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We narrowed to 430 results for: ERG

Showing: 1 - 20 of 430 results

  1. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    ...viral service items we employ dozens of pieces of energy-intensive lab equipment on a daily basis. By the...allows a community like Watertown to bulk purchase energy from a higher percentage of renewable sources. ... drawing 100% of our electricity from renewable energy projects right here in New England- the majority...ventilation, and lab lighting/HVAC requires a significant energy expenditure here at Addgene. These are critical... or substantially reduced in an attempt to save energy. The transition to 100% renewable electricity will... will allow us to maintain these particularly energy-intensive systems in a more environmentally responsible...

  2. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...scientists I’ve met began doing research in their undergraduate years. We each have a unique “origin story,”... provide some perspective and advice to any undergraduate interested in getting a taste of the role of...Professor Sometimes teaches graduate and/or undergraduate classes Postdoctoral researcher (postdoc...choosing one as their official thesis lab Undergraduate student Roles and responsibilities can vary...would also highly recommend talking to other undergraduate researchers, as well as graduate students (your...’s something you want to dedicate your time and energy to. How can I make the most of my experience in...valuable for anyone in the lab though, including undergrads. I found a lot of career resources on Science...

  3. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ... in their careers? A CURE for Biochemistry Undergraduate biochemistry courses are often associated with... what is now commonly termed a Course-based Undergraduate Research Experience (CURE). Having incorporated...Knutson et al., 2010a,b).      Fig. 1: Undergraduates in Provost’s CURE prepare to analyze their ...original research experiences for thousands of undergraduates. The MCC develops and makes freely available...production.   CUREs are beginning to transform undergraduate education in the sciences by making a greater... State College in New Hampshire. He mentors undergraduates conducting NIH- and NSF-funded research on ...Bangera, G., Brownell, S. 2014. Course-based undergraduate research experiences can make scientific research...

  4. Enabling high school research at the Journal of Emerging Investigators

    Type
    Blog Post
    ...Addgene Podcast, we introduce you to the Journal of Emerging Investigators, an open-access journal that enables...Addgene. Listen to the interview with the Journal of Emerging Investigators Podcast breakdown Intro Chapter ... Springel, Olivia Ho-Shing, and the Journal of Emerging Investigators. Chapter 2 - Getting high school...

  5. Pipetting Protocol

    Type
    Protocol
    ... lower the pipette tip into the liquid. Do not submerge the pipette itself into the liquid and avoid touching...contaminating the contents. Once the end of the tip is submerged, slowly release the plunger, allowing it to go...

  6. Pouring LB Agar Plates

    Type
    Protocol
    ... a water bath at 60 ℃ with sufficient water to submerge ~75% of the bottle containing your molten gel ...removing anything from the autoclave. Partially submerge your molten gel-mix in the 60 ℃ water bath. Pro-Tip...

  7. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...dvpr and envelope plasmid pCMV-VSVG from Robert Weinberg’s lab. For more information, visit Addgene’s Mammalian... pCMV-dR8.2 dvpr and pCMV-VSVG from the Robert Weinberg lab. For more information, visit Addgene’s Mammalian...

  11. AAV Production in HEK293 Cells

    Type
    Protocol
    ...cells is critical for optimal AAV yield. Do not overgrow your cells. Pass the cells twice a week during...

  12. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Example dilutions and titrations Reference Wang Y, Bergelson S, Feschenko M. Determination of Lentiviral Infectious...

  13. Brain Initiative Collection

    Type
    Collection
    ...-dependent dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer...recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer...

  14. CRISPR Guide

    Type
    Collection
    ...cleavage. Generating a Knockout Using CRISPR Cas9 undergoes a second conformational change upon target binding...stability PEmax - optimized PE architecture; increased synergy between PE enzymes and epegRNAs for enhanced editing... ssisted T argeting), developed by Samuel H. Sternberg’s lab . Many of these systems are based on naturally...argeting E lements), developed by the Abudayyeh - Gootenberg lab , also builds off prime editing. PASTE uses...Welch, M. M., Sousa, A. A., Harrington, L. B., Sternberg, S. H., Joung, J. K., Yildiz, A., & Doudna, J.... S., Okazaki, S., Noda, T., Abudayyeh, O. O., Gootenberg, J. S., Mori, H., Oura, S., Holmes, B., Tanaka...Ran, F. A., Cong, L., Yan, W. X., Scott, D. A., Gootenberg, J. S., Kriz, A. J., Zetsche, B., Shalem, O.,...

  15. Plasmids for Stem Cell Research

    Type
    Collection
    ...Fibroblasts Dopaminergic Neurons Lentiviral Human Direct conversion of human fibroblasts to dopaminergic neurons...fibroblasts into cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fetal Lung Fibroblasts Cholinergic Neurons...Biotechnology. 2014 Dec;32(12):1223-30. Zhou Astrocytes Dopaminergic Neurons Lentiviral Human Efficient conversion...conversion of astrocytes to functional midbrain dopaminergic neurons using a single polycistronic vector. PLoS...10(4):465-72. Schöler Adult Skin Fibroblasts Cholinergic Neurons Lentiviral Human Small molecules enable...to efficiently convert human fibroblasts into cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fibroblasts...neurons. Unpublished. Ward Glial Progenitor Cells GABAergic Interneurons Lentiviral Human Direct Conversion...

  16. Brain Armamentarium

    Type
    Collection
    ...CN5140) Expression of CoChR-EGFP in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru 214862...plus separate nuclear FLAG epitope in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru 214857...) Expression of ChR2(H134R)-EYFP in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru 213945... mCherry-tagged channelrhodopsin-2 driven by cholinergic neuron-targeting enhancer Gordon Fishell Viviana...dTomato_nlsdTomato AAV construct expressing dTomato driven by cholinergic neuron-targeting enhancer Gordon Fishell Viviana...fluorescent protein) in striatal and lateral septum cholinergic neurons Jonathan Ting Viviana Gradinaru 191708...BGHpA AAV vector for Cre expression in forebrain GABAergic interneurons. For use in combination with Cre-...

  17. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Targeted sensors for glutamatergic neurotransmission Targeted sensors for glutamatergic neurotransmission...fluorescent protein (cpFP) or Förster resonance energy transfer (FRET) efficiency of a pair of fluorescent...Cohen Calcium GCaMP6f expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer...Griesbeck Lab Twitch Plasmids Oliver Griesbeck Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca+ dynamics...Fluorescent sensor of ATP:ADP ratio (PercevalHR) Imaging energy status in live cells with a fluorescent biosensor...and metabolism (FLIPmal) Fluorescence Resonance Energy Transfer sensors for quantitative monitoring of... acetylcholine sensor for monitoring in vivo cholinergic activity (GACh3.0) (Constitutive or Cre-dependent...

  18. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...minBG-SYFP2-WPRE3-BGHpA AiP11390 DLX2.0 SYFP2 Pan GABAergic interneurons Isocortex 222939 pAAV-DLX2.0-minBG-FlpO-WPRE-HGHpA...minBG-FlpO-WPRE-HGHpA AiP14532 DLX2.0 FlpO Pan GABAergic interneurons Isocortex 214518 pAAV-AiE2366m-minBG-SYFP2...minBG-SYFP2-WPRE3-BGHpA AiP13038 AiE0743m_3xC2 SYFP2 Cholinergic interneurons Striatum 214848 pAAV-AiE0743m_3xC2...minBG-iCre(R297T)-BGHpA AiP13780 AiE0743m_3xC2 Cre Cholinergic interneurons Striatum 214858 pAAV-AiE0873m_3xC2...10aa-H2B-WPRE3-BGHpA AiP14496 AiE0873m_3xC2 SYFP2 Cholinergic interneurons Striatum 214868 pAAV-AiE0873m_3xC2...minBG-iCre(R297T)-BGHpA AiP15138 AiE0873m_3xC2 Cre Cholinergic interneurons Striatum 191706 pAAV-AiE0779m_3xC2...Gwinn RP, Cobbs C, Ko AL, Ojemann JG, Keene CD, Silbergeld DL, Sunkin SM, Gradinaru V, Horwitz GD, Zeng ...

  19. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...watermelon mutant clones for your course-based undergraduate research experience (CURE) projects....Community (MCC) Collection Blog post: Course-Based Undergraduate Research Experiences (CUREs) (Link opens in ...window) develops protein-centric Course-based Undergraduate Research Experiences (CUREs) centered on a single...opens in a new window) Length of course-based undergraduate research experiences (CURE) impacts student ...Increasing access for biochemistry research in undergraduate education: The malate dehydrogenase CURE community...

  20. Retrovirus Plasmids

    Type
    Collection
    ... immortalized cells (see Weinberg Lab page for resistance variants) Weinberg 1773 pBABE-hygro-hTERT MoMLV...Contains MoMLV gag and pol; use with envelope plasmid Weinberg 12371 pCL-Eco Packaging Contains MoMLV gag, pol...Envelope Envelope protein; use with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope...plasmids include Puro resistance and Neo resistance Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA...
Showing: 1 - 20 of 430 results