We narrowed to 717 results for: CaS;

...per 10 cm dish). Using transfer plasmid pHAGE TRE dCas9-KRAB (total μg of plasmid DNA 27.8 μg), this would...

...shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Luk Parijs ...shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Stephan Kissler... plasmid with a chimeric 5’LTR. Any expression cassette (promoter and gene of interest) can be cloned ...interest. Can also express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in the 3´-LTR). Find...

... or can come preloaded into tip boxes. In either case, once assembled into their boxes, pipette tips can...

...modifications, researchers have fused catalytically dead Cas9 (dCas9) to epigenetic modifiers. Common modifiers include...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you ...

...Promoter PI Publication 64325 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO-1 Fujii Efficient isolation of...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you ...

...research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and forward...forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for some...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you ...

...chamber. Use the metal spatula to gently break the gel cast open. Use a razor blade to cut the top of the wells...

...-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP U6 ALS...Patrick Caswell 179900 pJET-59-DExCon-mTagBFP2-R25 RAB25 mTagBFP2 TRE3GS Parkinson's Patrick Caswell 180016...sgHTT1-7sk-sgCas9 HTT U6, 7sk Huntington's Nicole Deglon 190901 pAAVss-U6-sgHTT51-7sk-Cas9 HTT U6, 7sk...MS2 CMV Parkinson's Niels Gehring 66818 pCCL-PGK-SPdCas9-BFP-DNMT1 DNMT1 PGK Hereditary sensory neuropathy...pEGFP-C9orf72 C9orf72 GFP CMV ALS Shawn Ferguson 83439 pSpCas9(BB)-2A-Puro-C9orf72 C9orf72 U6 ALS Shawn Ferguson...Episodic ataxia Xiaolong Wang 159912 pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3 HTT CMV Huntington's Larry Zweifel 160435...Wernig 176852 UBQLN2_450-624W UBQLN2 T7 ALS Carlos Castaneda 176915 pcDNA3.1(+)_Paragranulin+linker 1 GRN Flag...

...expression. Monoclonal antibodies are necessary in cases where extreme specificity is important. Their epitope...when epitope specificity is not a concern or in cases where you may want to identify or collect the protein...azide, as it acts as an inhibitor to HRP. In those cases, thimerosal can be used as an antimicrobial agent... using the above process and try again. In some cases, it may be preferable to buy small aliquots of many...

... Prepare one to two more conical tubes on ice in case you need to spread out the harvested cells further...

...webpages. These plasmids are described in Portales-Casamar et al., 2010 . List of Pleiades MiniPromoters MiniPromoter...promoter pEMS1488 intron-lacZ/NLS References Portales-Casamar, E., Swanson, D. J., Liu, L., de Leeuw, C. N., ...Bonaguro, R. J., Brauer, E., Candido, T. R., Castellarin, M., Chen, J., Chen, Y., Cheng, J. C., Chopra...

...protein expression in the stationary phase. In these cases, you may want to control protein levels or to turn...Plasmid Promoter Inducer Expression Species PI 44249 pdCas9-bacteria pTetO Anhydrotetracycline (aTc) Escherichia...synthesize a target metabolite, genetic tools like CRISPR-Cas9 are not only valuable but often essential. Find ...

...Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes KLF4 N-terminal variance modulates induced reprogramming...Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes. piggyBac transposition reprograms fibroblasts...CRISPR Mouse Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice...

...photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV8 pAAV-Ef1a-DIO-PPO-Venus...photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV9 pAAV-Ef1a-DIO-PPO-Venus...photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 140135-AAV1 pAAV-EF1a-iCreV can...

...titered by droplet digital PCR (ddPCR) . In some cases, we may expect lower titers for a particular AAV...titer due to toxicity or other reasons For these cases, Addgene will promptly notify you by email within...

...19 research, including: CRISPR tools, like Cas12a and Cas13a plasmids, to reliably detect nucleic acids...

...the stuffer. The AgeI site is destroyed in most cases (depending on the target sequence), while the EcoRI...

...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you ...

...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you ...

...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you ...