We narrowed to 202 results for: cycs

... particle — and of the normal, replicative life cycle — can help you understand these seemingly odd choices...vectors. Key to the wild-type virus’ reproductive cycle is the fact that the virus must have its structural...as the viral particle emerges. Retrovirus life cycle Retroviruses go against the grain of the Central...will discuss several aspects of the viral “life cycle” in order to understand what these vector particles... cells (Fig. 3).     Figure 3: The life cycle of a retrovirus. Image courtesy of Jeremy Luban,...Luban, MD. Figure 3 depicts the viral life cycle. One should ponder this figure often, especially the ...particles. And there you have it, the full “life cycle” of expression of selfish, lazy genes of a non-living...

... or city recycling services don’t offer plastic recycling, specialized companies can recycle plastic--...and to print a free shipping label. Recycle nitrile gloves Terracycle is a company that takes items not ... blog posts. This first post will be based on recycling! With the popularity of single-use items, plastic... generation. Less than 9% of plastics are ever recycled, so adopting a few meaningful changes in the lab...environmentally friendly. Adopt one or all of the recycling practices below. Reuse lab cooler boxes Some vendors...sticker off the box, remove the ice pack (it’s not recyclable), tape the box shut, and drop off the box at ... United States Postal Service picks up mail. Recycle plastic packaging Packaging is a huge contributor...

...Collections Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Tetracycline Off ...applications. Use of Tetracycline or a Derivative Doxycycline, a derivative of tetracycline, is a preferred...one of its analogs like doxycycline (dox) is present, TetR binds to tetracycline and releases tet O, enabling...shown. Tetracycline Off (Tet-Off) The first major advance was the Tet-Off system. A tetracycline-controlled...presence of tetracycline, tTA unbinds from the TRE, resulting in reduced gene expression. Tetracycline Off is...binds the TRE in the presence of tetracycline. A new reverse tetracycline-controlled transactivator (rtTA... absence of tetracycline, rtTA is unable to bind the TRE. In the presence of tetracycline, rtTA binds ...

...uracil-excision based (USER) sites, flanked by ADH1 and CYC terminators for easy cloning of genes and promoters...unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many of which ...control of the TRE promoter, which allows for doxycycline-inducible expression. The end result is genomic...controlled expression of the dCas9-VPR transgene by doxycycline in your target cells. This plasmid adds to the...gondii infection, and necessary for the asexual cycle of malaria parasites. Find Lourido CRISPR Plasmids...

... peptidase Cyclin B CCNB1 CCNB2 CCNB3 Cyclin B1, 2, or 3 Cyclin D CCND1 CCND2 CCND3 Cyclin D1, 2, or 3... 3 Cyclin E CCNE1 CCNE2 Cyclin E1 or E2 Cyclin G CCNG1 CCNG2 Cyclin G1 or G2 CDC25 Cell division cycle...also known as PMAIP1 p14ARF Cyclin-dependent kinase inhibitor 2A p21 Cyclin-dependent kinase inhibitor ...best-characterized effects of p53 are in promoting cell cycle arrest, apoptosis, or senescence in damaged cells...cycle 25C Cdk4/6 CDK4 CDK6 Cyclin-dependent kinase 4 or 6 CHK1 Checkpoint kinase 1 CHK2 Checkpoint kinase...

...:7351. Tetsuya Kitaguchi cAMP (cyclic AMP) Fluorescent sensor of cyclic AMP (cAMPr) for mammalian or insect...sensor for cyclic AMP. Sci Signal. 2018 Mar 6;11(520). pii: 11/520/eaah3738. Justin Blau cAMP (cyclic AMP) ...:4003-4012.e6. Xavier Nicol cyclic di-GMP FRET-based biosensor for cyclic-di-GMP measurement Asymmetrical...ratio. Nat Commun. 2013;4:2550. Gary Yellen cAMP (cyclic AMP) Yellow fluorescent cAMP indicator (Flamindo2...2014 Jun 24;9(6):e100252. Tetsuya Kitaguchi cAMP (cyclic AMP) Red fluorescent protein-based cAMP indicator... 2022 Nov;19(11):1461-1471. Haining Zhong cAMP (cyclic AMP) RAB-ICUE red biosensor for monitoring cAMP...pii: 10.1038/s41556-018-0200-6. Jin Zhang cAMP (cyclic AMP) Yellow-emitting homoFRET/anisotropy ICUE cAMP...

...C cycling 4-base Bsal-HFv2 37-16 °C cycling 4-base BsmBI-v2 42-16 °C cycling 4...enzymes and cycling conditions (see Table 1).   Enzyme Temperature Incubation time/cycling Overhang...MM 42-16 °C cycling 4-base BspQI, 1x NEBridge Ligase MM 42-16 °C cycling 3-base  PaqCl...Buffer 37-16 °C cycling 4-base PaqCl, 1x NEBridge Ligase MM 37-16 °C cycling 4-base Sapl...              37-16 °C cycling 3-base Sapl, 1x NEBridge Ligase MM 37-16 °C cycling 3-base Table...set with the Type IIS restriction enzyme and thermocycler protocol. It will also identify overhangs with... Bbsl-HF®, 1x NEBridge® Ligase MM 37-16 °C cycling 4-base Bsal-HFv2® 37 °C Static 4-base...

...Expression Species PI 44249 pdCas9-bacteria pTetO Anhydrotetracycline (aTc) Escherichia coli Stanley Qi 11518 pDest...coli Andreas Moeglich 68940 pRMC2 Pxyl/TetO Anhydrotetracycline (aTc) Staphylococcus aureus Tim Foster 36267...glutamicum Timothy Lu 17972 pSE100 Pmyc1/TetO Anhydrotetracycline (aTc) Escherichia coli , Mycobacterium tuberculosis...tuberculosis Sabine Ehrt 44561 pST-KT Pmyc1/TetO Anhydrotetracycline (aTc) Escherichia coli , Mycobacterium tuberculosis...extorquens Christopher Marx 44448 pLC291 pR/TetO Anhydrotetracycline (aTc) Methylobacterium extorquens Christopher...baumannii Jason Peters 127088 pMS17 tcp830 Anhydrotetracycline (aTc) Streptomyces sp. Maggie Smith 74065...Controlled Expression Resources Check out our Tetracycline (Tet) Inducible Expression Collection for an...

...cell cycle) Centrin-2 EGFP Erich Nigg 41151 pEGFP Cep170 C-term Centrioles (dependent on cell cycle) Cep170...Endosomes (recycling) Rab11a TagRFP James Johnson 79805 pTag-BFP-C-h-Rab11a-c-Myc Endosomes (recycling) Rab11a...Endosomes (recycling) Rab4a TagRFP James Johnson 79799 pTag-BFP-C-h-Rab4a-c-Myc Endosomes (recycling) Rab4a...Endosomes (recycling) Rab11a EGFP Richard Pagano 12679 DsRed-rab11 WT Endosomes (recycling) Rab11a DsRed...41163 pEGFP PICH Centromeres (dependent on cell cycle) PICH EGFP Erich Nigg 49149 mCh-alpha-Tubulin Microtubules...

...cell cycle length by examining the ratio of blue to red cells. Cells undergoing rapid cell cycles have...C. elegans Fluorescent timers for studying cell cycle length The CRISPR corner New from the viral service....org/10.1101/2020.07.02.185249 Predicting cell cycle length with a histone-fused fluorescent timer By...By Kate Harten DeMaio Studies of the cell cycle attempt to understand development, regeneration, and certain...states. However, it’s difficult to observe the cell cycle in vivo, particularly if the cells are migratory...blue to red ratio, whereas cells with long or slow cycles are more red than blue (Eastman et al., 2020). ...expression of H2B-FT consistently predicted the cell cycle speed across these different cell types. In one ...

...;318(5858):1917-20. Thomson Lentivirus Human Doxycycline-inducible expression of human Oct4, Sox2, Klf4...Cibelli Lentivirus Human Single polycistronic, doxycycline-inducible lentiviral vector for the expression...322(5903):945-9. Hochedlinger Lentivirus Mouse Doxycycline-inducible expression of mouse Oct4, Sox2, Klf4...-9. Jaenisch Lentivirus Mouse Polycistronic, doxycycline-inducible lentiviral vectors for the expression... 6. 106(1):157-62. Jaenisch Lentivirus Mouse Doxycycline-inducible lentiviral expression of mouse Oct4...Aug 25. 126(4):663-76. Yamanaka piggyBac Mouse Doxycycline-inducible piggyBac transposon reprogramming system... 2011 Mar;21(3):518-29. Cheng piggyBac Mouse Doxycycline-inducible piggyBac transposon reprogramming system...

... The Design-Build-Test-Learn cycle adapted for video making.   Cycle One: The Ironing Board Control...it was time to start our Design-Build-Test-Learn cycles. If you’re not familiar with this, it’s a method... WFH desk, and as a video control center.   Cycle 2: Zooming Forward Design: We produced a video celebrating...shot composition.  So back to the drawing board.  Cycle 3: A Return to HQ Figure 3: Even Blugene...in a different room.   Design: The previous cycle made me realize we needed to focus on ways to shoot... be in there with them. I knew from my previous cycles that I wanted to be able to talk to people and ...camera control, and extra hand sanitizer.    Cycle 4: Venturing into the Lab Design: The lab is the...

...molecule often work better for rapid-cycle PCR, such as the LightCycler, as it reduces the primer/probe actions...This separate thermocycle allows you to measure the intensity of signal over an entire cycle. Since signals...especially if you’re using a rapid-cycle machine such as a LightCycler, you’ll likely need to re-optimize...particular has been reported to work well in the Lightcycler while being relatively easy to multiplex (Thellwell...unless running a multiplex reaction or on a rapid cycle machine. Melt Curves An advantage of Sybr chemistry...paper for more information on optimizing for LightCycler machines.) Multiplexing It is possible to multiplex... IA; Choi JW;  Morlese J; Taylor G; Shaun S. LightCycler qPCR optimisation for low copy number target ...

...reaction cycle. However, it is common for dNTPs and other reagents to run low during the final cycles which...baths but is now easily done in a programmed thermocycler. For more details on this procedure check out...implies, analyzes the end product of PCR temperature cycling. The final PCR product is often visualized on a... use of a fluorometer feature built into the thermocycler to measure that fluorescent output. With this...concentration of the DNA during the PCR reaction cycles is continuously detected via a fluorescent signal...proportionally to the amount of product produced each cycle.  To determine the concentration of the starting...amplified DNA of a known starting concentration. The cycle in which the unknown DNA is detected compared to...

...Overexpression Tracers Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible tools...tools/controls and tetracycline transactivators that can be used with tetracycline (tet)-inducible expression...Viral Vector Packaging Service: Molecular Tools Tetracycline Transactivators Affinity Purification Neurophysiology...Gradinaru 117383 TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible Cre-dependent, Tet-inducible expression...

...integrase.  The IDLV life cycle When IDLVs are delivered to cells, the viral life cycle initially proceeds as...retroviridae, require integration for their life cycle to continue. They rely on a viral protein called...production (read our blog post on the retroviral life cycle for a more detailed description). Since the integrated...contain any of the viral packaging genes, the natural cycle of the lentiviral vector ends here and no viral ...Figure 1).  Figure 1: Comparison of the life cycle of a lentiviral vector with an integrase-deficient...expression diminishes as the episomes are lost through cycles of cell division, though the rate of loss greatly...Addgene blog Viral Vectors 101: The Retroviral Life Cycle Quick Guide to All Things Lentivirus More resources...

...its native context, the tetracycline repressor (TetR) can bind to the tetracycline operator sequences (TetO...preventing transcription. In the presence of tetracycline (Tet), TetR preferentially binds Tet over the...repressible system, Gossen and Bujard created the tetracycline-controlled transactivator (tTA). They fused ...TRE)), allowing transcription to proceed. When tetracycline or one of its derivatives is added, it binds...information on Addgene Tet plasmids, see our Tetracycline resource page. Cumate Switch Massie et. al developed...control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci U S...Signals FLEx Vectors Resources at Addgene.org Tetracycline Collection ...

...HDR events, ensure cells are actively cycling through the cell cycle stages. If cells are overly confluent...across cell types. Variance can be caused by cell cycle rates; rapidly diving cells will have higher HR ...operates predominantly in S and G2 phase of the cell cycle, when a sister chromatid is likely to exist as a...take this one step further, you can inhibit cell cycle regulatory proteins responsible for the transition...out of S phase, so cells stay in an HDR-promoted cycle longer. This approach may have some negative outcomes... if cells are arrested too long. Prolonged cell cycle arrest can lead to apoptosis in as little as 10 ...

...plastic and packaging material or by incorporating recycled paper and plastic. Aside from the more obvious...obvious swaps like using smaller bottles or recycled cardboard packaging, there are some less obvious improvements... Waste tubes are made of 100% post-consumer recycled plastic Reduced plastic in buffer bottles ... from packaging Made from 100% post-consumer recycled material Reduced cardboard packaging Plant-based... cardboard packaging by more than half 70% recycled materials Sustainable forestry certification Starch-based...Starch-based compostable bags for kit components Recyclable Yes, all kit components and packaging Yes...

...reverse tetracycline-controlled transactivator) is normally inactive and cannot bind the tetracycline response... inducible promoters. The positive inducible tetracycline ON (Tet-On) system, a versatile tool developed...response elements (TRE) in a promoter. Tetracycline and its derivatives serve as inducing agents to allow...Addgene.org: Inducible Promoters for Bacteria Tetracycline (Tet) Inducible Systems at Addgene Cre-lox Guide...