Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 716 results for: cas

Showing: 221 - 240 of 716 results

  1. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...contains Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...transcriptase (RT) is fused to the C terminus of Cas9 H840A nickase. The fusion enzyme is capable of installing...template. During prime editing, the pegRNA directs the Cas9 nickase to the target sequence where it nicks the...nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can be used in PE2...PE6 - small prime editors with optimized RT and/or Cas9 domains PE7 - addition of RNA-binding exonuclease...PuroTK-pegRNA_GG Mammalian, piggyBac hU6 pegRNA BsmBI Yes (Cas9 H840A + MMLV RT) PuroTK Jacob Giehm Mikkelsen 174038...

  2. Fluorescence Titering Assay

    Type
    Protocol
    ...transduced with a range of dilutions of pHAGE-TO-dCas9-3XmCherry . 72 h post transduction, cells were assayed...

  3. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...require the use of lentivirus to deliver the gRNA/Cas9 to target cells. Therefore, you must be equipped...Doench and Root 2nd 2 40,710 Inzolia Human CRISPR/Cas12a Multiplex Knockout 209551 209552 Knockout Human... and Root 3rd 3–6 67,366 (Set A) 67,194 (Set B) Cas13a/C2c2 Protospacer flanking site (PFS) Library 79153...86550 Inhibition Human Weissman 3rd 10 Varies CRISPR/Cas9-assisted Removal of Mitochondrial DNA (CARM) Library... Sabatini and Lander 3rd 50 6,661 Garnett Lab MinLibCas9 Library 164896 Knockout Human Garnett 3rd 2 37,722...Human Stiewe N/A Varies 1,910–3,034 per exon Human Cas13 lncRNA Library 227014 RNA Knockdown Human Sanjana...

  4. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...inactive dCas9 is used to purify genomic DNA bound by the gRNA. An epitope tag(s) can be fused to dCas9 or ... for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9 can be achieved by fusing a biotin...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system. The ...purification, design your gRNA sequence to direct dCas9 to a specific locus, avoiding known transcription...

  5. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ... protein dCas9 is expressed along a CRISPR array that contains 20 bp "addresses", where dCas9 is going...going to bind on the chromosome. When dCas9 binds to a gene, it blocks the progression of the RNA polymerase...for quick integration of the aTc-inducible pTet-dCas9 in the attachment site of the phage 186. Detailed... Bikard, D., & Van Teeffelen, S. (2018). Tuning dCas9’s ability to block transcription enables robust,...Schematic of the strain expressing two reporters and dCas9 under a P-tet promoter integrated in the chromosome... of the two reporters, mCherry and sfgfp in this case, can be controlled using a plasmid‐borne CRISPR ...

  6. AAV Molecular Tools

    Type
    Collection
    ...Engineering Tools These AAV encode Cas9 nuclease used in the CRISPR-Cas9 system for targeted genome editing...AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA CMV-driven, constitutive Expression of human codon-optimized Cas9 from Staphylococcus...cell ablation. 2 Jessell , Azim 45580 pAAV-flex-taCasp3-TEVp EF1a-driven, Cre-dependent Cre-dependent, ..., bicistronic expression of designer pro-taCasp3 and TEVp for studying cell ablation. 1, 5 Shah , Wells...Staphylococcus aureus (SaCas9). 6 Zhang Labeling Tools These AAV encode tools that can be used for cell...

  7. Validated gRNA Sequences

    Type
    Collection
    ...single species or PAM binding variant of Cas9 (e.g. Wild-type SpCas9 must be used with targets that are upstream...activity. Know your target. Which species or variant of Cas9 ( S. pyogenes, S. aureus etc.) was this gRNA sequence...and Resources page have been used to indicate the Cas9 application the gRNA was designed to accomplish....Target Species Target Sequence Plasmid ID Application Cas9 Species PubMed ID Depositor OCT4 H. sapiens CTCCCATGCATTCAAACTG...10 C. elegans CGCTACCATAGGCACCACG 71516 cut VRER Cas9 mutant 26680661 Fire dpy-10 C.elegans GCTACCATAGGCACCACGAG...-10 C. elegans TCCGCTACCATAGGCACCA 71479 cut VQR Cas9 variant 26680661 Fire dpy‐10 C. elegans GCTACCATAGGCACCACGAG...Christiaen EGFP A. victoria multiple, see article 60071 dCas9-FokI S. pyogenes 24770325 Joung EGFP A. victoria...

  8. Neurodegeneration Research Collection

    Type
    Collection
    ...familial ALS cases having at least one other affected family member. These familial cases, usually inherited...range of cellular processes. The vast majority of cases (90-95%) of ALS are sporadic, having no prior family...cause) nature of the disease, a small portion of cases can be attributed to known genetic factors such ...early-onset form accounts for less than 10 percent of all cases of Alzheimer's disease. Of these early-onset forms...Target neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent rodent...species. Boender et al. Sci Adv. 2023 Jun 2. Use Cas9 in astrocytes. Endo et al Science. 2022 Nov 4. Base...

  9. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... an exogenous donor cassette, the donor cassette replaces the target site cassette in a double-crossover... sites (often called a "lox-STOP-lox" or "LSL" cassette) placed upstream of a gene of interest will prevent...-layered genetic control. Recombinase-mediated cassette exchange (RMCE): When matching but mutually incompatible...vivo using a mouse line expressing Cre-dependent Cas9. Kits Kit Name Type PI Description dRMCE Plasmid...Rolf Zeller Use this dual recombinase-mediated cassette exchange (dRMCE) plasmid kit to re-engineer conditional...

  10. Lentivirus Production

    Type
    Protocol
    ...per 10 cm dish). Using transfer plasmid pHAGE TRE dCas9-KRAB (total μg of plasmid DNA 27.8 μg), this would...

  11. p53 Pathway

    Type
    Collection
    ...domain death agonist CASP3 Caspase 3, apoptosis-related cysteine peptidase CASP8 Caspase 8, apoptosis-related...apoptosis-related cysteine peptidase CASP9 Caspase 9, apoptosis-related cysteine peptidase Cyclin B CCNB1 CCNB2 CCNB3...

  12. Pipetting Protocol

    Type
    Protocol
    ... or can come preloaded into tip boxes. In either case, once assembled into their boxes, pipette tips can...

  13. Immunology Research Plasmids and Resources

    Type
    Collection
    ...mE10 CARD11 caspase recruitment domain family, member 11 BIMP3, CARMA1, MGC133069 CBL Cas-Br-M (murine...retroviral transforming sequence C-CBL, CBL2, RNF55 CBLB Cas-Br-M (murine) ecotropic retroviral transforming sequence...DKFZp779F1443, FLJ36865, FLJ41152, Nbla00127, RNF56 CBLC Cas-Br-M (murine) ecotropic retroviral transforming sequence...BRAF1, FLJ95109, MGC126806, MGC138284, RAFB1 CASP3 caspase 3, apoptosis-related cysteine peptidase CPP32...ASBT, ISBT, NTCP2 TAP1 transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) ABC17, ABCB2, APT1, D6S114E...TAP1*0102N, TAP1N TAP2 transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) ABC18, ABCB3, APT2, D6S217E...IMD1, MGC126261, MGC126262, PSCTK1, XLA CARD11 caspase recruitment domain family, member 11 BIMP3, CARMA1...

  14. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... FP Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions...create stable cell lines in which the expression cassette of interest is incorporated into the host...Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression pnCasPA-BEC - A cytidine deaminase-mediated base-editing... Separate expression pRGEB31 - Deliver sgRNA and Cas9 into plants by Agrobacterium-mediated transformation...Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated...

  16. Rett Syndrome

    Type
    Collection
    ...typically arise spontaneously, although in rare cases Rett syndrome can be inherited. In females, random...R306C) account for almost 46% of all Rett syndrome cases. Research Milestones 1992 - MECP2 is discovered ...Knock-in of NLuc-tdTomato at endogenous MECP2 locus Castaneus MECP2-NLuc-tdTomato mouse reporter cell line (...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of additional...hand use in girls: Rett’s syndrome: report of 35 cases. Ann Neurol . 14, 471–479. (Link opens in a new ...

  17. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...markers. The FP together with the protothropic cassette can be amplified with the following primers: FW...Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas Teusink Lab Materials... Sheff, M. A., Thorn, K. S. (2004). Optimized cassettes for fluorescent protein tagging in Saccharomyces...., Nielsen, L. K. (2013). Dual gene expression cassette vectors with antibiotic selection markers for ...

  18. Lentivirus Plasmids

    Type
    Collection
    ...shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs 11619...shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler 1730... plasmid with a chimeric 5’LTR, Any expression cassette (promoter and gene of interest) can be cloned ...interest, also can express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in the 3´-LTR). See...

  19. CRISPR Library Amplification

    Type
    Protocol
    ... Prepare one to two more conical tubes on ice in case you need to spread out the harvested cells further...

  20. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP U6 ALS...Patrick Caswell 179900 pJET-59-DExCon-mTagBFP2-R25 RAB25 mTagBFP2 TRE3GS Parkinson's Patrick Caswell 180016...sgHTT1-7sk-sgCas9 HTT U6, 7sk Huntington's Nicole Deglon 190901 pAAVss-U6-sgHTT51-7sk-Cas9 HTT U6, 7sk...MS2 CMV Parkinson's Niels Gehring 66818 pCCL-PGK-SPdCas9-BFP-DNMT1 DNMT1 PGK Hereditary sensory neuropathy...Episodic ataxia Xiaolong Wang 159912 pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3 HTT CMV Huntington's Larry Zweifel 160435...Wernig 176852 UBQLN2_450-624W UBQLN2 T7 ALS Carlos Castañeda 176915 pcDNA3.1(+)_Paragranulin+linker 1 GRN...Parkinson's Frank Soldner 180437 Px330-EGFP-LRRK2-CRISPR/Cas9 LRRK2 U6 Parkinson's Frank Soldner 180439 STR-KDEL_PGRN-SBP-mCherry...
Showing: 221 - 240 of 716 results