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We narrowed to 306 results for: SPI

Showing: 1 - 20 of 306 results
  1. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    ...SARS-CoV-2 spike variants. “We created a series of spike expressing plasmids which encode spikes found on... Figure 1: Neutralization levels against spike pseudovirus decreases depending on the variant. ...Garcia-Beltran et al., 2021 with permission.   The spike variant pseudovirus neutralization assay Balazs’..., 2021 with permission.   Mutations in the spike protein are one of the key drivers in determining...
  2. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...Huber S, Siegrist M, Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER, Dawson RJ, Seeger MA (2018... identified nanobodies that bind to coronavirus spike proteins (Wrapp et al., 2020), which sit on the ...enter host cells. To do this, they injected the spike protein from two different coronaviruses into a ...isolated special camelid antibodies that bind to the spike proteins in cell culture, and inhibit these viruses...generate sybodies, synthetic nanobodies against the spike protein (Walter et al., 2020). No llamas required...Receptor Binding Domain found on the membrane-bound spike protein, in a remarkable 12 working days. While ...
  3. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...PRKCG MTH Spinocerebellar ataxia 30 Frederic Mushinski 8418 pLTR PKC gamma PRKCG Spinocerebellar ataxia ...GFP CMV Spinocerebellar ataxia 27 Tobias Meyer 21204 GFP-N2-PKCgamma PRKCG GFP CMV Spinocerebellar ataxia...GFP CMV Spinocerebellar ataxia 25 Tobias Meyer 21215 GFP-C2gamma PRKCG GFP CMV Spinocerebellar ataxia ...CMV Spinocerebellar ataxia 23 Bernard Weinstein 21237 PKC gamma dNPS PRKCG HA CMV Spinocerebellar ataxia... CMV Spinocerebellar ataxia 21 Bernard Weinstein 21239 PKC gamma DN PRKCG HA CMV Spinocerebellar ataxia...CMV Spinocerebellar ataxia 15 Ryohei Yasuda 112270 CMV-PKCg-mEGFP-N1 PRKCG GFP CMV Spinocerebellar ataxia...GFP CMV Spinocerebellar Ataxia Richard Youle 176464 pVPS13D-I-EGFP VPS13D GFP CMV Spinocerebellar Ataxia...
  4. COVID-19 Resources

    Type
    Collection
    ...all spike expression plasmids in our collection that are suitable for generating SARS-CoV-2 spike ...SARS-CoV-2 Spike (S) Ectodomain and RBD Libraries Screening Timothy Whitehead Libraries of Spike (S) Ectodomain...Bloom Library of Spike protein RBD mutations for comprehensive mapping. SARS-CoV-2 Spike Receptor Binding...entry driven by the severe acute respiratory syndrome coronavirus spike protein . Journal of virology, ...SARS-CoV and MERS CoV, depends on binding of the viral spike (S) protein to cellular receptors. The cellular ...expressing genes encoding SARS-CoV-2 proteins, (spike protein, S; small envelope protein, E; nucleocapsid... E. coli , yeast, and mammalian expression, and spike protein constructs for making...
  5. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...Iterative Synthetically Phosphorylated Isomers (iSPI) The iSPI resource described in Galloway et al. (2022)...Pooled Library 188536 iSPI_pSer_Full_library Pooled Library 188535 iSPI_pSer_Subpool#10 Pooled Library 188534... 188534 iSPI_pSer_Subpool#9 Pooled Library 188533 iSPI_pSer_Subpool#8 Pooled Library 188532 iSPI_pSer_...Pooled Library 188531 iSPI_pSer_Subpool#6 Pooled Library 188530 iSPI_pSer_Subpool#5 Pooled Library 188529...188529 iSPI_pSer_Subpool#4 Pooled Library 188528 iSPI_pSer_Subpool#3 Pooled Library 188527 iSPI_pSer_Subpool...using iSPI, rEcoli XPS, and pSerOTS-C1* (V70) (ca. 2022) from the Rinehart lab: Methods for iSPI, rEcoli...phosphoprotein expression in rEcoli XpS in the Methods for iSPI, rEcoli XPS, pSerOTS-C1* (V70) (DOCX, 551 KB) . ...
  6. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...Plasmids Expressing Spike and Spike Variants Full-length wildtype SARS-CoV-2 spike protein is inefficient...Browse Addgene's COVID-19 collection of SARS-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids...plasmids for viral pseudotyping with SARS-CoV-2 spike protein. Collections...Pseudotyping SARS-CoV-2 Pseudotyped Virus SARS-CoV-2 Spike Plasmids Accessory Plasmids Other Resources Mechanisms...replication-defective virus particles with the SARS-CoV-2 spike (S) glycoprotein on the envelope surface. Pseudotyped...particles have been pseudotyped with the SARS-CoV-2 Spike protein and you will find examples of each in Addgene's.... Use the search bar to find a particular viral spike protein (MERS, CoV-1, CoV-2), modification, or variant...
  7. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...-BGHpA AiP13044 AiE0779m_3xC2 SYFP2 DRD1 Medium spiny neurons (D1-MSN) Striatum 199776 pAAV-AiE0779m_3xC2...R297T)-BGHpA AiP13779 AiE0779m_3xC2 Cre DRD1 Medium spiny neurons (D1-MSN) Striatum 214855 pAAV-AiE0779m_3xC2...minBG-FlpO-WPRE3-BGHpA AiP14201 AiE0779m_3xC2 FlpO DRD1 Medium spiny neurons (D1-MSN) Striatum 224206 pAAV-AiE0780m-minCMV-SYFP2...WPRE3-BGHpA AiP15827 AiE0780m SYFP2 DRD1 Medium spiny neurons (D1-MSN) Striatum 224200 pAAV-AiE0780m-minBG-iCre...(R297T)-BGHpA AiP15578 AiE0780m Cre DRD1 Medium spiny neurons (D1-MSN) Striatum 191707 pAAV-AiE0452h-minBG-SYFP2...WPRE3-BGHpA AiP12237 AiE0452h SYFP2 DRD2 Medium spiny neurons (D2-MSN) Striatum 199777 pAAV-AiE0452h-minBG-iCre...(R297T)-BGHpA AiP13781 AiE0452h Cre DRD2 Medium spiny neurons (D2-MSN) Striatum 230871 pAAV-AiE0452h-minBG-FlpO-WPRE3...
  8. Brain Armamentarium

    Type
    Collection
    ...CN4825) Expression of iCre(R297T) in striatal medium spiny neurons Jonathan Ting Viviana Gradinaru 214858-PHPeB...of jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan Ting Viviana Gradinaru...Expression of jGCaMP8s in striatal direct pathway medium spiny neurons (D1 MSNs) Jonathan Ting Viviana Gradinaru... CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan Ting Viviana Gradinaru...of CoChR-EGFP in striatal direct pathway medium spiny neurons (D1 MSNs) Jonathan Ting Viviana Gradinaru...of jRGECO1a in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan Ting Viviana Gradinaru...for Cre recombinase expression in striatal medium spiny neurons Jonathan Ting Viviana Gradinaru 199774-PHPeB...
  9. Genetic Code Expansion

    Type
    Collection
    ... synthetase M. mazei Mammalian TAG Ivana Nikić-Spiegel 182537 pEVOL-MmAcKRS1-PylTUUA PylRS M. mazei Bacterial...NESPylRS(AF) PylRS M. mazei Mammalian TAG Ivana Nikić-Spiegel 182653 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_... synthetase M. mazei Mammalian TAG Ivana Nikić-Spiegel 182881 Mut1-RS tyrosyl-tRNA synthetase M. jannaschii...
  10. Neurodegeneration Research Collection

    Type
    Collection
    ... upper and lower motor neurons in the brain and spinal cord. Over time, a loss of motor neurons leads ...brain. The cause of this neuron death is unknown. Despite the idiopathic (having no specific known cause)...
  11. Plasmids for Stem Cell Research

    Type
    Collection
    ... of mouse and human fibroblasts into functional spinal motor neurons. Cell Stem Cell. 2011 Sep 2;9(3):...2013 May;31(5):426-33. Tesar Fibroblasts Medium Spiny Neurons Lentiviral Human Generation of Human Striatal...
  12. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Serotonin (5-HT) sensor psychLight Psychedelic-inspired drug discovery using an engineered biosensor. ...glutamate receptor Glutamate receptor exocytosis and spine enlargement during chemically induced long-term ...
  13. Recombinases AAV Preps

    Type
    Collection
    ...pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE EF1a EGFP 8 Ivo Spiegel 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP none 5, PHPeB...
  14. p53 Pathway

    Type
    Collection
    ...growth factor binding protein 3 KAI CD82 molecule Maspin Serpin family B member 5 MDM2 MDM2 proto-oncogene...
  15. TALEN Guide

    Type
    Collection
    ...Dr. Keith Joung’s lab at Massachusetts General Hospital also recently released a TALEN kit through Addgene...
  16. AAV Molecular Tools

    Type
    Collection
    ...and mScarlet-KASH for silencing neurons. 8 Ivo Spiegel 200777 AAV-HAPLN1 hSyn-driven, constitutive Expresses...
  17. CRISPR References and Information

    Type
    Collection
    ...in Japan. Developed by the Kumiko Ui-Tei Lab . CRISPick (Link opens in a new window) Ranks and picks candidate...
Showing: 1 - 20 of 306 results