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Addgene
Showing: 21 - 40 of 174 results
  1. PP-GNPNAT1

    Plasmid
    #25281
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    Tb11.01.2886:V5-L147
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pSMOX-mNG::LacI-V3

    Plasmid
    #210224
    Purpose
    Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.
    Depositor
    Insert
    mNeongreen Lac repressor fusion protein
    Use
    Trypanosoma brucei expression
    Tags
    Expression
    Mutation
    Promoter
    Pol II readthrough transcription after integration
    Availability
    Academic Institutions and Nonprofits only
  3. 5KO4 (PP-BRD19)

    Plasmid
    #101882
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    PP-BRD19
    Use
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  4. PP-HSP90 (3O6O)

    Plasmid
    #28153
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    HSP90
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    N-terminal domain
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. PP-PMM

    Plasmid
    #25202
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    Tb10.70.0370:M1-K245
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids M1-K245.
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. PP-UDPGP

    Plasmid
    #25285
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    Tb10.389.0330:L3-E485
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids L3-E485
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. pTrex-n-eGFP

    Plasmid
    #62544
    Purpose
    Expression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genome
    Depositor
    Insert
    eGFP
    Use
    (1)trypanosoma cruzi expression of egfp (2) genom…
    Tags
    None
    Expression
    Mutation
    None
    Promoter
    Ribo-HX1
    Availability
    Academic Institutions and Nonprofits only
  8. 5K29 (PP-BRD3)

    Plasmid
    #101883
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    PP-BRD3
    Use
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  9. Cas9/pTREX-n

    Plasmid
    #68708
    Purpose
    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.
    Depositor
    Insert
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    Use
    CRISPR
    Tags
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …
    Expression
    Mutation
    C4239T mutation that eliminates a BamHI restricti…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 21 - 40 of 174 results