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K-to-R mutant NPRL2(1-380) in pLJM60

Plasmid

#184562

Purpose

CMV-driven expression of an untagged mutant NPRL2 (core subunit of GATOR1) — native sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.

Depositor

Kacper Rogala

Article

Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

Insert

NPRL2 (NPRL2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

All lysines were mutated to arginines.

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pRL-12

Plasmid

#12231

Depositor

Sung-Hou Kim

Insert

pRas189 L12

Use

Tags

Expression

Bacterial

Mutation

Gly12 to Leu12

Promoter

Availability

Academic Institutions and Nonprofits only
pJ2072.2_CRISPRlator

Plasmid

#140674

Purpose

CRISPRlator

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

CRISPRlator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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hPRL1_1-169

Plasmid

#209792

Purpose

Bacterial expression of human PRL-1 without farnesylation motif

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-1

Insert

PRL-1 (PTP4A1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-169 only

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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hPRL1_1-169_C104D

Plasmid

#209793

Purpose

Bacterial expression of human PRL-1 C104D mutant without farnesylation motif

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-1

Insert

PRL-1 (PTP4A1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-169 only; C101D

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pRL-TK 4x wt

Plasmid

#11313

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pRL_GI_PCB28

Plasmid

#98745

Purpose

For phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.

Depositor

Bernd Müller-Röber

Article

Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919

Inserts

heme oxygenase 1 (TED4 Mustard Weed)
Phycocyanobilin:ferredoxin oxidoreductase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Codon optimized for Saccharomyces cerevisiae

Promoter

ADH1m and PGK1

Availability

Academic Institutions and Nonprofits only

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pRL1075

Plasmid

#70688

Purpose

Use of a vector with a conditionally lethal gene, sacB, to help transfer transposon mutations to a wild type strain

Depositor

Peter Wolk

Article

Black et al Mol Microbiol. 1993 Jul;9(1):77-84.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pRL128

Plasmid

#40180

Depositor

Eric Cascales

Article

Gueguen et al Appl Environ Microbiol. 2013 Jan;79(1):3

Inserts

Ptac-lacO
araC-pBAD

Use

Tags

Expression

Mutation

Promoter

BAD and tac

Availability

Academic Institutions and Nonprofits only

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pRL439

Plasmid

#70258

Purpose

Source of psbA promoter of Amaranthus hybridus, used as a strong promoter of cyanobacterial genes

Depositor

Peter Wolk

Article

Elhai et al Gene. 1988 Aug 15;68(1):119-38.

Insert

psbA promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
hPRL2_1-163

Plasmid

#209794

Purpose

Bacterial expression of human PRL-2 without farnesylation motif

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-1

Insert

PRL-2 (PTP4A2 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-163 only

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pRLH-12

Plasmid

#12232

Depositor

Sung-Hou Kim

Insert

pRas189 Leu12 truncated

Use

Tags

Expression

Bacterial

Mutation

truncated with HindIII to 171aa (from 189aa); Gly…

Promoter

Availability

Academic Institutions and Nonprofits only
pShuttle-PRL.Cre

Plasmid

#192934

Purpose

pShuttle.Cre encoding sgRNAs targeting Trp53, Rb1 and Rbl2 genes

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Insert

Trp53, Rb1, Rbl2 (Trp53, Rb1 Mouse)

Use

Gateway-compatible vector

Tags

Expression

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pAd-PRL.Cre

Plasmid

#192937

Purpose

Adenoviral expression vector encoding sgRNAs targeting Trp53, Rb1 and Rbl2 and Cre

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Inserts

Rb1 (Rb1 Mouse)
Trp53 (Trp53 Mouse)
Rbl2 (Rbl2 Mouse)

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pRL-TK let7 A

Plasmid

#11324

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation A from figure 4A of paper.

Promoter

Availability

Academic Institutions and Nonprofits only
pRL-TK let7 B

Plasmid

#11325

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation B from figure 4A of paper.

Promoter

Availability

Academic Institutions and Nonprofits only
pRL-TK let7 C

Plasmid

#11326

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation C from figure 4A of paper.

Promoter

Availability

Academic Institutions and Nonprofits only
pRL-TK let7 D

Plasmid

#11327

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation D from figure 4A of paper.

Promoter

Availability

Academic Institutions and Nonprofits only
pRL-TK let7 E

Plasmid

#11328

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation E from figure 4A of paper.

Promoter

Availability

Academic Institutions and Nonprofits only
pRL-TK let7 F

Plasmid

#11329

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation F from figure 4A of paper.

Promoter

Availability

Academic Institutions and Nonprofits only