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We narrowed to 954 results for: App;

Showing: 1 - 50 of 954 results

  1. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... Tempel 30137 pCAX APP 695 APP CAG Alzheimer's Dennis Selkoe 30138 pCAX APP 751 APP CAG Alzheimer's Dennis...Selkoe 30143 pCAX APP delta CT APP CAG Alzheimer's Dennis Selkoe 30144 pCAX APP AENATA APP CAG Alzheimer'...Selkoe 30145 pCAX APP Swe/Ind APP CAG Alzheimer's Dennis Selkoe 30146 pCAX APP C99 APP CAG Alzheimer's ... 30147 pCAX APPs-695-alpha APP CAG Alzheimer's Dennis Selkoe 30149 pCAX APPs-751-alpha APP CAG Alzheimer's... 196696 HA-APP-mGFP APP HA, GFP CMV Alzheimer's Martino Calamai 196704 mCherry-APP-mGFP APP GFP, mCherry...196694 mBFP-APP-mGFP APP tagBFP2, GFP CMV Alzheimer's Martino Calamai 196695 mBFP-APPP1-mGFP APP tagBFP2,...196705 mCherry-APPP1-mGFP APP GFP, mCherry CMV Alzheimer's Martino Calamai 196706 AP-APP APP Avi, HA CMV ...

  2. Neurodegeneration Research Collection

    Type
    Collection
    ...Rep Methods. 2022 Mar 28. Follow APP processing and dynamics using APP and Bace1 plasmids . Capitini et...inherited genes: Presenilin 1, Presenilin 2, and APP gene.The majority (>90%) of individuals develop late-onset...symptoms appear between a person’s thirties and mid-sixties, or late-onset, where symptoms appear during... these disorders. An integrated study of the overlapping biology of these diseases, rather than focusing...are still poorly understood but several genes and mapped loci seem to be associated with the disease and...

  3. 5 Great Apps for Lab Life

    Type
    Blog Post
    ... FREE science lab apps that have been educational and great for lab life. These apps prevent you from ...spectrometry app. I digress. When I started learning proper cell culture technique, I tried to find an app that...I came across the Gibco Cell Culture app. Gibco organizes its app into four categories: tools, analytics... Tools app comes in handy when I use protocols that require restriction enzymes or PCR. The app contains...Hence, I’m including this MSDS app. The Chemical Safety Data Sheets app has a search function that allows...of the many apps available to help researchers in the lab. If you have a favorite lab app, please share...by guest blogger Sean D. Stacey. Opinions on the apps discussed in the article are those of the guest ...

  4. PhD Applications After COVID

    Type
    Blog Post
    ...previous nosy question! The Application Process Broadly speaking, the PhD application process tends to go something...steps in the application process and usually occurs in the few months leading up to applications opening. ...program. Most applications will recommend that you name two professors in your application that you intend...to continue the application process through a campus visit, a weekend where applications can get to know...strengthen your application and help you decide if a program is a good fit for you. The application process looks...if you’re anything like me, the process of even applying to one of these programs is almost as daunting...be, at times, very overwhelming. As someone who applied to PhD programs in Biology around the United States...

  5. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...technique published every week! One of the newest applications is a colorful system that uses fluorescently...Thoru Pederson’s lab, brings us one step closer to mapping the 4D nucleome, the organization of the nucleus...activity, catalytically dead Cas9 (dCas9) has many applications, the most well-known being transcriptional activation... 9 and 13. They next turned their attention to mapping pairs of intrachromosomal loci. The technique successfully...authors’ knowledge, this work represents the first mapping of intrachromosomal loci, a major benchmark in ...characterizing the 4D nucleome. Future modifications and applications Ma et al. developed this technique using standard...for this technique. The NIH Common Fund has made mapping the 4D nucleome a specific priority, as researchers...

  6. Editor's Choice, September 2016

    Type
    Blog Post
    ...exhaustive list of all the apps out there, we encourage you to mention any apps you’ve found useful in the... other posts that deserve a second look. 5 Great Apps for Lab Life This very useful post was contributed...provides a brief rundown of Sean’s top five smartphone apps you can use in the lab. Whether you’re tired of ...that chemical in your obscure growth medium, these apps can help make your lab life a bit easier. Since ...

  7. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...sure I missed some of your favorite apps. If you know of a software app that I missed, let me know in the... The software can be used with the ZappyLabs Bench Tools mobile app (available for iOS and Android). A...system does not appear to have a way to share your protocols with other groups. What apps do you use to ...publishers, and small start-ups are turning to software apps to solve our need for quicker, more effective updating...more to fit every lab's needs. Plus there are the apps - many to help you access your protocols at the ...comments below and leave your thoughts on how the app works for your lab’s needs. At Addgene, we’ll continue... some of our basic protocols to the protocols.io app so that all of our researchers can easily take those...

  8. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...their use in other clinical applications. In the last decade, viral applications in cancer treatment and ...s unpack the myriad number of emerging viral applications and give you some recommendations for putting...putting viruses to work. Viral applications Viruses work by infecting a host cell (the target of viral infection...expressed. It’s important to note that for all the applications we discuss, there is more than one type of virus...retrovirus, AAV, etc.). When you’ve decided on an application, our Viruses as Biological Tools blog can help...viruses and pick one for your experiment. Research applications Gene expression control Researchers are always...becoming increasingly popular tools with many applications. These libraries include sgRNAs targeting the...

  9. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...have been developed for different scientific applications. These PCR methods all use the same basic PCR...quantified by qPCR. qPCR is used in a variety of applications including gene expression profiling, studying...that fractions the PCR reaction sample into approximately 20,000 droplets. Each droplet contains the material...

  10. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...and applications.    Plant applications We outlined many synthetic biology MoClo applications, but...for synthetic biology applications in bacteria or cell-free systems. The approach is particularly useful...- and multi-gene constructs for a variety of applications. MoClo tools can be used in bacterial and cell-free...and the kits Addgene offers to kickstart these applications, no matter what your model organism is. MoClo...this blog, we will highlight some common MoClo applications and kits. Keep in mind that this technology ... it, MoClo might help you achieve it! MoClo applications and specialized tools Synthetic biology in bacteria...effectiveness. Perhaps the most intuitive CRISPR MoClo application is to assemble multiple unique sgRNAs into a ...

  11. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...function (Wood, et al). In the microbial world, approximately 50% of predicted genes have unidentified roles...endogenous gene. Each trap uses a slightly different approach (described in detail below), but fundamentally...redundant genes – multiple genes which function in overlapping roles can mask the depletion of one another. ...trap systems lack a promoter and thus require the trapping of an endogenous promoter. The system is composed...enhancer traps lack enhancer elements and require the trapping of endogenous enhancers to stimulate the expression...many more! Check out Addgene’s catalog to find trapping tools for your next experiment! References and...

  12. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...particular application can be found here.  Why CRISPRa? Although cDNA overexpression approaches may still... viral vectors, and high-throughput screening approaches. Here, we’ll highlight these recent advancements...advancements and offer commentary on their application to neuroscience research. What is CRISPR-based activation... a larger packaging capacity than AAV. Such an approach was described recently by the Day Lab, who developed...accessible to neuroscientists, not all neuroscience applications require such optimized tools. This is elegantly... developed a subgenome-scale CRISPRa screening approach in embryonic stem (ES) cells to determine which...study neuronal differentiation, it could also be applied to the differentiation of other CNS cell types,...

  14. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...options, you’re likely to find an application that matches your needs. Happy quantifying!  References and...blog post, we're going to focus on PCR-based applications (qPCR and ddPCR.) qPCR Quantitative/real-time...range of RQ values as noted above.   In both applications, results can be affected by DNA quality and ...as positive or negative for each reaction, math happens (this time, Poisson statistics), and the readout... triplicates. ddPCR is an extremely accurate application, even in areas of the genome that are difficult...experiments, a number of factors will determine which approach is right for your experiment. You’ll have to consider...QuantificationPolymerase Chain Reaction Overview and Applications Resources on Addgene.org Protocol: Polymerase...

  15. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...The Framework for In vivo T cell Screens (FITS) approach (Milling and Markson et al., 2024), developed ...compatible with single gene KO and pooled screening approaches; (4) encodes a Thy1.1 selectable marker to facilitate... et al., 2024.   Proof of concept The FITS approach was used to optimize conditions for a screen that...

  16. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...protein for your application. Selecting the best fluorescent protein for a specific application can seem like...under conditions that best mimic the intended application. The three key properties for live cell imaging...theoretical brightness. The theoretical brightness often appears in tables with fluorescent protein properties and...experimental set up. The practical brightness takes all application specific parameters into account, including the... picture of what can be expected in a 'real' application than the theoretical brightness. Consequently...brightness in a system closely mimicking the future application. In figure 2 we provide an example of the comparison...

  17. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...explore alternate approaches. Being a “lazy” geneticist, I developed a co-selection approach relying on repair...with unprecedented ease. These new C. elegans approaches rapidly enrich for editing events without the...phenotypes (unc-119) (Dickinson et al., 2013). These approaches allowed effective recovery of knock-ins, but ...Including Cre-mediated marker excision, it takes approximately 3-4 weeks to obtain a homozygous, outcrossed...several manuscripts published in Genetics detailed approaches in which selection for an editing event that ...and knock-ins at other genomic loci. The first approach – co-CRISPR from Craig Mello’s lab – used inactivation...animals with particular visible phenotypes. These approaches may become even more powerful following a report...

  18. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...cas9 gene and single guide RNAs (sgRNAs) with appropriate flanking homology into a germline cell can create...RNA-guided gene drive. In principle, this event could happen even if there isn't any homology available. Consequently...local biosafety officer should be familiar with appropriate measures. Barriers should be a component of all...eventual release should consider building an appropriate immunizing reversal drive at the same time to...species in question), why not use them whenever applicable?Against this, consider the cost of an accidental...An accidental release could delay real-world applications against scourges such as malaria and dengue .... Disclaimer: Recommendations concerning the appropriate degree of confinement are those of the author...

  19. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    ... the app. Technical difficulties? Attending a conference virtually means getting to know the app or virtual...conferences with multiple sessions happening at once, virtual conferences may have apps or interactive calendars...was able to get in touch with the author. “In the app, you can write a message to the author of the poster...there may be a “place” for networking within the app. For example, the NeuroSymposium had virtual tables... puts butterflies in your stomach, the virtual approach might be less stressful. For both conferences,...Having an on-site conference canceled may be disappointing, but there are many creative ways to adapt and...

  20. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...There are approximately 2-4 million proteins per cubic micron in bacteria, yeast, and mammalian cells...based affinity purification and mass spectrometry approaches. Recently however, scientists have developed ...miniturbo Split-BioID: A conditional proteomics approach to monitor protein-protein interactions Split-... PMC3308701. Rhee, Hyun-Woo, et al. "Proteomic mapping of mitochondria in living cells via spatially restricted..., et al. "Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally...proximity-based labeling methods for interactome mapping. F1000Research, 8.  ...

  21. Antibody Guide

    Type
    Collection
    ...single assay. Overview of Antibody Applications Antibody-based applications can be generally classed into ...these applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...of signaling molecules. Select one appropriate for your application and experimental design. Will you ...transcriptomics or proteomics. This approach is often appropriate for quantitative methods. Capture MS...protein of interest, with no overlapping epitopes. It is not appropriate for validation of an antibody...production and storage techniques, and explain common applications. Science... Handling Visualization Signal Amplification Application Overview Quantification Methods Capture Methods...

  22. Immunology Research Plasmids and Resources

    Type
    Collection
    ...binding protein 1 CREB, MGC9284 CTSB cathepsin B APPS, CPSB CTSE cathepsin E CATE CTSL1 cathepsin L1 CATL...immunoglobulin kappa joining 1 J1 IGKJ2 immunoglobulin kappa joining 2 J2 IGKJ3 immunoglobulin kappa joining ...immunoglobulin kappa joining 4 J4 IGKJ5 immunoglobulin kappa joining 5 J5 IGKV@ immunoglobulin kappa variable...IGKDEL immunoglobulin kappa deleting element or like IGKDE IGKJ@ immunoglobulin kappa joining group IGKJ ...IGKV1-12 immunoglobulin kappa variable 1-12 IGKV112, L19 IGKV1-13 immunoglobulin kappa variable 1-13 (gene...IGKV1-16 immunoglobulin kappa variable 1-16 IGKV116, L1 IGKV1-17 immunoglobulin kappa variable 1-17 A30, IGKV117...IGKV1-27 immunoglobulin kappa variable 1-27 A20, IGKV127 IGKV1-33 immunoglobulin kappa variable 1-33 IGKV133...

  23. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...sfGFP David Spray 54959 mApple-VE-Cadherin-N-10 Tight Junctions VE-Cadherin mApple Michael Davidson 55165...moxGFP Erik Snapp 68126 ERoxBFP Endoplasmic Reticulum ER retention signal oxBFP Erik Snapp 49150 BFP-KDEL...GolgiCFP Golgi apparatus eNOS(1-33) CFP Alexandra Newton 36205 pmTurquoise2-Golgi Golgi apparatus B4GALT1(1...68073 GalToxBFP Golgi apparatus GalT signal anchor transmembrane domain oxBFP Erik Snapp 158005 pCMV-mGold-MannII-N...10 Golgi apparatus MannII mGold Francois St-Pierre 85050 pmScarlet-i_Giantin_C1 Golgi apparatus Giantin...pmScarlet-H_Giantin_C1 Golgi apparatus Giantin mScarlet-H Dorus Gadella 85048 pmScarlet_Giantin_C1 Golgi apparatus Giantin ...Giantin Golgi apparatus Giantin mTurquoise2 Dorus Gadella 98880 mNeonGreen-Giantin Golgi apparatus Giantin ...

  24. CRISPR Guide

    Type
    Collection
    ... (2015). A scalable Genome-Editing-Based approach for mapping multiprotein complexes in human cells. Cell...technologies. Before CRISPR, genome engineering approaches like zinc finger nucleases (ZFNs) or transcription-activator-like...rapidly became the most popular genome engineering approach. Figure 1: Overview of the basic CRISPR mechanism... to various Cas enzymes have extended CRISPR applications to increasingly complex functions, including...understanding of CRISPR biology, introduce the various applications of CRISPR, and help you get started using CRISPR... Cas12a, which are available for specialized applications. Check out our list of additional Cas proteins...genomic edits carried out by Cas9. Multiplexing applications include editing multiple genes at once; using...

  25. Plan Your Experiment

    Type
    Collection
    ...Cas9 in mammalian cells, many of these principles apply to using CRISPR in other organisms. First, consider...a good way to narrow down which reagents are appropriate for a given experiment. Make sure to check whether...may not be a perfect plasmid for your specific application, and in such cases, it may be necessary to customize...reagent to suit your needs. Genetic Manipulation Application Cas9 gRNA Additional Considerations Knockout ...Cas enzymes increase specificity. Dual-nickase approach increases specificity but is less efficient. Each...viral delivery of CRISPR reagents may be more appropriate. In cases where off-target editing is a major...method Expression System Components of System Application Mammalian expression vector Cas enzyme promoter...

  26. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...therapeutic applications. Despite the ethical controversies surrounding non-research applications, it’s clear...Y. 2015. A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell...just as useful (if not more so) for research applications, eclipsing past genome engineering technologies...proteins snip foreign DNA into small fragments approximately 20 bp in length and paste them into contiguous...for CRISPR genomic editing. Genome Engineering Applications In 2012, Jinek et al. first demonstrated that...various species, as well as the development of new applications. The first CRISPR papers described two main ...RNA, making it a very flexible system. Other Applications Scientists have also used the targeting capability...

  27. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Bacterial Expression mApple 568 592 37 6.5 30 min Monomer mApple-N1 - Mammalian Expression mApple-C1 - Mammalian...Expression mT-sapphire 399 511 Monomer (A206K) mT-Sapphire-N1 - Mammalian Expression mT-Sapphire-C1 - Mammalian...Structure Plasmids T-sapphire 399 511 26 4.9 1.3 hr Prone to dimerization pFA6a-link-yETSapphire-CaUra3 - Yeast...for this page was generated with the help of Erik Snapp . Empty Backbones for Fluorescent Protein Fusions...Mammalian Expression mApple-pBAD - Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-...Mammalian Expression mT-Sapphire-pBAD - Bacterial Expression mAmetrine 406 526 26 6 0.8 hr Monomer (A206K...

  28. AAV Packaged on Request

    Type
    Collection
    ...production will take approximately 6–8 weeks. Additional steps, including request approval, your institution...requests will be approved within two business days! Ordering At your convenience After approval, the next step...facilitation for the transfer plasmid, including legal approval and an implementation letter DNA amplification...institution’s approval of the MTA, and shipping, mean that the actual time from request to delivery could be up...before we can begin processing your order. MTA Approval 1+ business days, varies by requesting organization...remain subject to the terms and conditions of the applicable transfer plasmid. We will help you gain legal...

  29. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ...for your needs 5 great apps for lab life  Sharing your lab protocols using apps to save you time and track...sign-in required.)  DailyCalcs Science Calculator app for iPhone Reagent and Data Repositories These links...paste into other applications Primer design tools Many of the above-listed plasmid mapping tools have built-in...Primer design. Plasmid mapping. DNA sequence analysis. We all have our favorite tools for tackling these...molecular biology tools all in one place. Plasmid mapping These tools are for viewing, editing or making ...the paid version provides more tools for plasmid mapping and design (Figure 1). Benchling: While you might... desktop-based software for plasmid design and mapping. ApE (A plasmid Editor): A free, donation-based...

  30. All Antibodies

    Type
    Collection
    ...antibodies. These monoclonal antibodies undergo application-specific validation and quality control by Addgene...antigen. Addgene supplies a list of recommended applications based on our in-house testing and data provided...with full experimental details in the Antibody Applications section of our product pages. We also include... an antibody’s use is not recommended for an application or species. We currently assess western blot,...scientists to further develop and refine these application lists. The plasmids we use to produce antibodies...Source Species Isotype Reactivity Recommended Applications PI Return to Top Do you have suggestions for...

  31. Deisseroth INTRSECT Collection

    Type
    Collection
    ...proof-of-concept targeting approach in 2014 1 (using EYFP and ChR2-EYFP as payloads). This approach has been broadly...gene or projection). Intersectional expression approaches combine multiplexed recombinases, including Cre...broadly applied using multiple recombinase-expression strategies to define cellular sub-populations of interest... Oberhuber M, Conzelmann KK, Staiger JF. 2019. Mapping Brain-Wide Afferent Inputs of Parvalbumin-Expressing... Dombeck DA, Deisseroth K, Awatramani R. 2018. Mapping projections of molecularly defined dopamine neuron...neuron subtypes using intersectional genetic approaches. Nat Neurosci. 21(9):1260-1271. PubMed (Link opens...

  33. Antibody Plasmid Collection

    Type
    Collection
    ...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene...generate due to expense and time. An alternative approach is to use synthetically produced recombinant antibodies...extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant mAb...imaging. Learn more about antibodies and their applications in our Antibody Guide ! Cloning and Expressing...single domain antibodies for the conformational trapping of membrane proteins. Elife. 2018 May 24;7. ...

  34. Tetracycline Inducible Expression

    Type
    Collection
    ...tTA-Advanced Takeshi Imai Other Tet Applications Explore some other popular applications of tetracycline systems ... of interest from one plasmid. A single vector approach may not have as much flexibility and control as...for comparison of Tet-On systems in different applications. Use of Tetracycline or a Derivative Doxycycline...vectors for transactivators, and vectors for other applications, or search our collection for all dox-regulated...154939 dCas13d-dsRBD-APEX2 RNA-protein interaction mapping via Cas13-based APEX targeting Alice Ting 104102...

  35. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...robotic multidimensional directed evolution approach applied to fluorescent voltage reporters. Nat Chem...sensor's behavior in the target system. Use care when applying a biosensor in a novel context or modifying an...indicators based on mRuby (jRCaMP1a, jRCaMP1b) and mApple (jRGECO1a) (Constitutive or Cre-dependent) Sensitive... Red fluorescent protein-based cAMP indicator applicable to optogenetics and in vivo imaging. Sci Rep....NADP+ ratio or NAD+ Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine...Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range. Biochemistry...Positron (Constitutive or Cre-dependent) A general approach to engineer positive-going eFRET voltage indicators...

  36. Antibodies

    Type
    Collection
    ...expressed from plasmids. These antibodies undergo application-specific validation and consistent quality control...and provide experimental data for recommended applications. Addgene values transparency and accessibility...antibodies developed and validated for many research applications. Browse Antibodies Production Interested in ... storage techniques, and explain common applications. View Guide Need Some Help? Browse some of our...

  37. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...utility for the study of non-coding elements. This approach can be used for efficient loss-of-function studies...example, sgRNA-A’s protospacer sequence and PAM happen to fall on the bottom (Crick) strand while sgRNA-B... the presence or absence of deletion. For this approach, the internal primers described in step 2.1 may...Phosphorylated oligos may be ordered instead. For this approach, the use of T4 PNK is omitted. Anneal in a thermocycler... to performing this step to reliably obtain approximately 30 cells per 96-well plate. Include 100 μl per...

  38. Bacterial Expression Systems

    Type
    Collection
    ...sequence? Do these two proteins interact? What happens when I overexpress this enzyme at a particular ...cellular activity in a way that’s interesting from an applied perspective, sometimes it’s best to delete genes...bacteria of interest are transformed with the appropriate construct, grown at the permissive temperature...localization of your gene of interest once its appropriate cDNA has been fused to the MS2 binding sites ...

  39. Rett Syndrome

    Type
    Collection
    ...females at an incidence of approximately 1:10,000. It is characterized by apparently normal development through...In females, random X inactivation results in approximately half of cells expressing wild-type MECP2 and...Tools CRISPR technology is a promising therapeutic approach for precise editing of mutations in the MECP2 ...

  40. Luciferase Plasmid Collection

    Type
    Collection
    ...advantages and disadvantages depending on the application. Firefly luciferase is considerably brighter ...luciferase-based plasmid systems for many different applications. Browse the Collection Highlights section to...respectively, for increased usefulness in in vivo applications. LuxSit-i : An artificial luciferase desined...desined de novo using deep-learning approaches. Split-Luciferase and Protein-Protein Interaction Assays Cluc-FKBP...

  41. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...insertions — meaning they may not be suitable for applications where orientation of the insert is important... TnsC, and TniQ). Unlike type I, type V-K CASTs appear to exhibit almost unidirectional insertions, but...

  42. Institute for Protein Innovation

    Type
    Collection
    ...Antibody Description Isotype Reactivity Recommended Applications Integrin Antibodies Integrins are a family of...Antibody Description Isotype Reactivity Recommended Applications Browse Epitope Tag Antibodies IPI has developed...Antibody Description Isotype Reactivity Recommended Applications Browse Antibody Plasmids The following plasmids...

  43. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...subject to Addgene’s Terms of Purchase and the applicable material transfer agreements (MTAs) and limited...generally ready for shipment within 8 weeks after the approval of the MTA by your institution. Our final step...contact you directly regarding these charges, if applicable....

  44. Distribution to Industry

    Type
    Collection
    ...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene... Recombinant Antibody Reactivity Recommended Applications PI Don’t See What You’re Looking For? Please...email to [email protected] . After we review and approve the MTA documents, your order will be processed...

  45. AAVED

    Type
    Collection
    ...useful to help improve this technology or its application? What are some current obstacles that researchers...antibodies and recommended dilutions, etc.), novel applications, or any other useful information. Experimental...take care of that. Note that you will have final approval before anything is released publicly, but please...

  46. Microbiology Resources

    Type
    Collection
    ...our Search page to look for specific genes or applications that are not listed here. Addgene Microbiology...Densmore Lab EcoFlex MoClo : Modular cloning for applications like recombinant protein purification and cell-free...fluorescent protein plasmid collection is organized by application and by color. External Resources European Saccharomyces...

  47. TALEN Guide

    Type
    Collection
    ...your dissertation. Well, that will probably never happen. Yet with transcription activator-like (TAL) effectors... they still have potential targetable sites approximately every 35bp. Researchers are still determining...arrays from 12-31 repeats. Perhaps one of the most appealing features of the TAL effector arrays is the ease...

  48. Brain Initiative Collection

    Type
    Collection
    ... the human brain through the development and application of innovative tools enabling large-scale real-time.... Useful for nuclear isolation and scRNA-seq applications. Jonathan Ting 163909-AAV9 pAAV_hSynapsin_psychLight2...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene... Recombinant Antibody Reactivity Recommended Applications PI Additional Resources To learn more, please...

  49. COVID-19 Resources

    Type
    Collection
    ... antibody escape mutants and for comprehensive mapping. Some of the following plasmids are available to... Spike protein RBD mutations for comprehensive mapping (Jesse Bloom). SARS-CoV-2 Spike Receptor Binding... Spike protein RBD mutations for comprehensive mapping (Jesse Bloom, Tyler Starr). External Resources ...

  50. Zhang Lab CRISPR Page

    Type
    Collection
    ... nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single guide...function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo , the Zhang Lab established...can be combined with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin...
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