
    #209416
    Purpose
    Plasmid for multiplexed cytosine base editing in streptomycetes
    Depositor
    Tilmann Weber
    Article
    Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2
    Inserts
    Codon optimized APOBEC1-nCas9-UGI fusion protein
    csy4 from Pseudomonas aeruginosa PAO1
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    base editing cassette: PtipA ; sgRNAs: PkasO* an…
    Availability
    Academic Institutions and Nonprofits only
    209416-plasmid-map-sequence-id-416613

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Showing: 1 - 14 of 14 results