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Showing: 101 - 120 of 1439 results

  1. A1-LCD-20G+20S-12F+12Y

    Plasmid
    #178689
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Insert
    A1-LCD-20G+20S-12F+12Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty Gly residues replaced with Ser, twelve Phe…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    178689-plasmid-map-sequence-id-356275

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  2. A1-LCD+23G-23S+7F-7Y

    Plasmid
    #178690
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, seven Tyr replaced with Phe (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Insert
    A1-LCD+23G-23S+7F-7Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty-three Ser residues replaced with Gly, seve…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    178690-plasmid-map-sequence-id-356145

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  3. A1-LCD+23G-23S-12F+12Y

    Plasmid
    #178691
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Insert
    A1-LCD+23G-23S-12F+12Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty-three Ser residues replaced with Gly, twel…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    178691-plasmid-map-sequence-id-356195

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  6. pAcBac2.tR4-OMeYRS/GFP*

    Plasmid
    #50831
    Purpose
    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells
    Depositor
    Peter Schultz
    Article
    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11
    Inserts
    two-copy Tyr tRNA cassette
    EGFP*
    OMeYRS
    two copy Tyr tRNA cassette
    Use
    Baculoviral
    Tags
    His, Myc-6xHis, and WPRE
    Expression
    Insect
    Mutation
    Y39TAG and able to charge various unnatural amino…
    Promoter
    CAG, CMV, and U6 and H1
    Availability
    Academic Institutions and Nonprofits only
    50831-plasmid-map-sequence-id-193569

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  7. pCAS-mak10 gRNA

    Plasmid
    #160385
    Purpose
    Expresses guide RNA for targeting MAK10 in yeast
    Depositor
    John McCusker
    Insert
    mak10 gRNA
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    RNA pol III promoter (tRNA-Tyr)
    Availability
    Academic Institutions and Nonprofits only
    160385-plasmid-map-sequence-id-384166

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  8. A1-LCD Aro+

    Plasmid
    #169717
    Purpose
    Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids. PHE and TYR amino acids shuffled to optimize mixing.
    Depositor
    Tanja Mittag
    Article
    Martin et al Science. 2020 Feb 7;367(6478):694-699. d
    Insert
    hnRNPA1 (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    hnRNPA1-A Low complexity domain including amino a…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    169717-plasmid-map-sequence-id-334791

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  10. ROR2

    Plasmid
    #39183
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ROR2 (ROR2 Human)
    Use
    Baculovirus expression
    Tags
    His Tev
    Expression
    Mutation
    Promoter
    polyhedrin
    Availability
    Academic Institutions and Nonprofits only

  11. pJK525

    Plasmid
    #71601
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Trp97>Tyr mutant (AaPurE1fyw)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tryptopha…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71601-plasmid-map-sequence-id-131675

    Enlarge

  12. pJK526

    Plasmid
    #71602
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-97 to tyrosine, tryptophan-165…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71602-plasmid-map-sequence-id-131676

    Enlarge

  13. pJK665

    Plasmid
    #71614
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Trp97>Tyr mutant (AaPurE1fyw-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71614-plasmid-map-sequence-id-131692

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  14. pJK666

    Plasmid
    #71615
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine, tryptophan-97…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71615-plasmid-map-sequence-id-131694

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  15. pKD‐SepRS-­EFSep-­5x tRNASep (B40 OTS)

    Plasmid
    #52054
    Purpose
    Used in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)
    Depositor
    Jesse Rinehart
    Inserts
    SepRS
    EF-Sep
    5x tRNA Sep
    Use
    Tags
    Expression
    Bacterial
    Mutation
    His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…
    Promoter
    Ptac
    Availability
    Academic Institutions and Nonprofits only
    52054-plasmid-map-sequence-id-80170

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Showing: 101 - 120 of 1439 results