Showing: 21 - 40 of 1436 results
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Plasmid#24199DepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsExpressionMammalianMutationY654FPromoterAvailabilityAcademic Institutions and Nonprofits only -
BcatMut-Tyr432
Plasmid#24200DepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsExpressionMammalianMutationY432FPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDule-3-nitroTyrosine (5B)
Plasmid#85498PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsNoneExpressionBacterialMutationY32H H70C D158S I159A L162RPromoterlpp (constitutive)AvailabilityAcademic Institutions and Nonprofits only -
pDule2-3-nitroTyrosine (5B)
Plasmid#85499PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsExpressionBacterialMutationY32H H70C D158S I159A L162RPromoterlpp (constitutive)AvailabilityAcademic Institutions and Nonprofits only -
piRFP-N3-Tyrosinase
Plasmid#80152PurposeMelanosome markerDepositorInsertTyrosinase (TYR Human)
UseTagsiRFP682ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-haloTyrRS C6
Plasmid#141174PurposeExpression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acidsDepositorInsertC6 HaloTyr tRNA synthetase
UseLentiviralTagsExpressionMammalianMutationL270S, Y271L, N311G, C313TPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pRS423-TyrSgH
Plasmid#163973PurposeHelper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoterDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
60-p3E_INTRON-EmptyRNAi
Plasmid#163380Purposep3E-Vector (Gateway R2-L3) for Tol2-kit cloning. Vector for performing RNAi (gene silencing - knockdown) in zebrafish via cloning and expression of synthetic miR(s).DepositorTypeEmpty backboneUseRNAiTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDule-Mb haloTyrRS C6
Plasmid#160377PurposeC6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.DepositorInsertC6 HaloTyrosine tRNA synthatase
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationL270S, Y271L, N311G, C313TPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDule2-Mb haloTyrRS C6
Plasmid#160378PurposeC6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.DepositorInsertC6 HaloTyrosine tRNA synthatase
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationL270S, Y271L, N311G, C313TPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDule-3-nitroTyrosine (A7)
Plasmid#174078PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsExpressionBacterialMutationY32H H70T D158H I159A L162RPromoterGlnRS (constitutive)AvailabilityAcademic Institutions and Nonprofits only -
pDule2-3-nitroTyrosine (A7)
Plasmid#174079PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsExpressionBacterialMutationY32H H70T D158H I159A L162RPromoterGlnRS (constitutive)AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ1A
Plasmid#197381PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-1 σ1A
UseTagsGAL4-DNA binding domain fragment, HA tag, and SV4…ExpressionYeastMutationContains an extra 42 nucleotides encoding 14 resi…PromoterADH1 and MET25AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Tyr-P(11)4
Plasmid#185784PurposeEncodes enzyme tyrosinase linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertTyr-P(11)4
UseTagsHis-tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ2
Plasmid#197415PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-2 σ2
UseTagsGAL4-DNA binding domain fragment, HA tag, and nuc…ExpressionYeastMutationencodes R42G substitution, contains silent substi…PromoterADH1 and MET25AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ3A
Plasmid#197513PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-3 σ3A
UseTagsGAL4-DNA binding domain fragment, HA tag, and nuc…ExpressionYeastMutationsilent substitution in codon 2 of tyrosinase tail…PromoterADH1 and MET25AvailabilityAcademic Institutions and Nonprofits only -
Silene_conica _TyrRS_MitoTP_pK7FWG2
Plasmid#202657PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from Silene conica cytosolic TyrRS predicted to be re-targeted to mitochondria
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Silene_vulgaris _TyrRS_MitoTP_pK7FWG2
Plasmid#202658PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from Silene vulgaris cytosolic TyrRS predicted to be re-targeted to mitochondria
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Tol2-ftyrp>TRAP
Plasmid#42299DepositorInsertEGFP-Rpl10a (rpl10a Zebrafish)
UseTagsEGFPExpressionMutationPromoterTakifugu rubripes tyrosine related protein 1 (tyr…AvailabilityAcademic Institutions and Nonprofits only -
pGEM-7Zf-TYR
Plasmid#32786DepositorInsertTyrosinase (TYR Human)
UseIn vitro transcriptionTagsExpressionMutationI181MPromoterT7AvailabilityAcademic Institutions and Nonprofits only
Showing: 21 - 40 of 1436 results