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  1. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...bandstands: cognitive load.  Cognitive load The Wikipedia definition of cognitive load states: “cognitive load...resources. There are three types of cognitive load: intrinsic cognitive load is the effort associated with...with a specific topic; extraneous cognitive load refers to the way information or tasks are presented to... to a learner; and germane cognitive load refers to the work put into creating a permanent store of knowledge... (a schema).”  In scicomm, I like to think of cognitive load as the amount of mental thought and effort...s quite easy (and common!) to be blind to the cognitive load you’re placing on your audience when you'...enthusiastic of learners.  What contributes to cognitive load?  Everything! Here are some common areas ...
  2. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...naturally occurring bacterial endonucleases that recognize a large range of DNA sequences. Given the variety...variety of these enzymes and the unique sites they recognize, restriction digests have become the most widely...
  3. Antibody Guide

    Type
    Collection
    ...capable of recognizing and binding to epitopes on antigens, they are also capable of recognizing and binding...source secondary recognizes], e.g. , “goat anti-rabbit.” Choose a secondary that recognizes your primary ...proteins, naturally produced by the immune system to recognize and bind foreign bodies. Antibodies can be used...proteins of interest. The protein that an antibody recognizes is called an antigen, while the specific binding... probability that an antibody will be able to recognize and bind to any given antigen. A mature B cell...specificity in antibody-based methods. Antibodies can recognize and bind other antibodies using epitopes present...important to choose a monoclonal antibody that will recognize an available epitope on your protein of interest...
  4. TALEN Guide

    Type
    Collection
    ... of a zinc finger (ZF) nuclease. Zinc fingers recognize triplets and the FokI nuclease operates as a dimer... the ZF nuclease, the components of the array recognize individual nucleotides. For over ten years, zinc...identified a large number of zinc fingers that recognize various nucleotide triplets. With some trial and... and error, ZF arrays are able to recognize their targets with fairly high specificity. Fifteen years ...Iowa State University published the nucleotide recognition code of the TAL effectors, which were isolated...which single nucleotide the TAL effector will recognize: ( Figure 1B ) HD targets cytosine, NI targets...
  5. Genetic Code Expansion

    Type
    Collection
    ...codon (typically the amber codon) that is recognized by the cognate charged tRNA. Once these plasmids have... an aminoacyl-tRNA synthetase (aaRS) loads its cognate tRNA with a specific amino acid. Then, the tRNA...figure below: A plasmid expressing the tRNA and its cognate aminoacyl-tRNA-synthetase (aaRS) that has been ... codons, have also been utilized. A tRNA that recognizes this codon. An aminoacyl-tRNA synthetase to load...plasmids also contain one or more copies of the cognate tRNA gene. ID Plasmid Synthetase Origin ncAA Expression...
  6. CRISPR Guide

    Type
    Collection
    ...regions, as it recognizes TTTV (V = A, C, or G) PAM sites. Engineered versions of Cas12a recognize different... mutations in multiple domains to expand PAM recognition, increase specificity, and decrease off-target...Faecalibaculum rodentium ) has increased fidelity, and recognizes NNTA, a PAM sequence often found in plants, expanding...mechanisms, including blocking crRNA loading or PAM recognition; inhibiting Cas9’s endonuclease domain; or inducing...EnCas12a, an engineered version of AsCas12a that recognizes TYCV and TATV PAM sites. The DETECTR platform...using Cas13 Cas13 is a type VI CRISPR enzyme that recognizes ssRNA. Type VI enzymes, which include Cas13a ...crRNA precursors to mature crRNAs. Upon ssRNA recognition by the crRNA, the target RNA is degraded. In ...
  7. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...iChIP, specific genomic regions tagged with the recognition sequences of an exogenous DNA-binding protein...pCMV-7.1 Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat 63590 3xFN-Tel-TAL/pMXs-puro...pMXs-puro Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector...pMXs-neo Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector...
  8. Immunology Research Plasmids and Resources

    Type
    Collection
    ... gfg, gfg-1 OGFR opioid growth factor receptor - OGN osteoglycin DKFZp586P2421, OG, OIF, SLRR3A OPRD1 ...triggers several processes to activate cells to recognize and get rid of infectious pathogens. The innate...immunity. Once an adaptive immune cell is primed to recognize a specific antigen, it finds and attacks pathogens...expand and give rise to memory cells that can recognize a recurrent infection and rapidly initiate a secondary...The cornerstone of the immune response is the recognition by T cells of foreign antigens on the surface...presenting cells. B cells in secondary lymphoid organs recognize specific antigens through the B cell receptor ...Engagement of the TCR by an MHC molecule presenting its cognate antigen along with co-stimulation activates the...
  9. Deisseroth INTRSECT Collection

    Type
    Collection
    ...placing two orthogonal recombinase (Cre and Flp) recognition sequences within synthetic introns. INTRSECT ...) of a molecular tool and adding recombinase recognition sequences (e.g. Lox sites, FRT sites) inside ...introns (A,D). The addition of these recombinase recognition sequences enables directional control of the ...
  10. Ligation Independent Cloning

    Type
    Protocol
    ...which cut at a specified distance from their recognition sequence. This can create multiple distinct overhangs...
  11. mTOR Pathway

    Type
    Collection
    ...key metabolic regulator, correlates with poor prognosis in many cancers.... either mTORC1 or mTORC2 correlates with poor prognosis in many cancers, including breast, colon, ovarian...
  12. Cre-lox system

    Type
    Collection
    ... gene expression. The protein Cre recombinase recognizes 34 bp loxP sites, and the orientation and location...and SCre are alternative Cre recombinases that recognize specific mutant loxP sites, VloxP and SloxP sites...the control of gene expression. Flp recombinase recognizes FRT sites. Flp binding to these sites can result...
  13. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ... rather than DNA. In bacteria, once they have recognized and cleaved the target RNA sequence, they adopt...
  14. Institute for Protein Innovation

    Type
    Collection
    ...developed recombinant versions of antibodies that recognize common epitope tags to help purify, detect, or...
  15. Antibody Production

    Type
    Collection
    ...in-house or through outside labs, to confirm recognition of the expected target or to confirm expected...
Showing: 1 - 20 of 258 results